Stimuli‐Responsive Cucurbit[7]uril‐Mediated BSA Nanoassembly for Uptake and Release of Doxorubicin

Abstract

We report the construction of a non-toxic nanoassembly of bovine serum albumin (BSA) protein and the cucurbit[7]uril macrocycle as well as its stimuli-responsive breakage with adamantylamine or pH, which restores the protein structure and recognition properties. The assembly showed efficient loading and controlled release of a standard drug, doxorubicin (DOX), and the same was validated in live cells. The cell viability studies documented that the DOX-loaded assembly mask the cytotoxicity of DOX and the toxicity can be revived at the target on demand, triggering its therapeutic activation. This is found to be more effective in the cancer cells. In addition, such host-assisted protein assemblies are also highly promising for stabilizing/protecting the native protein structure, a viable approach to prevent/inhibit protein misfolding and aggregation.

Photo‐ and pH‐ Dual‐Responsive β‐Cyclodextrin‐Based Supramolecular Prodrug Complex Self‐Assemblies for Programmed Drug Delivery

“…[1][2][3][4][5][6] However,l eakage of the toxic drugs encapsulatedi nt he self-assemblies may occur along with circulation, which can lead to damage to normal tissue. [1][2][3][4][5][6] However,l eakage of the toxic drugs encapsulatedi nt he self-assemblies may occur along with circulation, which can lead to damage to normal tissue.…”

Stimuli‐responsive Supra‐biomolecular Nanoassemblies of Cucurbit[7]uril with Bovine Serum Albumin: Drug Delivery and Sensor Applications

“…[1][2][3][4] In this context, supramolecular assemblies of proteins with macrocyclic hosts are extremely interesting, both from the view point of its fundamental/mechanistic aspects and also on the details of its applied potentials. [1][2][3][4] In this context, supramolecular assemblies of proteins with macrocyclic hosts are extremely interesting, both from the view point of its fundamental/mechanistic aspects and also on the details of its applied potentials.…”

“…[8] On the other hand, among several biomolecules of vital importance, proteins are considered to be the nature's primary macromolecules that carry out complex chemical transformations and various metabolic tasks. [3] Therefore, the uptake and release mechanism of proteins has always been an active research topic pertinent to the development of drug delivery systems. [20][21][22] In this perspective, macromolecules or cavitands that recognize the side chains of the aminoacids residues can act as generic surface binders, which can affect/alter the amphiphilic characteristics and the overall protein structure, stability and activity.…”

“…[25] Lei et al have shown that the higher homologue of CB7 i.e cucurbit [8]uril (CB8) can improve the binding affinity of 5,10,15,20-tetrakis(1-methyl-4-pyridinio) porphyrin-tetra(ptoluenesulfonate) (TMPyP) greatly towards BSA. [3] On the other hand, the cooperative interaction of BG with the CB7-BSA assembly leads to a significant cumulative fluorescence enhancement, where CB7 acts as supramolecular enhancer. In an earlier study, our group has shown that CB8 forms 1 : 2 complexes with the protonated form of neutral red dye.…”

pH-responsive molecular assemblies of pyridylbutadiene derivative with cucurbit[7]uril

“…7,12 Among all the known CBs, cucurbit [7]uril (CB7) and cucurbit [8]uril (CB8) were extensively used for these investigations because of their water solubility and suitability of their cavity size for encapsulating different guest molecules. 6,[13][14][15][16][17] Cucurbiturils have been utilized as a template for stereoselective photodimerization, 18 supramolecular catalysis, 19 inhibition and disintegration of amyloid brils for therapeutics, 20 radionuclide separation, 21 construction of supramolecular assemblies, 22,23 and to modulate photophysical properties 10,24 for several cationic pyridinium or viologen derivatives. 6,10,18,[24][25][26][27][28][29] Inspired from these earlier reports, we describe optical absorption and emission properties of a cationic pyridinium derivative [4-((1E,3E)-4-(4-(dimethylamino)phenyl)buta-1,3-dien-1-yl)-1-methylpyridin-1-ium (DABP)], in water in the absence and presence of CB7.…”