Stimuli Responsive, Programmable DNA Nanodevices for Biomedical Applications

Singh, Umesh Kumar; Morya, Vinod; Datta, Bhaskar; Ghoroi, Chinmay; Bhatia, Dhiraj

doi:10.3389/fchem.2021.704234

Cited by 13 publications

(11 citation statements)

References 131 publications

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“…If both (or either) of these two processes proceed only upon the applications of these stimuli, therapeutic efficacy of the drug should be greatly promoted, and undesired side-effects on healthy cells are suppressed. [24][25][26][156][157][158] In fact, most of the recent studies on DDS employ stimuli-responsive strategies. In one of the two plausible approaches, intracellular signals which are specific to target cells and tissues are used as the stimulators of DDS.…”

Section: Smart Dds Using Intracellular Signals As Stimuli To Accompli...mentioning

confidence: 99%

“…Other physical factors (e.g., magnetic field and electric field) also have a potential as external stimuli. [18,19,25]…”

Section: Smart Dds Using Physical Stimuli Provided From the Outside O...mentioning

confidence: 99%

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Among these materials, DNA (and also RNA) is especially promising for the future applications, because of its remarkably high molecular recognition, low cytotoxicity, sufficient availability, and high biocompatibility. [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] Predetermined functional groups can be introduced to desired site of DNA through appropriate chemical modification. The mechanical rigidity, biological stability, and other physicochemical properties can be also modulated by chemical means.…”

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confidence: 99%

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DNA‐Based Nanoarchitectures as Eminent Vehicles for Smart Drug Delivery Systems

Komiyama

Shigi

Ariga

2022

Adv Funct Materials

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In order to cure diseases efficiently with minimal side effects in sophisticated medicine, eminent drugs must be selectively delivered to diseased parts and allow to work only there. However, most of drug delivery systems (DDSs) previously proposed are not yet sufficiently selective and effective. This review covers recent developments of DDS in which DNA nanoarchitectures are employed as drug carriers. From short DNA fragments, a variety of complicated nanomedicines are fabricated to show unprecedentedly smart DDS functions. Encapsulated drugs are released mostly through controlled disassembly of DNA nanoarchitectures. Recent attention has been focusing to the use of appropriate stimuli to regulate each process in the DDS (delivery of drug, its release, and others) for further improved therapy. As intracellular stimuli for the regulation, various endogenous compounds (e.g., mRNA and enzymes), which are specifically abundant in target cells, are employed. Alternatively, physical perturbation (e.g., light irradiation) is provided from outside of tissues to control DDS. By combining multiple strategies in terms of new concepts, enormously smart DDSs are being achieved. Further progresses of these approaches in the future are guaranteed by complete freedom of molecular design in DNA nanoarchitectonics.

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Section: Smart Dds Using Intracellular Signals As Stimuli To Accompli...mentioning

confidence: 99%

“…Other physical factors (e.g., magnetic field and electric field) also have a potential as external stimuli. [18,19,25]…”

Section: Smart Dds Using Physical Stimuli Provided From the Outside O...mentioning

confidence: 99%

mentioning

confidence: 99%

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DNA‐Based Nanoarchitectures as Eminent Vehicles for Smart Drug Delivery Systems

Komiyama

Shigi

Ariga

2022

Adv Funct Materials

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“…The design of dynamic molecular- and nanomachines and their higher-order interaction networks is a cross-disciplinary research area that has seen tremendous recent growth. − In terms of achieving practical applications, such machines need to be coupled to the outside world, and in particular, need to function effectively in aqueous/biological media. In this regard, dynamic DNA chemistry/nanotechnology has led the way generating controlled dynamic systems with potential therapeutic, diagnostic, and computational applications. − In particular, the invention of base-pair-driven toehold-mediated strand displacement (BP-TMSD) , has served as a founding principle to generate functional DNA-based machines − including tweezers, , autonomous walkers, , molecular diagnostic agents, ,− and higher-order networksthat show neural mimicry, , control intra/intercell interactions, − and perform computational tasks. − In BP-TMSD, an invading fuel sequence uses Watson–Crick–Franklin-based toehold/toe interactions to achieve isothermal displacement of an output sequence from a stable duplex substrate (Figure A). This system couples an input to the release of a specific output and can be integrated into functional machines and layered reactions.…”

Section: Introductionmentioning

confidence: 99%

DNA Strand Displacement Driven by Host–Guest Interactions

et al. 2022

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Base-pair-driven toehold-mediated strand displacement (BP-TMSD) is a fundamental concept employed for constructing DNA machines and networks with a gamut of applications�from theranostics to computational devices. To broaden the toolbox of dynamic DNA chemistry, herein, we introduce a synthetic surrogate termed host−guest-driven toeholdmediated strand displacement (HG-TMSD) that utilizes bioorthogonal, cucurbit[7]uril (CB[7]) interactions with guest-linked input sequences. Since control of the strand-displacement process is salient, we demonstrate how HG-TMSD can be finely modulated via changes to the structure of the input sequence (including synthetic guest head-group and/or linker length). Further, for a given input sequence, competing small-molecule guests can serve as effective regulators (with fine and coarse control) of HG-TMSD. To show integration into functional devices, we have incorporated HG-TMSD into machines that control enzyme activity and layered reactions that detect specific microRNA.

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“…Stimuli-responsive, reversible cross-linker makes the hydrogel ‘smart’, which changes its properties at certain predefined environmental conditions or responses to small molecules or ionic cues 6 . There are many examples of stimuli-responsive DNA structures that follow Watson-crick base paring as well as non-Watson-crick base paring 7 . To make the hydrogels functional, different motifs and functional groups have been used, including aptamers 8 , i-motifs 9 , G-quadruplexs 10 , antibodies 11 , small peptides 12 etc.…”

Section: Introductionmentioning

confidence: 99%

pH - responsive, reversible A-motif based DNA hydrogels: synthesis and biosensing applications

Morya

Shukla

Ghoroi

et al. 2022

Preprint

Self Cite

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Functional DNA hydrogels using various motifs and functional groups require perfect sequence designing to avoid cross-bonding interference with self or other structural sequences. The present work reports an A-motif functional DNA hydrogel that does not require any sequence design. A-motif DNA is a non-canonical parallel DNA duplex structure comprises homopolymeric deoxyadenosines (poly-dA) strands that undergo conformational changes from single strands at neutral pH to a parallel duplex DNA helix at acidic pH. Despite many advantages over other DNA motifs like no sequence, design is required and no cross-bonding interference with other structural sequences, A-motif has not been explored much. We successfully synthesized DNA hydrogel utilizing A-motif as a reversible handle to polymerize DNA three-way junction (3WJ). The composed A-motif hydrogel was first characterized by EMSA, & DLS, which shows the formation of higher-order structures. Further, we utilized imaging techniques like atomic force microscopy (AFM) and scanning electron microscope (SEM) validating its hydrogel like highly branched morphology. pH-induced conformational transformation from monomers to gel is quick and reversible, and was analysed for multiple acid-base cycles. The sol-to-gel transitions and gelation properties is further examined using rheological studies. The use of A-motif hydrogel in the visual detection of pathogenic target nucleic acid sequence is demonstrated for the first time using the capillary assay. Moreover, the pH-induced hydrogel formation is observed in-situ as a layer over the mammalian cells. The proposed A-motif DNA scaffold has enormous potential in designing stimuli-responsive nanostructures that can be utilized for many biological applications.

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Stimuli Responsive, Programmable DNA Nanodevices for Biomedical Applications

Cited by 13 publications

References 131 publications

DNA‐Based Nanoarchitectures as Eminent Vehicles for Smart Drug Delivery Systems

DNA‐Based Nanoarchitectures as Eminent Vehicles for Smart Drug Delivery Systems

DNA Strand Displacement Driven by Host–Guest Interactions

pH - responsive, reversible A-motif based DNA hydrogels: synthesis and biosensing applications

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