Stool-based DNA testing, a new noninvasive method for colorectal cancer screening, the first report from Iran

Abbaszadegan, Mohammad Reza; Tavasoli, Alireza; Velayati, Arash; Sima, Hamid Reza; Vosooghinia, Hassan; Farzadnia, Mehdi; Asadzedeh, Hamid; Gholamin, Mehran; Dadkhah, Ezzat; Aarabi, Azadeh

doi:10.3748/wjg.v13.i10.1528

Cited by 39 publications

(32 citation statements)

References 23 publications

(26 reference statements)

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“…Different methods such as light 1989;Zhang et al 2012). Some of these approaches were ineffective due to difficulty in releasing DNA from cysts, and also due to the presence of many compounds in stool samples such as proteases, DNase, polysaccharides and bile salts, which degrade DNA and inhibit enzymatic reactions (Abbaszadegan et al 2007). The tpi gene locus for development of specific PCR assay was chosen in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Identification of G. duodenalis genotypes in clinical specimens as well as differentiation of Giardia at the assemblage and genotype levels have relied on the analysis of the SSU-RNA, b-giardin (bg), glutamate dehydrogenase (gdh), elongation factor 1-alpha (ef-1), triose phosphate isomerase (tpi) genes. Molecular approaches to further discrimination of G. duodenalis isolates are reliant on the efficient extraction of DNA from Giardia cysts (Abbaszadegan et al 2007;Machiels et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Comparison and evaluation of four methods for extracting DNA from Giardia duodenalis cysts for PCR targeting the tpi gene

et al. 2016

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Giardia duodenalis is an intestinal flagellated protozoan and the common cause of gastrointestinal diseases in human. This parasite can be seen in two different forms in its life cycle including as cyst and trophozoite. Due to presence of resistant cyst wall, DNA extraction inhibitors along with artifact in stool specimens, this study was performed aiming to evaluate four methods for DNA extraction from G. duodenalis cysts. Seventy G. duodenalis positive stool specimens that were confirmed by light microscope were included in this study. All stool samples were concentrated using four layered discontinuous sucrose flotation technique (0.5, 0.75, 1, and 1.5 M) and singlelayered sucrose solution (0.85 M). The isolated cysts were then subjected to DNA extraction by four methods. To remove the artifacts, the extracted DNA were evaluated by PCR. The results of the present study showed the high level of optical density (OD) in the method I (P \ 0.01) with the following steps; Giardia cysts plus crushed cover glass were vortexed. Then, the samples were boiled and then followed by freeze-thaw cycles, yet this method yielded the lowest concentration. Furthermore, the highest concentration were observed in the method II (P \0.01) with the following steps; Giardia cysts plus crushed cover glass and TAE buffer were mixed and then shaken, followed by boiling. Based on the results of the present study, using crushed cover glass, boiling and freeze-thaw cycles can be effective in destruction of G. duodenalis cyst wall and have enough efficiency for extracting DNA from G. duodenalis cysts.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparison and evaluation of four methods for extracting DNA from Giardia duodenalis cysts for PCR targeting the tpi gene

et al. 2016

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“…Abbaszadegan [106] et al reported that this test could detect CRC related genetic alterations by analyzing stool DNA with a sensitivity of 64% and 20% and a specificity of 95% and 100% for long DNA and p16 respectively. An abnormal stool DNA test correlated with a colonoscopically demonstrable abnormality in 49% of cases (34 of 69).…”

Section: Derivative Technique For Crc Screeningmentioning

confidence: 99%

Colonography by CT, MRI and PET/CT combined with conventional colonoscopy in colorectal cancer screening and staging

Sun

Wu²,

Guan³

2008

WJG

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Colorectal cancer (CRC) remains a leading cancer killer worldwide. But the disease is both curable and preventable at an early stage. Regular CRC cancer screening has been shown to reduce the risk of dying from CRC. However, the importance of large-scale screening is only now starting to be appreciated. This article reviews a variety of imaging procedures available for detecting ulcerative colitis (UC) and Crohn's disease (CD), polyps and CRC in their early stage and also presents details on various screening options. Detecting, staging and re-staging of patients with CRC also require multimodality, multistep imaging approaches. Staging and re-staging with conventional colonoscopy (CC), computer tomography colonography (CTC), magnetic resonance colonography (MRC) and positron emission tomography/ c o m p u t e r t o m o g ra p hy c o l o n o g ra p hy ( P E T / C TC ) are of paramount importance in determining the most appropriate therapeutic method and in predicting the risk of tumor recurrence and overall prognosis. The advantages and limitations of these modalities are also discussed.

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“…Furthermore, the DNA recovered from the CRC patients stool was of high molecular weight, unlike fragmented apoptotic DNA recovered from the stool samples of colonoscopy negative individuals (Boynton et al, 2003). Increased amount of high molecular weight DNA (often referred as long DNA or L-DNA) in the stool of CRC patients may result from the decreased apoptosis of the intestinal epithelial cells and/or increased exofoliation of cancerous cells from the tumor(s) (Boynton et al, 2003;Abbaszadegan et al, 2007). Therefore, the differential presence of L-DNA in the stools of CRC patients may be used as a diagnostic marker for CRC (Boynton et al, 2003;Abbaszadegan et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Increased amount of high molecular weight DNA (often referred as long DNA or L-DNA) in the stool of CRC patients may result from the decreased apoptosis of the intestinal epithelial cells and/or increased exofoliation of cancerous cells from the tumor(s) (Boynton et al, 2003;Abbaszadegan et al, 2007). Therefore, the differential presence of L-DNA in the stools of CRC patients may be used as a diagnostic marker for CRC (Boynton et al, 2003;Abbaszadegan et al, 2007). The presence of the L-DNA can easily and reliably be detected by polymerase chain reaction (PCR), making this a sensitive, specific, and affordable CRC test for mass screening.…”

Section: Introductionmentioning

confidence: 99%

Pilot Study of the Sensitivity and Specificity of the DNA Integrity Assay for Stool-based Detection of Colorectal Cancer in Malaysian Patients

Yehya

Yusoff²,

Khalid³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Background: To assess the diagnostic potential of tumor-associated high molecular weight DNA in stool samples of 32 colorectal cancer (CRC) patients compared to 32 healthy Malaysian volunteers by means of polymerase chain reaction (PCR). Methods: Stool DNA was isolated and tumor-associated high molecular weight DNA (1.476 kb fragment including exons 6-9 of the p53 gene) was amplified using PCR and visualized on ethidium bromide-stained agarose gels. Results: Out of 32 CRC patients, 18 were positive for the presence of high molecular weight DNA as compared to none of the healthy individuals, resulting in an overall sensitivity of 56.3% with 100% specificity. Out of 32 patients, 23 had tumor on the left side and 9 on the right side, 16 and 2 being respectively positive. This showed that high molecular weight DNA was significantly (p = 0.022) more detectable in patients with left side tumor (69.6% vs 22.2%). Out of 32 patients, 22 had tumors larger than 1.0 cm, 18 of these (81.8%) being positive for long DNA as compared to not a single patient with tumor size smaller than 1.0 cm (p <0.001). Conclusion: We detected CRC-related high molecular weight p53 DNA in stool samples of CRC patients with an overall sensitivity of 56.3% with 100% specificity, with a strong tumor size dependence.

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Stool-based DNA testing, a new noninvasive method for colorectal cancer screening, the first report from Iran

Cited by 39 publications

References 23 publications

Comparison and evaluation of four methods for extracting DNA from Giardia duodenalis cysts for PCR targeting the tpi gene

Comparison and evaluation of four methods for extracting DNA from Giardia duodenalis cysts for PCR targeting the tpi gene

Colonography by CT, MRI and PET/CT combined with conventional colonoscopy in colorectal cancer screening and staging

Pilot Study of the Sensitivity and Specificity of the DNA Integrity Assay for Stool-based Detection of Colorectal Cancer in Malaysian Patients

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