Stopped flow kinetic determination of nalidixic acid and norfloxacin based on lanthanide-sensitized fluorescence

Panadero, S.; Gómez‐Hens, A.; Pérez‐Bendito, Dolores

doi:10.1016/0003-2670(94)00487-7

Cited by 43 publications

(20 citation statements)

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“…It was reported that terbium and europium ion can form complexes with similar antibacterial agents such as norfloxacine, nalidixic acid, ciprofloxacine, gatifloxacine, and moxifloxacin. [26][27][28][29] In our previous study we have successfully determined adenosine disodium triphosphate (ATP) using Eu…”

Section: -25mentioning

confidence: 99%

Europium-Enoxacin Complex as Fluorescence Probe for the Determination of Folic Acid in Pharmaceutical and Biological Samples

Alam¹,

Kamruzzaman²,

Lee³

et al. 2012

Bulletin of the Korean Chemical Society

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A simple, rapid and sensitive spectrofluorometric method was developed for the determination of folic acid (FA), based on its quenching effect on the fluorescence intensity of enoxacin (ENX)-europium (Eu 3+) complex as a fluorescent probe. Fluorometric interaction between ENX-Eu 3+ complex and FA was studied using UVvisible and fluorescence spectroscopy. The quenched fluorescence intensity at an emission wavelength of 614 nm was proportional to the concentration of FA. Optimum conditions for the determination of FA were investigated. Under optimal conditions, the reduced fluorescence intensity at 614 nm was responded linearly with the concentration of FA. The linearity was maintained in the range of 1.25 × 10 −9 to 1.50 × 10 −7 M (R = 0.9986) with the limit of detection (3S b /m) (where S b is the standard deviation of blank and m is the slop of linear calibration curve) of 6.94 × 10 −10 M. The relative standard deviation (RSD) for 9 repeated measurements of 1.0 × 10 −9 M FA was 1.42%. This method was simple, cost effective, and relatively free of interference from coexisting substances. Successful determinations of FA in pharmaceutical formulation and biological samples with the developed method were demonstrated.

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Section: -25mentioning

confidence: 99%

Europium-Enoxacin Complex as Fluorescence Probe for the Determination of Folic Acid in Pharmaceutical and Biological Samples

Alam¹,

Kamruzzaman²,

Lee³

et al. 2012

Bulletin of the Korean Chemical Society

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“…No intuito de obter um sinal fluorescente, a formação de complexos entre o fármaco e outras espécies tem sido bastante explorada por muitos grupos de pesquisa 15,74,78,[94][95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110]117,132 (Tabela 3). Muitos artigos têm demonstrado que a inclusão do fármaco dentro da cavidade de ciclodextrinas pode induzir a emissão de várias moléculas.…”

Section: Complexos Fluorescentes De Fármacosunclassified

“…Complexos ternários formados na presença de íon térbio (III), o fármaco, e moléculas de óxido de trioctilfosfina 100 ou moléculas de tris-hidroximetilaminoetano (TRIS), 101 ou EDTA em meio alcalino 103 também oferecem uma forma de quantificar alguns fármacos em formulações comerciais. A capacidade que possuem alguns fármacos derivados de quinolonas e quinolinas para formar com metais de transição complexos fluorescentes tem sido explorada na determinação desses reagentes fluorogênicos (fármacos).…”

Section: Complexos Fluorescentes De Fármacosunclassified

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

et al. 2008

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Recebido em 20/10/06; aceito em 30/11/07; publicado na web em 4/9/08 APPLICATION AND ADVANCES IN THE LUMINESCENCE SPECTROSCOPY IN PHARMACEUTICAL ANALYSES. This paper provides a review on the latest advances and applications of the luminescence spectroscopy for the development of pharmaceuticals analyses methods, basically based on the photo-and chemiluminescence. The different forms of the drugs determination on pharmaceuticals through the fluorescence and chemiluminescence are discussed. The analyses include the drugs native fluorescence (liquid and solid-phases); the fluorescence from the oxidizing or reducing forms of the drug; the fluorescence from the chemical derivatization and their photochemistry and hydrolysis reactions. The quenching of luminescence and chemiluminescence generation for the pharmaceutical quantification are also shown. Finally, the trends and future perspectives of the luminescence spectroscopy in the field of the pharmaceutical research are discussed.Keywords: luminescence spectroscopy; drugs; cosmetics analysis; introdução Devido ao progresso contínuo em técnicas e instrumentos, a indústria farmacêutica busca o emprego de metodologias que ofereçam maior sensibilidade, seletividade, robustez, precisão, exatidão e rapidez. Neste sentido, um grande número de equipamentos de cromatografia líquida de alta eficiência (CLAE) com diversos tipos de detecção domina este ramo industrial. Contudo, o advento da sofisticação de instrumentação automatizada de espectrômetros de luminescência poderá resultar em um substancial aumento de aplicações desta técnica instrumental na indústria farmacêutica, principalmente, no que se refere à análise diretamente na matriz do medicamento.A espectroscopia de luminescência é uma ferramenta analítica extremamente sensível e tem sido amplamente aplicada na resolução de problemas que requerem baixos limites de detecção, podendo ser facilmente encontrados na literatura muitos trabalhos mostrando análises ao nível de traços.1-6 A sensibilidade inerente a esta técnica é consideravelmente maior em comparação a outras metodologias, entre elas a espectrofotometria UV/visível, apresentando limites de detecção de até três ordens de grandeza menores, os quais normalmente se encontram na faixa de ng mL -1 , sendo conseqüência do baixo sinal de fundo que as medidas fluorescentes apresentam. Diferentemente do que ocorre no fenômeno da luminescência, nas medidas de absorbância o sinal está associado à atenuação da intensidade de um feixe de radiação por uma espécie absorvente. Nos casos onde a quantidade da espécie é pequena, esta atenuação é menor, tornando assim a detecção da espécie difícil. Outra característica importante da luminescência diz respeito à seletividade, porque espécies com rendimentos quânticos de fluorescência apreciáveis são menos comuns do que substâncias cuja fluorescência não pode ser detectada. Desta forma, moléculas fluorescentes apresentam comprimento de onda característico de excitação e/ou emissão. Adicionalmente, o comprimento de onda de excitação d...

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“…This terbium-sensitized method has been used in the determination of FQs. [7][8][9][10][11] In recent years, the light scattering technique has been increasingly applied to analytical chemistry, the most common method used is resonance light scattering (RLS). In studying RLS, the strong scattering of light at double the wavelength of the incident light (λem = 2λex) is found, which is named as "second-order scattering (SOS)".…”

Section: Introductionmentioning

confidence: 99%

Determination of Ulifloxacin by Terbium-sensitized Fluorescence with Second-order Scattering and Its Applications

et al. 2007

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IntroductionAs broad-spectrum antibiotics, fluoroquinolones (FQs) have gained increasing use and have become popular in clinical application thanks to their high bacteriologic and clinical cure rates and good pharmacokinetic profile. 1 Prulifloxacin (PUFX, Scheme 1), 6-fluoro-1-methyl-7-(4-[5-methyl-2-oxo-1,3-dioxolen-4-yl]-methyl-1-piprazinyl)-4-oxo-4H-(1,3)thiaceto(3,2-α)quinolone-3-carboxylic acid, is a new oral fluoroquinolone antibiotics. It is active against both gram-positive and gram-negative bacteria and several anaerobic and atypical bacteria associated with chronic bronchitis and urinary infections. 2After PUFX is administrated, it is immediately and quantitatively transformed to the active metabolite ulifloxacin (UFX, Scheme 1) which actually has antibacterial activity. Prulifloxacin is not detectable in the systemic circulation. 3 That is to say, PUFX is the prodrug of UFX. The oxodioxolenylmethyl group in the 7-piperazine ring helps PUFX to have an advantage over UFX on oral absorption.3 Thus, the main form existing in biological fluids, such as serum and urine, is UFX. It is thus necessary to develop rapid, selective and sensitive analytical methods for the determination and pharmacokinetic study of UFX.Analytical literature about the determination of UFX is seldom seen. There are a few papers that describe HPLC methods. 4-6The HPLC methods allow the separation and generally require expensive equipment and complicated procedures. The main advantages of fluorometry are simplicity and rapidity. Luminescence sensitization of lanthanide ions (especially Tb(III) and Eu(III)) allows their application as the luminescence probes. Lanthanide-sensitized luminescence provides a selective and sensitive approach that shows a large stokes shift, narrow emission bands and a long luminescence lifetime. The carboxylate and the keto oxygen atoms of FQs provide the chelated sites for Tb(III) or Eu(III) ion to form Ln(III)-FQ complex. When Tb(III)-FQ complex is excited, it gives rise to the characteristic luminescence of Tb(III) ion with maximum emission at 545 nm via intramolecular energy transfer. This terbium-sensitized method has been used in the determination of FQs. 7-11In recent years, the light scattering technique has been increasingly applied to analytical chemistry, the most common method used is resonance light scattering (RLS). In studying RLS, the strong scattering of light at double the wavelength of the incident light (λem = 2λex) is found, which is named as "second-order scattering (SOS)". 12 As it may interfere with fluorometric measurements, SOS is always minimized and if possible eliminated as a harmful phenomenon in the fluorometric determinations. 13 Since Liu et al. first studied SOS as an analytical technique and applied it in the determination of metal ions in 1995, 14,15 however, it has been used as a new analytical technique with sensitive application prospects. 16,17 We have reported some flow-injection lanthanide-sensitized chemiluminescence (CL) [18][19][20] and electrochemiluminescence ...

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Stopped flow kinetic determination of nalidixic acid and norfloxacin based on lanthanide-sensitized fluorescence

Cited by 43 publications

References 12 publications

Europium-Enoxacin Complex as Fluorescence Probe for the Determination of Folic Acid in Pharmaceutical and Biological Samples

Europium-Enoxacin Complex as Fluorescence Probe for the Determination of Folic Acid in Pharmaceutical and Biological Samples

Aplicação e avanços da espectroscopia de luminescência em análises farmacêuticas

Determination of Ulifloxacin by Terbium-sensitized Fluorescence with Second-order Scattering and Its Applications

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