Stomatin-like Protein-2 Promotes Aggregation, Colonization and Migration of Endometriotic Cells

Kumari, Suparna; Sankhwar, Pushplata; Tripathi, Rupal; Kawale, Ajay K; Gupta, Satish C.; Jha, Rajesh Kumar

doi:10.1007/s43032-022-01139-y

Cited by 1 publication

(1 citation statement)

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“…SDS‐PAGE were run on a tetra protean cell vertical electrophoretic system (BioRad Pvt. India Ltd.) in a running buffer containing 0.10% SDS, 247 mMTris, pH 8.3, 192 mM glycine at 100 V, and then electro‐blotted onto PVDF membrane using transfer buffer containing 20% v/v methanol, 247 mM Tris, pH 8.2, 187 mM glycine at 50 mA for 12 h (Kumari et al, 2022; Towbin et al, 1979).…”

Section: Methodsmentioning

confidence: 99%

Naringenin improves ovarian health by reducing the serum androgen and eliminating follicular cysts in letrozole‐induced polycystic ovary syndrome in the Sprague Dawley rats

Rashid,

Tripathi,

Singh

et al. 2023

Phytotherapy Research

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Polycystic ovary syndrome (PCOS) is most common in women of reproductive age, giving rise to androgen excess and anovulation, leading to infertility and non‐reproductive complications. We explored the ameliorating effect of naringenin in PCOS using the Sprague Dawley (SD) rat model and human granulosa cells. Letrozole‐induced PCOS rats were given either naringenin (50 mg/kg/day) alone or in combination with metformin (300 mg/kg/day), followed by the estrous cycle, hormonal analysis, and glucose sensitivity test. To evaluate the effect of naringenin on granulosa cell (hGC) steroidogenesis, we treated cells with naringenin (2.5 μM) alone or in combination with metformin (1 mM) in the presence of forskolin (10 μM). To determine the steroidogenesis of CYP‐17A1, ‐19A1, and 3βHSD2, the protein expression levels were examined. Treatment with naringenin in the PCOS animal groups increased ovulation potential and decreased cystic follicles and levels of androgens. The expression levels of CYP‐17A1, ‐19A1, and 3βHSD2, were seen restored in the ovary of PCOS SD rats' model and in the human ovarian cells in response to the naringenin. We found an increased expression level of phosphorylated‐AKT in the ovary and hGCs by naringenin. Naringenin improves ovulation and suppress androgens and cystic follicles, involving AKT activation.

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Section: Methodsmentioning

confidence: 99%