Stoichioproteomics study of differentially expressed proteins and pathways in head and neck cancer

Abstract

Hypoxia is a prominent feature of head and neck cancer. However, the oxygen element characteristics of proteins and how they adapt to hypoxia microenvironments of head and neck cancer are still unknown. Human genome sequences and proteins expressed data of head and neck cancer were retrieved from pathology atlas of Human Protein Atlas project. Then compared the oxygen and carbon element contents between proteomes of head and neck cancer and normal oral mucosa-squamous epithelial cells, genome locations, pathways, and functional dissection associated with head and neck cancer were also studied. A total of 902 differentially expressed proteins were observed where the average oxygen content is higher than that of the lowly expressed proteins in head and neck cancer proteins. Further, the average oxygen content of the up regulated proteins was 2.54% higher than other. None of their coding genes were distributed on the Y chromosome. The up regulated proteins were enriched in endocytosis, apoptosis and regulation of actin cytoskeleton. The increased oxygen contents of the highly expressed and the up regulated proteins might be caused by frequent activity of cytoskeleton and adapted to the rapid growth and fast division of the head and neck cancer cells. The oxygen usage bias and key proteins may help us to understand the mechanisms behind head and neck cancer in targeted therapy, which lays a foundation for the application of stoichioproteomics in targeted therapy and provides promise for potential treatments for head and neck cancer.

Z-Ligustilide Induces c-Myc-Dependent Apoptosis via Activation of ER-Stress Signaling in Hypoxic Oral Cancer Cells

“…The latest research suggests that ligustilide may inhibit the activation and apoptosis of cancer-associated fibroblasts (CAFs) in the tumor microenvironment, thereby altering the immunosuppressive function of CAFs ( 20 ). In addition, no research to date has suggested that ligustilide had anti-cancer effects in oral cancer cells, but it is known that a hypoxic microenvironment is a significant feature of oral cancer ( 21 ). Therefore, this study aims to explore the anti-cancer activity and related molecular mechanisms of ligustilide on hypoxic oral cancer cells.…”