STMN1 as a novel prognostic biomarker in HCC correlating with immune infiltrates and methylation

Zhang, En-di; Li, Chenxuan; Fang, Yuan; Li, Na; Xiao, Zhongyun; Chen, Chuhong; Wei, Benkai; Wang, Hangping; Xie, Jin‐Cheng; Miao, Yinghui; Zeng, Zhong; Huang, Hanfei

doi:10.1186/s12957-022-02768-y

Cited by 12 publications

(7 citation statements)

References 35 publications

(43 reference statements)

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“…In addition, the expression of Stathmin 1 is closely related to immune cell infiltration, including CD8+T cells, CD4+T cells, B cells, TINs, macrophages, and monocytes [ 13 ]. Zhang ED et al [ 23 ] find that the STMN1 expression is associated with immune cell infiltration, immune molecules, and immune checkpoints in hepatocellular carcinoma. In this study, the number of TINs infiltration in cervical tissue was strongly higher in the Stathmin positive group than that in the negative group, and the contents of CD4+T and CD8+T was lower than those in the control group.…”

Section: Discussionmentioning

confidence: 99%

The relationship between the expression of Stathmin and tumor immune cell infiltration in primary cervical carcinoma and its prognostic diagnostic value

Yang,

Li,

Tang

et al. 2023

Heliyon

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Section: Discussionmentioning

confidence: 99%

The relationship between the expression of Stathmin and tumor immune cell infiltration in primary cervical carcinoma and its prognostic diagnostic value

Yang,

Li,

Tang

et al. 2023

Heliyon

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“…STMN1 is a cytosolic phosphoprotein that regulates microtubule dynamics in response to cellular needs, and plays an important role in mitotic spindle formation and cell division (Rubin and Atweh 2004 ; Hu et al 2020 ). The methylation of STMN1 is associated with the prognosis of HCC, and the expression of STMN1 is closely related to the change of m6A (Zhang et al 2022 ). CLSPN, as the gene encoding Claspin protein, plays an important role in key cellular events such as checkpoint activation after DNA damage, DNA replication and replication stress response, DNA repair and apoptosis (Azenha et al 2017 , 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of CPSF4-related alternative splice genes in hepatocellular carcinoma

Yuemaierabola,

Guo,

Sun

et al. 2023

J Cancer Res Clin Oncol

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Background An important stage in controlling gene expression is RNA alternative splicing (AS), and aberrant AS can trigger the development and spread of malignancies, including hepatocellular carcinoma (HCC). A crucial component of AS is cleavage and polyadenylation-specific factor 4 (CPSF4), a component of the CPSF complex, but it is unclear how CPSF4-related AS molecules describe immune cell infiltration in the total tumor microenvironment (TME). Methods Using RNA-sequencing data and clinical data from TCGA-LIHC from the Cancer Genome Atlas (TCGA) database, the AS genes with differential expression were found. The univariate Cox analysis, KM analysis, and Spearman analysis were used to identify the AS genes related to prognosis. Screening of key AS genes that are highly correlated with CPSF4. Key genes were screened using Cox regression analysis and stepwise regression analysis, and prognosis prediction models and the topography of TME cell infiltration were thoroughly analyzed. Results A model consisting of seven AS genes (STMN1, CLSPN, MDK, RNFT2, PRR11, RNF157, GHR) was constructed that was aimed to predict prognostic condition. The outcomes of the HCC samples in the high-risk group were considerably worse than those in the lower risk group (p < 0.0001), and different risk patient groups were formed. According to the calibration curves and the area under the ROC curve (AUC) values for survival at 1, 2, and 3 years, the clinical nomogram performs well in predicting survival in HCC patients. These values were 0.76, 0.70, and 0.69, respectively. Moreover, prognostic signature was markedly related to immune infiltration and immune checkpoint genes expression. Conclusion By shedding light on the function of CPSF4 and the seven AS genes in the formation and progression of HCC, this research analysis contributes to the development of more useful prognostic, diagnostic, and possibly therapeutic biomarkers.

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“…In addition, STMN1 is upregulated in HCC, which is linked to clinicopathological parameters and influences prognosis in HCC patients. 28 The poor expression of CBS has been reported in HCC, which predicts the poor prognosis of HCC patients. 29 Likewise, the inactivation of the angiogenesis inhibitor EGLN2 is correlated with the poor prognosis of HCC.…”

Section: Discussionmentioning

confidence: 99%

CENPA-driven STMN1 Transcription Inhibits Ferroptosis in Hepatocellular Carcinoma

Liang¹,

Luo²,

Wang³

et al. 2023

J Clin Transl Hepatol

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Background and Aims The growing knowledge of ferroptosis has suggested the regulatory role of ferroptosis in hepatocellular carcinoma (HCC), but the pertinent molecular mechanisms remain unclear. Herein, this study investigated the mechanistic basis of ferroptosis-related genes (ferrGenes) in the growth of HCC. Methods Differentially expressed human ferrGenes and tumor-related transcription factors (TFs) were obtained from the The Cancer Genome Atlas (TCGA) dataset and the GTEx dataset. Spearman method-based correlation analysis were conducted to construct TF-ferrGene coexpression regulatory network. Key genes associated with prognosis were singled out with Lasso regression and multivariate Cox analysis to construct the prognostic risk model. Then the accuracy and independent prognostic ability of the model were evaluated. Expression of CENPA and STMN1 was determined in clinical HCC tissues and HCC cells, and their binding was analyzed with dual-luciferase and chromatin immunoprecipitation (ChIP) assays. Furthermore, ectopic expression and knockdown assays were performed in HCC cells to assess the effect of CENPA and STMN1 on ferroptosis and malignant phenotypes. Results The prognostic risk model constructed based on the eight TF-ferrGene regulatory network-related genes accurately predicted the prognosis of HCC patients. It was strongly related to the clinical characteristics of HCC patients. Moreover, CENPA/STMN1 might be a key TF-ferrGene regulatory network in ferroptosis of HCC. CENPA and STMN1 were overexpressed in HCC tissues and cells. Additionally, CENPA facilitated STMN1 transcription by binding to STMN1 promoter, thus facilitating the malignant phenotypes and suppressing the ferroptosis of HCC cells. Conclusions Taken together, CENPA curbs the ferroptosis of HCC cells by upregulating STMN1 transcription, thereby promoting HCC growth.

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STMN1 as a novel prognostic biomarker in HCC correlating with immune infiltrates and methylation

Cited by 12 publications

References 35 publications

The relationship between the expression of Stathmin and tumor immune cell infiltration in primary cervical carcinoma and its prognostic diagnostic value

The relationship between the expression of Stathmin and tumor immune cell infiltration in primary cervical carcinoma and its prognostic diagnostic value

Comprehensive analysis of CPSF4-related alternative splice genes in hepatocellular carcinoma

CENPA-driven STMN1 Transcription Inhibits Ferroptosis in Hepatocellular Carcinoma

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