Stimuli‐Responsive Nanostructured Surfaces for Biomedical Applications

Gomes, Bárbara Santos; Mendes, Paula M.

doi:10.1002/9783527807369.ch6

Cited by 1 publication

(2 citation statements)

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“…On-demand specific capture of antibodies on surfaces provides the opportunity for detection only when required, leading to the development of sophisticated multianalyte sensors. Such sensors could be applied, for instance, in the near real-time, long-term monitoring of biological processes in cell culture systems or in diagnostic devices for disease detection and surveillance on-demand. , …”

Section: Introductionmentioning

confidence: 99%

“…Such sensors could be applied, for instance, in the near real-time, long-term monitoring of biological processes in cell culture systems or in diagnostic devices for disease detection and surveillance ondemand. 10,11 Surfaces with temporal control attributes are the focus of many recent efforts, where materials exhibit the ability to modulate their properties in response to diverse external stimuli, such as electric, optical, thermal, and chemical. 12−14 Surfaces have been created that are able to control bacterial adhesion via modulation of nonspecific interactions.…”

Section: Introductionmentioning

confidence: 99%

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On-Demand Electrical Switching of Antibody–Antigen Binding on Surfaces

Gomes

Cantini

Tommasone

et al. 2018

ACS Appl. Bio Mater.

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The development of stimuli-responsive interfaces between synthetic materials and biological systems is providing the unprecedented ability to modulate biomolecular interactions for a diverse range of biotechnological and biomedical applications. Antibody−antigen binding interactions are at the heart of many biosensing platforms, but no attempts have been made yet to control antibody−antigen binding in an on-demand fashion. Herein, a molecular surface was designed and developed that utilizes an electric potential to drive a conformational change in surface bound peptide moiety, to give on-demand control over antigen−antibody interactions on sensor chips. The molecularly engineered surfaces allow for propagation of conformational changes from the molecular switching unit to a distal progesterone antigen, resulting in promotion (ON state) or inhibition (OFF state) of progesterone antibody binding. The approach presented here can be generally applicable to other antigen−antibody systems and meets the technological needs for in situ long-term assessment of biological processes and disease monitoring ondemand.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%