Stomatocytic or discoidal erythrocyte ghosts containing only spectrin

Nakao, Makoto; Kojima, Yuko; Sato, Shingo; Hara, Yukichi; Wake, Kanako

doi:10.1016/0006-291x(90)91173-p

Cited by 1 publication

(2 citation statements)

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“…Triton-treated ghosts from spherocytes were spherical, and ghosts from elliptocytes were elliptocytic. Moreover, after 2 mol/L sodium iodide treatment, most phospholipids were eliminated, but the appearance was similar to that of the original stomatocytic cells [39]. This organization consisted essentially of spectrin only, and was observed under the electron microscope to be bundled spectrins, although it is not known whether this spectrin organization is the same as that of the native spectrinactin network.…”

Section: Protein Framework: Spectrin-actin Networkmentioning

confidence: 82%

“…As Takakuwa et al [40] indicated that 4.1R deficient cell ghosts were stabilized by the addition of 4.1R The mechanical stability of this spectrin organization, too, was increased by the addition of actin or 4.1R. When both actin and 4.1R were added, the ternary complex was far more stable [39]. Other accessory proteins, such as tropomyosins, tropomodulin, adducin, calmodulin, and so forth, might increase or decrease the stability.…”

Section: Protein Framework: Spectrin-actin Networkmentioning

confidence: 94%

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New insights into regulation of erythrocyte shape

Nakao

2002

Current Opinion in Hematology

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Two ideas explain the mechanism of shape regulation: the lipid bilayer coupled theory and the protein network scaffold theory. Recently, several important articles have been published on the former theory. However, many phenomena argue against the theory, including behavior of ghosts and triton shells, various types of manipulation of proteins, and fixation of the shape by the addition of large reagents outside the cell. Moreover, hereditary spherocytosis shows normal, uneven distribution of phospholipids, and hereditary and artificial defects of a membrane protein show spherocytes or elliptocytes. The liquid state of the lipid layer does not seem to support the shape or mechanical characteristics. On the other hand, all of these phenomena argue for the protein network scaffold theory. Characteristics of each protein and interactions among proteins are now being clarified, but this theory and the author's own ideas still lack decisive evidence.

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Section: Protein Framework: Spectrin-actin Networkmentioning

confidence: 82%

Section: Protein Framework: Spectrin-actin Networkmentioning

confidence: 94%