Stimuli-responsive materials: A smart way to study dynamic cell responses

Bril, Maaike; Fredrich, Sebastian; Kurniawan, Nicholas A.

doi:10.1016/j.smaim.2022.01.010

Cited by 39 publications

(27 citation statements)

References 167 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, an interplay between the two cell types is necessary for the overall strain-induced response that leads to anisotropic cell organization as is expected in healthy, contractile myocardium. Therefore, our results propose an intriguing emergence of guidance cues for CMs that are mediated by the mechanoresponsiveness of cFBs; whereas cell orientation can be dynamically induced by topographies and structural guidance cues in in vitro culture using light-responsive synthetic materials, 74–76 we present a similar emergence arising from cues that are inherent in the in vivo CM microenvironment—mechanical strain and cFBs. Further investigation should address if the removal of cyclic strain can cease the presentation of the cFB-mediated guidance cues, which would suggest dynamic control over the presentation of these guidance cues.…”

Section: Discussionmentioning

confidence: 70%

Human pluripotent stem cell-derived cardiomyocytes align under cyclic strain when guided by cardiac fibroblasts

et al. 2022

Self Cite

View full text Add to dashboard Cite

The myocardium is a mechanically active tissue typified by anisotropy of the resident cells [cardiomyocytes (CMs) and cardiac fibroblasts (cFBs)] and the extracellular matrix (ECM). Upon ischemic injury, the anisotropic tissue is replaced by disorganized scar tissue, resulting in loss of coordinated contraction. Efforts to re-establish tissue anisotropy in the injured myocardium are hampered by a lack of understanding of how CM and/or cFB structural organization is affected by the two major physical cues inherent in the myocardium: ECM organization and cyclic mechanical strain. Herein, we investigate the singular and combined effect of ECM (dis)organization and cyclic strain in a two-dimensional human in vitro co-culture model of the myocardial microenvironment. We show that (an)isotropic ECM protein patterning can guide the orientation of CMs and cFBs, both in mono- and co-culture. Subsequent application of uniaxial cyclic strain—mimicking the local anisotropic deformation of beating myocardium—causes no effect when applied parallel to the anisotropic ECM. However, when cultured on isotropic substrates, cFBs, but not CMs, orient away from the direction of cyclic uniaxial strain (strain avoidance). In contrast, CMs show strain avoidance via active remodeling of their sarcomeres only when co-cultured with at least 30% cFBs. Paracrine signaling or N-cadherin-mediated communication between CMs and cFBs was no contributing factor. Our findings suggest that the mechanoresponsive cFBs provide structural guidance for CM orientation and elongation. Our study, therefore, highlights a synergistic mechanobiological interplay between CMs and cFBs in shaping tissue organization, which is of relevance for regenerating functionally organized myocardium.

show abstract

Section: Discussionmentioning

confidence: 70%

Human pluripotent stem cell-derived cardiomyocytes align under cyclic strain when guided by cardiac fibroblasts

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Importantly, an interplay between the two cell types is necessary for the overall strain-induced response that leads to anisotropic cell organization as is expected in healthy, contractile myocardium. Therefore, our results propose an intriguing emergence of guidance cues for CMs that are mediated by the mechanoresponsiveness of cFBs, Whereas cell orientation can be dynamically induced by topographies and structural guidance cues in in vitro culture using light-responsive synthetic materials [51 – 53],we present a similar emergence arising from cues that are inherent in the in vivo CM microenvironment – mechanical strain and cFBs. Further investigation should address if removal of cyclic strain can cease the presentation of the cFB-mediated guidance cues, which would suggest dynamic control over the presentation of these guidance cues.…”

Section: Discussionmentioning

confidence: 85%

Human pluripotent stem cell-derived cardiomyocytes align under cyclic strain when guided by cardiac fibroblasts

Mostert

Groenen

Klouda

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

The human myocardium is a mechanically active tissue typified by the anisotropic organization of cells and extracellular matrix (ECM). Upon injury, the composition of the myocardium changes, resulting in disruption of tissue organization and loss of coordinated contraction. Understanding how anisotropic organization in the adult myocardium is shaped and disrupted by environmental cues is thus critical, not only for unravelling the processes taking place during disease progression, but also for developing regenerative strategies to recover tissue function. Here, we decoupled in vitro the two major physical cues that are inherent in the myocardium: structural ECM and mechanical strain. We show that patterned ECM proteins control the orientation of the two main cell types in the myocardium: human cardiac fibroblasts (cFBs) and cardiomyocytes (hiPSC-CMs), despite their different mechanosensing machinery. Uniaxial cyclic strain, mimicking the local anisotropic deformation of the myocardium, did not affect hiPSC-CMs orientation. It did however induce a reorientation of cFBs, perpendicular to the strain direction, albeit this strain-avoidance response was overruled in the presence of anisotropic structural cues. These findings reveal that the mechanoresponsiveness of cFBs may be a critical handle in controlling myocardial tissue structure and function. To test this, we co-cultured hiPSC-CMs and cFBs in varying cell ratios to reconstruct normal and pathological myocardium. Contrary to the hiPSC-CM monoculture, the co-cultures adopted an anisotropic organization under uniaxial cyclic strain, regardless of the cell ratio. Together, these results identify the cFBs as a therapeutic target to mechanically restore structural organization of the tissue in cardiac regenerative therapies.

show abstract

“…Copyright 2021 Wiley Online Library, (c) role of dynamimotions in bronchial stretching, fibrosis, wound healing, and scar formation, heart beating and embryonic developments. Reprinted with permission from ref . Copyright 2022 Elsevier.…”

Section: Importance Of Surface Topography In Scaffold Designmentioning

confidence: 99%

“…In this line, a variety of polymers possessing the exclusive feature of stimuli-responsiveness has been utilized with the aim of bioactive environment remodeling. By providing these susceptible conditions, effective cell–substrate interactions and modulation of cells anisotropy are occurring. , Past studies on topological properties of biological cell membranes have revealed the nonuniformity of surface tension leading to anisotropic cellular motions and the creation of superficial tension gradient causing to dynamical deformation of biological cells. ,, The simplest example of this biophysical nature of cells is filamentous structures of single-cell cytoskeletons indicating the nematic liquid crystalline properties. − …”

Section: Introductionmentioning

confidence: 99%

“…1,2 Past studies on topological properties of biological cell membranes have revealed the nonuniformity of surface tension leading to anisotropic cellular motions and the creation of superficial tension gradient causing to dynamical deformation of biological cells. 1,3,4 The simplest example of this biophysical nature of cells is filamentous structures of single-cell cytoskeletons indicating the nematic liquid crystalline properties. 5−7 Liquid crystalline elastomers (LCEs) with a solid-elastic surface and programmable liquid crystalline phase are the new generation of smart materials for developing tissue-engineered scaffolds which are able to adjust almost every chemical, mechanical and morphological parameters.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Performance of Liquid Crystalline Elastomers on Biological Cell Response: A Review

Fallah-Darrehchi

Zahedi

Harirchi

et al. 2023

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

Dynamic interplay between extracellular matrix with anisotropic structure and biological cells has directed the scaffold path of development toward the utilization of actuation-responsive biomaterials. Among stimuliresponsive polymers, liquid crystalline elastomers (LCEs), owing to attachment of highly anisotropic mesogenic units to the amorphous elastomeric chains, are able to reveal reversible orientational order. Dynamic order− disorder phase transition and stimuli-responsiveness are the main factors to consider them as rationally smart mimickers of biological cell topology. This Review explains briefly the structures and synthesis methods of LCEs as well as their materiobiology with an emphasis on biochemical and biomechanical features of cells from molecular view. In this regard, some indispensable factors such as quasi-liquid crystalline behavior of cells, degree of orientational order in different cells and liquid crystalline induced topographical signals as growth enhancing agent for cells are investigated. Moreover, the interactions of cellliquid crystalline substrate and the performance of LCE scaffolds on various cell lines response are given as an overview.

show abstract

Stimuli-responsive materials: A smart way to study dynamic cell responses

Cited by 39 publications

References 167 publications

Human pluripotent stem cell-derived cardiomyocytes align under cyclic strain when guided by cardiac fibroblasts

Human pluripotent stem cell-derived cardiomyocytes align under cyclic strain when guided by cardiac fibroblasts

Human pluripotent stem cell-derived cardiomyocytes align under cyclic strain when guided by cardiac fibroblasts

Performance of Liquid Crystalline Elastomers on Biological Cell Response: A Review

Contact Info

Product

Resources

About