Stoffwechselphysiologische Untersuchungen an einem kohlenwasserstoffoxydierenden Pseudomonas-Stamm aus der Elbe

Schöberl, Peter

doi:10.1007/bf00425210

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…2: it appears that resting cells of C. lipolytica metabolized cetylalcohol, palniitaldehj-de and palmitic wit1 without lap: not only when the yeast, 1966. andSCHOBERL 1967).…”

Section: )mentioning

confidence: 99%

Adaptative or constitutive nature of the enzymes involved in the oxidation of n‐hexadecane into palmitic acid by Candida lipolytica

Nyns

Auquière

Wiaux

1969

J of Basic Microbiology

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Yeasts have been known to giow at the expense of n-paraffins for a number of years (TAUSSON 1939). It is generally accepted that the alkane is mainly metabolized through the corresponding primary alcohol, aldehyde and fatty acid. The enzymes involved in this pathway are adaptative or constitutive depending upon the microorganism studied (VAN DER LINDEN and THIJSSE 1956).Candida lipolytica is one of the most extensively investigated hydrocarbon assimilating microorganisms. Whether the enzymes, by which the yeast oxidizes n-hexadecane into palmitic acid, are constitutive to the microbial cell or formed adaptatively upon exposure to the substrate, has remained open to question, and was investigated by respirometric experiments in a WARBURG apparatus using resting cells of C. lipolytica grown on 1 yo n-hexadecane, cetylalcohol, palmitaldehyde, palmitic acid or glucose. 1969 373-380 JIuterial and methodsCultures: Candida Zipolytica, strain 599 from the Centraal Bureau voor Schimmelkulturen (C. B. S., Delft, Neth.) was maintained, grown on either 1% n-hexadecane or glucose, and harvested in the middle of its logarithmic phase of growth, as described earlier (NYNS et al. 1967). The same procedure was used when cetylalcohol, palmitaldehyde or palmitic acid served as sole source of carbon and energy. I n these cases, 100 ml of the liquid growth medium were prepared as follows: 1 g of the carbon source and 89 ml distilled water were steam-sterilized a t 120 "C for 20 min. A tenfold concentrated aqueous solution of Yeast Nitrogen Base (DIFCO) was filter-sterilized through a membrane of 0.22 p diameter (Millipore, Bedford, Mass.). 10 ml of this solution were aseptically added to the sterilized medium.The shaft of an Ultra-Turrax (type TP 18/2, JANKE and KUNKEL, Staufen i. Br., Germ.) was sterilized with ethanol and rinsed with sterile distilled water. The growth medium was aseptically homogenized a t top speed (12,000 rpm) for 1 min. The microbial population was estimated by counting appropriate dilutions of the cell suspension using a THOMAS haemacytometer. Cell clusters were homogenized with Tween 80 (NYKS et al. 1967).The cell crop was washed and the dry weight of the washed cell suspension was determined as reported earlier (NYNS et al. 1968). FRITSCHE (1966) could not use C. Zipolytica grown on n-alkanes for WARBURG respirometric studies, because the endogenous respiration was too high. ROBINSON (1964) reduced the endogenous respiration by starvation in distilled water for 2 hr. a t room temperature. In the case of C . lipolytica, it was necessary t o incubate the yeast cells in a carbonfree nutrient medium for 24 hr. before the endogenous respiration substantially decreased (NYNS et UZ. 1968). Whenever n-hexadecane, cetylalcohol, palmitaldehyde or palmitic acid served as the carbon source, the cells were starved in this manner. Before starvation, hydrocarbon grown cells contained up to 1/3 of their weight of adsorbed

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“…2: it appears that resting cells of C. lipolytica metabolized cetylalcohol, palniitaldehj-de and palmitic wit1 without lap: not only when the yeast, 1966. andSCHOBERL 1967).…”

Section: )mentioning

confidence: 99%

Adaptative or constitutive nature of the enzymes involved in the oxidation of n‐hexadecane into palmitic acid by Candida lipolytica

Nyns

Auquière

Wiaux

1969

J of Basic Microbiology

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“…Die Wechselwirkungen des Komplexes Wasser-MineralOl-Mikroorganismen waren seit der Jahrhundertwende immer wieder Gegenstand yon Untersuchungen; die Resultate einiger hundert Originalarbeiten sind in Sammelpublikationen yon Zobell [50], Fuhs [13], Scb0berl [34,35], Beerstecher [3], Davis [8] und Davis [9] zusammengefasst. Als System wurde die Bakterienflora in ungestOrtem Seesediment gewahlt, als Belastungsparameter HeizOl ((extra leicht)).…”

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Der Einfluss von Kohlenwasserstoffen auf die Ökologie der Bakterien im aeroben Seesediment

Bührer¹

1979

Schweiz. Z. Hydrologie

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Influence of hydrocarbons on ecology of bacteria in aerobic lake sedimentTwo different hydrocarbon loadings of sediment cores were analyzed 4 times during 21 days. Applied methods were: Direct count, isolation of more than 100 colonies on a diluted plate-count agar and estimation of 42 attributes (282 levels), followed by a single link clustering and calculation of the information basing on attribute level (i.e. negative entropy). Loading of 100 g fuel oil/m 2. sediment caused a depletion of germs and information. Full restoration could not be observed. When 10 g fuel/m 2 was applied there was no significant effect, but if algae were supplied too, the effect was similar to using 100 g/m 2. An extrapolation of the results gives a tolerance of hydrocarbon loading for Lake Constance or Lake Zug of 10 to 20 times the load present, but effects on fishes or fishfood were not considered. 1) Die Arbeit wurde an der Eidgen~ssischen Anstalt Far Wasserversorgung, Abwasserreinigung und Gewasserschutz (EAWAG) in D@bendorf ausgefOhrt. Kurzfassung ETH Diss. Nr.6121.

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Adaptative or constitutive nature of the enzymes involved in the oxidation ofn-hexadecane into palmitic acid byCandida lipolytica

Nyns¹,

Auquière²,

Wiaux³

1969

Z Allg Mikrobiol

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Stoffwechselphysiologische Untersuchungen an einem kohlenwasserstoffoxydierenden Pseudomonas-Stamm aus der Elbe

Cited by 6 publications

References 18 publications

Adaptative or constitutive nature of the enzymes involved in the oxidation of n‐hexadecane into palmitic acid by Candida lipolytica

Adaptative or constitutive nature of the enzymes involved in the oxidation of n‐hexadecane into palmitic acid by Candida lipolytica

Der Einfluss von Kohlenwasserstoffen auf die Ökologie der Bakterien im aeroben Seesediment

Adaptative or constitutive nature of the enzymes involved in the oxidation ofn-hexadecane into palmitic acid byCandida lipolytica

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