Stimulus-secretion coupling in angiotensin-stimulated adrenal glomerulosa cells

Spät, András

doi:10.1016/0022-4731(88)90256-7

Cited by 58 publications

(42 citation statements)

References 70 publications

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“…2A, ϩCa) (12). The transient rise in [Ca 2ϩ ] i has been attributed to the release of internal stores, while the sustained levels are a result of influx of extracellular calcium to fill these depleted membrane stores (1,4,42). The maximal [Ca 2ϩ ] i measured is referred to as peak calcium, while the sustained calcium level is reported as the calcium concentration 3-5 min after agonist treatment.…”

Section: Extracellular Calcium Is Required For Star Transcriptionmentioning

confidence: 99%

“…The two major physiological regulators of aldosterone synthesis in the adrenal zona glomerulosa are angiotensin II (Ang II) 1 and extracellular K ϩ (1). Ang II binds to AT1 G-protein-coupled cell surface receptors that activate phospholipase C and results in the release of diacylglycerol and inositol 1,4,5-trisphosphate from plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (2).…”

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confidence: 99%

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Janus Kinase 2 and Calcium Are Required for Angiotensin II-dependent Activation of Steroidogenic Acute Regulatory Protein Transcription in H295R Human Adrenocortical Cells

Feltzer

Dawson

et al. 2003

Journal of Biological Chemistry

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Angiotensin II-and K ؉ -stimulated aldosterone production in the adrenocortical glomerulosa cells requires induction of the steroidogenic acute regulatory protein (StAR). While both agents activate Ca 2؉ signaling, the mechanisms leading to aldosterone synthesis are distinct, and the angiotensin II response cannot be mimicked by K ؉ . We previously reported that StAR mRNA levels and promoter-reporter gene activity in transiently transfected H295R human adrenocortical cells were stimulated by angiotensin II but not by K ؉ treatment. The current study focused on identifying signaling pathways activated by angiotensin II that contribute to StAR transcriptional activation. We show that the angiotensin II-stimulated transcriptional activation of StAR was dependent upon influx of external calcium and requires protein kinase C activation. Furthermore we describe for the first time that the Janus tyrosine kinase family member, JAK2, was activated by angiotensin II treatment of H295R cells. Treatment of the cells with AG490, a selective inhibitor of JAK2, blocked JAK2 activation and StAR reporter gene activity and inhibited steroid production. Taken together these studies describe a novel pathway controlling StAR expression and steroidogenesis in adrenocortical cells.

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Section: Extracellular Calcium Is Required For Star Transcriptionmentioning

confidence: 99%

mentioning

confidence: 99%

Janus Kinase 2 and Calcium Are Required for Angiotensin II-dependent Activation of Steroidogenic Acute Regulatory Protein Transcription in H295R Human Adrenocortical Cells

Feltzer

Dawson

et al. 2003

Journal of Biological Chemistry

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“…The AT1 receptor in the zona glomerulosa signals via the calcium/inositol trisphosphate second messenger pathway (Spät 1988, Catt et al 1988, Rossig et al 1996. However, angiotensin II is also known to stimulate the MAP kinase (MAPK) pathway which may participate in proliferation.…”

Section: Introductionmentioning

confidence: 99%

Distribution of extracellular signal-regulated protein kinases 1 and 2 in the rat adrenal and their activation by angiotensin II

McNeill

Whitworth²,

Vinson³

et al. 2005

Journal of Endocrinology

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The adrenal gland of the rat is continuously regenerated through proliferation of a stem cell population in the outer part of the gland. To clarify the location of proliferative events within the adrenal gland, and the factors that stimulate them, rat adrenal capsule preparations, consisting of capsule, zona glomerulosa (ZG) and the outer zona fasciculata (ZF) were maintained in vitro under different conditions of stimulation, for varying periods. Sites of proliferation were identified by 5-bromo-2 -deoxyuridine (BrdU) staining, and the distribution of classical MAP kinase (MAPK) family members, extracellular signal-regulated kinase (ERK) 1 and 2, immunoreactivity was determined using immunocytochemistry. BrdU staining was limited to the outer glomerulosa and the capsule, where it was enhanced by angiotensin II, but not by a high potassium ion concentration nor by ACTH. In contrast, ERK1/2 immunoreactivity was distributed throughout the ZG and in the medulla, with none detectable in the ZF and reticularis. Furthermore, angiotensin II, potassium ions and ACTH were all shown to induce ERK1 and ERK2 phosphorylation in the ZG. Treatment of adrenal capsule tissue with the specific MAPK kinase inhibitor PD98059 revealed inhibition of ERK1/2 phosphorylation, but no effect on angiotensin II-induced aldosterone secretion. Although the distribution and activation of the MAPK pathway suggest a link with proliferation, the findings clearly designated only the outer part of the glomerulosa and capsule as a potential stem cell population. Further functions should be sought for the apparently silent major part of the glomerulosa.

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“…The cultured skin fibroblasts were used to investigate whether occupancy of fetal AII receptors results in phospholipid breakdown and calcium mobilization (11), as occur in other target tissues (12,13). Endogenous phosphoinositide pools were labeled by preincubation of the cells for 18 hours with [3H]inositol and incubated with AII.…”

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confidence: 99%

Novel Sites of Expression of Functional Angiotensin II Receptors in the Late Gestation Fetus

Millán

Carvallo

Izumi

et al. 1989

Science

155

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In the adult, the peptide hormone angiotensin II (AII) is primarily known as a regulator of circulatory homeostasis, but recent evidence also suggests a role in cell growth. This study of AII in late gestation rat fetuses revealed the unexpected presence of receptors in skeletal muscle and connective tissue, in addition to those in recognized adult target tissues. The AII receptors in this novel location decreased by 80 percent 1 day after birth and were almost undetectable in the adult. Studies in fetal skin fibroblasts showed that the receptors were coupled to phospholipid breakdown, with concomitant increases in inositol phosphate and cytosolic calcium. The abundance, timing of expression, and unique localization of functional AII receptors in the fetus suggest a role for AII in fetal development.

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Stimulus-secretion coupling in angiotensin-stimulated adrenal glomerulosa cells

Cited by 58 publications

References 70 publications

Janus Kinase 2 and Calcium Are Required for Angiotensin II-dependent Activation of Steroidogenic Acute Regulatory Protein Transcription in H295R Human Adrenocortical Cells

Janus Kinase 2 and Calcium Are Required for Angiotensin II-dependent Activation of Steroidogenic Acute Regulatory Protein Transcription in H295R Human Adrenocortical Cells

Distribution of extracellular signal-regulated protein kinases 1 and 2 in the rat adrenal and their activation by angiotensin II

Novel Sites of Expression of Functional Angiotensin II Receptors in the Late Gestation Fetus

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