Stochastic electrotransport selectively enhances the transport of highly electromobile molecules

Kim, Sung-Yon; Cho, Jaehun; Murray, Evan; Bakh, N.A.; Choi, Heejin; Ohn, Kimberly; Ruelas, Luzdary; Hubbert, Austin; McCue, Meg; Vassallo, Sara; Keller, Philipp J.; Chung, Kwanghun

doi:10.1073/pnas.1510133112

Cited by 209 publications

(249 citation statements)

References 44 publications

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“…15 However, strong electric field or high temperatures can damage tissue-gel hybrids on both a structural and molecular level. Stochastic implementations of electrophoresis 16 and novel tissue-hybrid recipes 17 improve sample stability while keeping lipid removal time quite short (few days). Continuous perfusion of the sample with SDS is another approach to improve clearing speed.…”

Section: Tissue Transformationmentioning

confidence: 99%

“…Recently, a speed-up of the clearing process has been achieved using stochastic electrotransport of lipids. 16 The use of electrophoresis, either "standard" or stochastic, introduces great technical complexity, unless a commercial system is used. Passive methods, relying on spontaneous diffusion of lipids, have been described.…”

Section: Endogenous Fluorescencementioning

confidence: 99%

“…5,6,[8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27] Here, we try to classify clearing approaches based on the microscopy technique where they can be applied, rather than on the clearing mechanism. 7 We believe that this new taxonomy can be a helpful starting point for microscopists willing to work with transparent tissue.…”

Section: Introductionmentioning

confidence: 99%

“…16,17 Tissue-transforming methods are usually quite complex and expensive; nonetheless, they are attractive because of the high degree of transparency and of the increased sample porosity, which helps whole-mount IHC on large portions of tissue. 14,19 Diffusion of antibody inside the sample can be further facilitated by the application of stochastic electric fields.…”

mentioning

confidence: 99%

“…14,19 Diffusion of antibody inside the sample can be further facilitated by the application of stochastic electric fields. 16 …”

mentioning

confidence: 99%

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Clearing of fixed tissue: a review from a microscopist’s perspective

Costantini²,

et al. 2016

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Abstract. Chemical clearing of fixed tissues is becoming a key instrument for the three-dimensional reconstruction of macroscopic tissue portions, including entire organs. Indeed, the growing interest in this field has both triggered and been stimulated by recent advances in high-throughput microscopy and data analysis methods, which allowed imaging and management of large samples. The strong entanglement between clearing methods and imaging technology is often overlooked, as typical classification of the former is based only on the chemicals used. Here, we review the recent literature in the field, proposing a taxonomy of clearing techniques based on their mating with the major high-throughput microscopies. We hope that this application-oriented classification can help researchers to find the protocol best suited to their experiment among the many present in the literature.

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Section: Tissue Transformationmentioning

confidence: 99%

Section: Endogenous Fluorescencementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

“…14,19 Diffusion of antibody inside the sample can be further facilitated by the application of stochastic electric fields. 16 …”

mentioning

confidence: 99%

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Clearing of fixed tissue: a review from a microscopist’s perspective

Costantini²,

et al. 2016

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Tissue‐Engineered Peripheral Nerve Interfaces

Spearman

Desai

Mobini

et al. 2017

Adv Funct Materials

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Research on neural interfaces has historically concentrated on development of systems for the brain; however, there is increasing interest in peripheral nerve interfaces (PNIs) that could provide benefit when peripheral nerve function is compromised, such as for amputees. Efforts focus on designing scalable and high-performance sensory and motor peripheral nervous system interfaces. Current PNIs face several design challenges such as undersampling of signals from the thousands of axons, nerve-fiber selectivity, and device-tissue integration. To improve PNIs, several researchers have turned to tissue engineering. Peripheral nerve tissue engineering has focused on designing regeneration scaffolds that mimic normal nerve extracellular matrix composition, provide advanced microarchitecture to stimulate cell migration, and have mechanical properties like native nerve. By combining PNIs with tissue engineering, the goal is to promote natural axon regeneration into the devices to facilitate close contact with electrodes; in contrast, traditional PNIs rely on insertion or placement of electrodes into or around existing nerve, or do not utilize materials to actively facilitate axon regeneration. This review presents the state-of-the-art of PNIs and nerve tissue engineering, highlights recent approaches to combine neural interface technology and tissue engineering, and addresses remaining challenges with foreign body response.

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MACS: Rapid Aqueous Clearing System for 3D Mapping of Intact Organs

Zhu

et al. 2020

Advanced Science

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Tissue optical clearing techniques have provided important tools for large‐volume imaging. Aqueous‐based clearing methods are known for good fluorescence preservation and scalable size maintenance, but are limited by long incubation time, insufficient clearing performance, or requirements for specialized devices. Additionally, few clearing methods are compatible with widely used lipophilic dyes while maintaining high clearing performance. Here, to address these issues, m‐xylylenediamine (MXDA) is firstly introduced into tissue clearing and used to develop a rapid, highly efficient aqueous clearing method with robust lipophilic dyes compatibility, termed MXDA‐based Aqueous Clearing System (MACS). MACS can render whole adult brains highly transparent within 2.5 days and is also applicable for other intact organs. Meanwhile, MACS possesses ideal compatibility with multiple probes, especially for lipophilic dyes. MACS achieves 3D imaging of the intact neural structures labeled by various techniques. Combining MACS with DiI labeling, MACS allows reconstruction of the detailed vascular structures of various organs and generates 3D pathology of glomeruli tufts in healthy and diabetic kidneys. Therefore, MACS provides a useful method for 3D mapping of intact tissues and is expected to facilitate morphological, physiological, and pathological studies of various organs.

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Stochastic electrotransport selectively enhances the transport of highly electromobile molecules

Cited by 209 publications

References 44 publications

Clearing of fixed tissue: a review from a microscopist’s perspective

Clearing of fixed tissue: a review from a microscopist’s perspective

Tissue‐Engineered Peripheral Nerve Interfaces

MACS: Rapid Aqueous Clearing System for 3D Mapping of Intact Organs

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