Storage and Quality Assessment of Platelets

Abstract

Platelet concentrates stored for 5 days under currently optimal conditions at 22 C demonstrate approximately a 20-30% loss of viability as determined by CCI measurements in thrombocytopenic patients and radiolabeling studies with healthy volunteers. The functionality of platelets is substantially lost during storage. This loss appears, for most part, to be reversible upon infusion. In view of this, stored platelets should be acceptable for prophylactic treatment of thrombocytopenic patients in whom immediate hemostatic function is not critical. However, whether stored platelets are functionally adequate for immediate arrest of perioperative bleeding in surgical patients remain uncertain. Currently, a fully adequate and appropriate test that evaluates the hemostatic function of a stored platelet product is not available. In view of this, a desired goal in research and development of new platelet products may then be to attain a platelet product that is similar to fresh platelets in various haemostatic functions. Whether this is an obtainable or relevant goal remains to be seen.

Platelets: Testing, Dosing and the Storage Lesion—Recent Advances

“…It has been shown that if the pH drops below 6.0-6.2, survival in vivo is severely diminished. 26 Platelets also tend to become activated during storage. Over time, an increasing fraction of platelets in a concentrate will change from a discoid (resting) shape to spherical.…”

“…It has long been recognized that platelet viability and function decline over time, the so-called "platelet storage lesion." 26,27 The most compelling data pointing to damage incurred during platelet storage come from radiolabeling studies in autologous volunteer platelet donors. Most investigations have shown that platelets transfused following storage have a significantly decreased recovery and survival relative to fresh platelets.…”

“…Most investigations have shown that platelets transfused following storage have a significantly decreased recovery and survival relative to fresh platelets. 26,28,29 For example, day 5 and day 7 platelets have been compared directly in radiolabeling studies. Day 5 platelets were found to have a mean recovery of 63% and a mean survival of 160.8 hours, compared with day 7 platelets, which had a recovery and survival of 53.9% and 133.6 hours, respectively.…”

Comparison of harvesting methods and clinical application of apheresis platelet concentrates with additive solution

“…Процедура заготовки и хранения тромбоцитов приводит к выраженным изменениям их морфологи-ческих и функциональных характеристик, что в свою очередь ведет к снижению терапевтической эффектив-ности трансфузионной терапии [28,29]. Это явление известно как «повреждение тромбоцитов при хране-нии» (platelets storage lesion, PSL).…”

Quality Assessment of Platelet Concentrate Preparations at the Nairobi Regional Blood Transfusion Centre in Kenya

“…Platelet concentrates are randomly prepared from platelet rich plasma derived from WB of donors Nor Raihan M (3) . Withcontinuous agitation, platelets concentrates can be stored for 5 days at 22 ± 2°C(Njoroge (4) HolmeS (5) , G. Moroff (6) , Welch M (7) ). The AABB (1) is tasked with the responsibility of setting standards for transfusion medicine on blood collection, spinning, platelet separation, storage and agitation.…”