Stimulus-transcription coupling in neurons: role of cellular immediate-early genes

Morgan, James I.; Curran, Tom

doi:10.1016/0166-2236(89)90096-9

Cited by 889 publications

(343 citation statements)

References 39 publications

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“…In order to investigate the neural substrates mediating THC-induced alterations in task performance, we also characterized regional alterations in the expression levels of mRNA encoding two immediate-early genes (IEGs), c-fos and ngfib, as a consequence of both THC administration and discrimination performance. IEG expression provides a marker of alterations in regional neural activation occurring in response to several stimuli (Morgan and Curran, 1989). C-fos and ngfi-b were selected as markers for use in the present study as they belong to complementary transcription factor families (Persico and Uhl, 1996) and because initial studies showed that these IEGs were sensitive to THC administration.…”

Section: Introductionmentioning

confidence: 99%

Acute Δ9-Tetrahydrocannabinol-Induced Deficits in Reversal Learning: Neural Correlates of Affective Inflexibility

Egerton

Brett

Pratt

2005

Neuropsychopharmacol

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Despite concerns surrounding the possible adverse effects of marijuana on complex cognitive function, the processes contributing to the observed cognitive deficits are unclear, as are the causal relationships between these impairments and marijuana exposure. In particular, marijuana-related deficits in cognitive flexibility may affect the social functioning of the individual and may contribute to continued marijuana use. We therefore examined the ability of rats to perform affective and attentional shifts following acute administration of D 9 -tetrahydrocannabinol (THC), the primary psychoactive marijuana constituent. Administration of 1 mg/kg THC produced marked impairments in the ability to reverse previously relevant associations between stimulus features and reward presentation, while the ability to transfer attentional set between dimensional stimulus properties was unaffected. Concurrent in situ hybridization analysis of regional c-fos and ngfi-b expression highlighted areas of the prefrontal cortex and striatum that were recruited in response to both THC administration and task performance. Furthermore, the alterations in mRNA expression in the orbitofrontal cortex and striatum were associated with the ability to perform the reversal discriminations. These findings suggest that marijuana use may produce inelasticity in updating affective associations between stimuli and reinforcement value, and that this effect may arise through dysregulation of orbitofrontal and striatal circuitry.

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Section: Introductionmentioning

confidence: 99%

Acute Δ9-Tetrahydrocannabinol-Induced Deficits in Reversal Learning: Neural Correlates of Affective Inflexibility

Egerton

Brett

Pratt

2005

Neuropsychopharmacol

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“…Findings, which led to the conclusion that the PEA and the DYN genes may be a target for the fos_jrrrtlAP-I complex, which binds to an AP-1 DNA motif. thereby modulating basal and enhanced PEA and DYN mRNA expression [9,26,28]. Based on these observations and the fact that the CCK and PEA gents share structurally related regulatory clements [1,18,22] and Fig.…”

Section: Discussionmentioning

confidence: 99%

Phorbol 12‐myristate‐13‐acetate (PMA) stimulates a differential expression of cholecystokinin (CCK) and c‐fos MRNA in a human neuroblastoma cell line

Monstein

Folkesson

1991

FEBS Letters

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“…Thereafter, mRNA accumulates and reaches peak values at 30-45 min post-stimulation followed by a decline with the relatively short halflife of approximately 12 min [57]. Fos protein is also relatively short-lived, with a half-life between 2 h and 6 h after an acute challenge returning to baseline control before 24 h [42,56], as is typical of an intracellular signaling system. Unlike the N-terminal antibody that we used in this study and that has been shown to be specific for c-fos [76,77], other M peptide antibodies have been shown to recognize epitopes shared between Fos and various Fos-related antigens (FRA).…”

Section: Persistence Of Fos Immunoreactivity In the Ldt/ppt Cell Groupsmentioning

confidence: 99%

“…Expression of c-fos has been used extensively as a cellular marker of neuronal activity [56] and has been of particular interest to behavioral neuroscientists investigating sleep-wake state control [8][9][10][11]47,52,76,81,82,90]. Moreover, alterations in the expression of transactivating proteins in the brain that influence the transcription of genes involved in long-term changes in behavioral phenotype are well established for the protein products of transcription factor activating protein-1 (AP-1) genes, particularly c-fos [36,57,84].…”

Section: Introductionmentioning

confidence: 99%

From synapse to gene product: prolonged expression of c-fos induced by a single microinjection of carbachol in the pontomesencephalic tegmentum

Quattrochi

Bazalakova

Hobson

2005

Molecular Brain Research

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It is not known how the brain modifies its regulatory systems in response to the application of a drug, especially over the long term of weeks and months. We have developed a model system approach to this question by manipulating cholinergic cell groups of the laterodorsal and pedunculopontine tegmental (LDT/PPT) nuclei in the pontomesencephalic tegmentum (PMT), which are known to be actively involved in the timing and quantity of rapid eye movement (REM) sleep. In a freely moving feline model, a single microinjection of the cholinergic agonist carbachol conjugated to a latex nanosphere delivery system into the caudolateral PMT elicits a long-term enhancement of one distinguishing phasic event of REM sleep, ponto-geniculo-occipital (PGO) waves, lasting 5 days but without any significant change in REM sleep or other behavioral state. Here, we test the hypothesis that cholinergic activation within the caudolateral PMT alters the postsynaptic excitability of the PGO network, stimulating the prolonged expression of c-fos that underlies this long-term PGO enhancement (LTPE) effect. Using quantitative Fos immunohistochemistry, we found that the number of Fos-immunoreactive (Fos-IR) neurons surrounding the caudolateral PMT injection site decreased sharply by postcarbachol day 03, while the number of Fos-IR neurons in the more rostral LDT/PPT increased >30-fold and remained at a high level following the course of LTPE. These results demonstrate a sustained c-fos expression in response to pharmacological stimulation of the brain and suggest that carbachol's acute effects induce LTPE via cholinergic receptors, with subsequent transsynaptic activation of the LDT/PPT maintaining the LTPE effect.

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Stimulus-transcription coupling in neurons: role of cellular immediate-early genes

Cited by 889 publications

References 39 publications

Acute Δ9-Tetrahydrocannabinol-Induced Deficits in Reversal Learning: Neural Correlates of Affective Inflexibility

Acute Δ9-Tetrahydrocannabinol-Induced Deficits in Reversal Learning: Neural Correlates of Affective Inflexibility

Phorbol 12‐myristate‐13‐acetate (PMA) stimulates a differential expression of cholecystokinin (CCK) and c‐fos MRNA in a human neuroblastoma cell line

From synapse to gene product: prolonged expression of c-fos induced by a single microinjection of carbachol in the pontomesencephalic tegmentum

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