Abstract:We propose a continuous Stokes imaging system with a refresh rate of several seconds, instead of a traditional Mueller measurement setup, to quickly track the microstructural changes of tissues during the optical clearing process. The effectiveness of this fast Stokes imaging applied in monitoring the dynamic process is first validated by three designed experiments with a polarization state that changes continuously and rapidly, and is further confirmed by gradual changes in polarization image contrast and res… Show more
“…At the same time, the polarized light path need not be designed separately, as long as the current dynamic light scattering light path-polarizing element can be added. At present, it has been applied to biomedicine ( Xiang-Yu et al, 2019 ; He et al, 2021 ; Meng et al, 2021 ; Shen et al, 2021 ; Song et al, 2021 ), marine microbial detection ( Wang et al, 2018 ; Li et al, 2020; Liu et al, 2020 ; Wang et al, 2020 ; Li et al, 2021 ), atmospheric detection ( Li et al, 2019 ; Qizhi et al, 2021 ), etc. It is believed that with the introduction of polarized light technology, the accuracy, measurable parameters, and applicable range of the dynamic light scattering method will be greatly improved.…”
Section: Online Detection Of Nanoparticlesmentioning
The detection of nanoparticles is the basis of the study of synthesis mechanism, active regulation of the synthesis process, and the study of nanoparticle properties after synthesis. It is significantly meaningful to the academia and engineering industry. Although there are many relevant detection methods at present, each method has its own advantages and disadvantages, and their measurement quantity and application conditions are also different. There is a lack of unified sorting and generalization. In this paper, the significance of detection of nanoparticles synthesized by a gas-phase method is introduced, the development of detection technology is reviewed, and the future is prospected. It is hoped that this paper will provide a reference for the detection of nanoparticles under various conditions and for the development of new detection methods.
“…At the same time, the polarized light path need not be designed separately, as long as the current dynamic light scattering light path-polarizing element can be added. At present, it has been applied to biomedicine ( Xiang-Yu et al, 2019 ; He et al, 2021 ; Meng et al, 2021 ; Shen et al, 2021 ; Song et al, 2021 ), marine microbial detection ( Wang et al, 2018 ; Li et al, 2020; Liu et al, 2020 ; Wang et al, 2020 ; Li et al, 2021 ), atmospheric detection ( Li et al, 2019 ; Qizhi et al, 2021 ), etc. It is believed that with the introduction of polarized light technology, the accuracy, measurable parameters, and applicable range of the dynamic light scattering method will be greatly improved.…”
Section: Online Detection Of Nanoparticlesmentioning
The detection of nanoparticles is the basis of the study of synthesis mechanism, active regulation of the synthesis process, and the study of nanoparticle properties after synthesis. It is significantly meaningful to the academia and engineering industry. Although there are many relevant detection methods at present, each method has its own advantages and disadvantages, and their measurement quantity and application conditions are also different. There is a lack of unified sorting and generalization. In this paper, the significance of detection of nanoparticles synthesized by a gas-phase method is introduced, the development of detection technology is reviewed, and the future is prospected. It is hoped that this paper will provide a reference for the detection of nanoparticles under various conditions and for the development of new detection methods.
“…Our previous work has demonstrated that Mueller matrix and polarization parameters can be used as effective tools to study the microstructural variation due to interaction between clearing agents and biological tissues [28,29]. The elements of MM contain abundant polarization information of tissue.…”
Polarization imaging can quantitatively probe the microscopic structure of biological tissues which can be complex and consist of layered structures. In this paper, we established a fast-backscattering Mueller matrix imaging system to characterize the dynamic variation in the microstructure of single-layer and double-layer tissues as glycerin solution penetrated into the samples. The characteristic response of Mueller matrix elements, as well as polarization parameters with clearer physics meanings, show that polarization imaging can capture the dynamic variation in the layered microstructure. The experimental results are confirmed by Monte Carlo simulations. Further examination on the accuracy of Mueller matrix measurements also shows that much faster speed has to be considered when backscattering Mueller matrix imaging is applied to living samples.
“…The polarization parameters derived from Mueller matrix by Mueller matrix polar decomposition (MMPD) can characterize features of tissues, such as the scattering coefficient, fiber structures, and anisotropy [5,6]. With the increase in the speed of polarization imaging, polarization imaging was gradually being used to study dynamic samples, not only static samples [7][8][9]. Temporal polarization images can increase the information of time dimension, and it has the potential to characterize samples with different microstructures.…”
Polarization is a non-invasive optical technique and has become an emerging new tool in biomedicine studies due to its sensitivity to microstructure. The development of fast polarization techniques has enabled the application of polarimetry not only include static samples such as pathological sections, but also contain dynamic samples such as the process of tissue optical clearing (TOC). Dehydration is one of the important mechanisms of TOC which can result in the changes in tissue anisotropy, samples with different structures will show different characteristics in dehydration. In this study, three kinds of tissues with different degree of anisotropic including skeletal muscle, skin and fat are employed. We extracted and compared the temporal polarization parameters of several samples which may be related to the properties of microstructures. Monte Carlo (MC) simulation was combined to further explore the relationship between temporal polarization parameters and anisotropy of the sample. We first verified the main mechanisms of dehydration in spherecylinder birefringence model (SCBM). Then the optical properties of the microstructure in the model were changed to explore their influence on the polarization parameters. By analyzing the polarization parameters extracted from temporal Stokes images and Monte Carlo simulation, we can present polarization parameters to characterize microstructures of biological tissues.
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