Stimuli responsive chiral liquid crystal phases of phenylboronic acid functionalized rodlike viruses and their interaction with biologically important diols

Cao, Jun; Liu, Shuaiyu; Xiong, Jie; Chen, Yingjun; Zhang, Zhenkun

doi:10.1039/c4cc04639k

Cited by 16 publications

(19 citation statements)

References 41 publications

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“…Dark lines correspond to regions where the rods are perpendicular to the plane of the paper and bright lines correspond to regions where rods are in the plane of paper. Panels A and B reprinted with permission from ref . Copyright 2014 Royal Society of Chemistry.…”

Section: Filamentous Phage Hydrogels and Liquid Crystalsmentioning

confidence: 99%

“…Iridescence is a result of interference between reflections from two or more single layers of aligned phage. When this interference is constructive, the film appears light, and when it is destructive, the surface appears dark (Figure A–C) …”

Section: Filamentous Phage Hydrogels and Liquid Crystalsmentioning

confidence: 99%

“…Furthermore, surface modifications of the coat proteins on filamentous phages can affect the process of self-assembly. For example, phenylboronic acids have been used to modify the surfaces of M13 bacteriophages . This modification allows M13 to form pure nematic or chiral nematic liquid crystalline phases in a pH-dependent manner .…”

Section: Filamentous Phage Hydrogels and Liquid Crystalsmentioning

confidence: 99%

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Filamentous Phages as Building Blocks for Bioactive Hydrogels

Jackson

Peivandi

Fogal

et al. 2021

ACS Appl. Bio Mater.

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Filamentous bacteriophages (bacterial viruses) are semiflexible proteinous nanofilaments with high aspect ratios for which the surface chemistry can be controlled with atomic precision via genetic engineering. That, in addition to their ability to self-propagate and replicate a nearly monodisperse batch of biologically and chemically identical nanofilaments, makes these bionanofilaments superior to most synthetic nanoparticles and thus a powerful tool in the bioengineers’ toolbox. Furthermore, filamentous phages form liquid crystalline structures at high concentrations; these ordered assemblies create hierarchically ordered macro-, micro-, and nanostructures that, once cross-linked, can form hierarchically ordered hydrogels, hydrated soft material with a variety of physical and chemical properties suitable for biomedical applications (e.g., wound dressings and tissue engineering scaffolds) as well as biosensing, diagnostic assays. We provide a critical review of these hydrogels of filamentous phage, and their physical, mechanical, chemical, and biological properties and current applications, as well as an overview of limitations and challenges and outlook for future applications. In addition, we present a list of design parameters for filamentous phage hydrogels to serve as a guide for the (bio)engineer and (bio)chemist interested in utilizing these powerful bionanofilaments for designing smart, bioactive materials and devices.

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Section: Filamentous Phage Hydrogels and Liquid Crystalsmentioning

confidence: 99%

Section: Filamentous Phage Hydrogels and Liquid Crystalsmentioning

confidence: 99%

Section: Filamentous Phage Hydrogels and Liquid Crystalsmentioning

confidence: 99%

See 1 more Smart Citation

Filamentous Phages as Building Blocks for Bioactive Hydrogels

Jackson

Peivandi

Fogal

et al. 2021

ACS Appl. Bio Mater.

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“…In contrast, chemical modification is a much versatile way to functionalize the M13 virus, thanks to the rich surface chemical groups of the solvent-exposed amino acid residuals of the p8 coat proteins. 20 , 28 − 31 Therefore, by exploring the well-known affinity of the thiol group for gold, M13 viruses modified with thiol groups (M13-SH) have been used as the efficient templates for gold reduction or assembling of preformed gold nanoparticles. 19 , 20 , 28 , 30 , 32 Convenience, versatility of chemical modifications and diverse metal affinity of the thiol groups are the advantages of M13-SH for gold extraction.…”

Section: Introductionmentioning

confidence: 99%

Bioreduction of Gold Ions under Greener Conditions by the Thiol-Modified M13 Bacteriophage and with Hydroxylamine as the Autocatalytic Reducing Agent

et al. 2022

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Bioreduction of gold ions by the thiol-modified M13 bacteriophage (M13-SH) has been exploited as the potential alternative to conventional methods based on toxic chemicals, due to the gold affinity of the thiol groups, inherent gold reduction, and high specific surface area of the filamentous virus. Such efforts have been hindered by harsh conditions involving strong reducing agents and extreme pH that are harmful to the virus. Herein, a virus-friendly and greener method of bioreduction of AuCl 4 – at neutral pH based on M13-SH is demonstrated. M13-SH was prepared by coupling the virus with N -succinimidyl S -acetylthioacetate, followed by deacylation in the presence of hydroxylamine·HCl to expose the thiol groups. The key finding is that without time-consuming purification, the mixture after deacylation consisting of M13-SH, residual hydroxylamine, and so forth can directly turn ionic gold species into gold, leading to macroscopic precipitated products with interconnected linear structures consisting of fused gold nanoparticles. Besides working as the virus-friendly reducing agent with a unique autocatalytic style, hydroxylamine diminishes disulfide bonding-induced intervirus bundling of M13-SH so as to maintain its efficient biosorption of ionic gold precursors. This work demonstrates a general and green strategy of bioreduction of gold via combination of the gold-affinity proteins or organisms and the unique autocatalytic reduction of hydroxylamine.

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“…7 Stimulated by such behavior, we conjecture that, for a given kind of viruses such as M13, their chiral ordering in the CLC phase might be tunable if there is some means to control the encoded unique assembling information of the viruses. 29 How will such parameters such as concentration (in the nematic phase range), ionic strength, exert influence on such behavior? Especially, the underlying mechanism of such chiral to non-chiral transformation is unclear.…”

Section: Introductionmentioning

confidence: 99%

Thermoresponsive Chiral to Nonchiral Ordering Transformation in the Nematic Liquid-Crystal Phase of Rodlike Viruses: Turning the Survival Strategy of a Virus into Valuable Material Properties

et al. 2015

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The current work investigates the thermoresponsive in situ chiral to nonchiral ordering transformation of a rodlike virus in the naturally assembled state-the chiral nematic liquid crystal (CLC) phase. We take this as an elegant example of reconfigurable self-assembly, through which it is possible to realize in situ transformation from one assembled state to another without disrupting the preformed assembly in general or going through a secondary assembling procedure of the disassembled building blocks. The detailed investigation presented here reveals many unique characteristics of the thermoresponsive 3D chiral ordering of rodlike viruses induced by heat stress. The chiral to nonchiral ordering transformation is highly reversible in the temperature range of up to 60 °C and can be repeated many times. There exists a critical temperature around 40 °C which is independent of the ionic strength and virus concentration. Such reconfigurable ordering in the CLC phase stems from the intrinsic structure change of constituent coat proteins without disrupting the structural integrity of the virus, as revealed by three analytical techniques targeting levels ranging from the molecular, secondary conformation of the constituent proteins to the whole single virus, respectively. Such structural flexibility, also termed polymorphism, is relative to the survival strategies of a biological organism such as the virus and can be transformed into very precious material properties. The potential of the virus-based CLC phase as the chiral matrix to regulate chiro-optical properties of gold nanorods is also presented.

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Stimuli responsive chiral liquid crystal phases of phenylboronic acid functionalized rodlike viruses and their interaction with biologically important diols

Cited by 16 publications

References 41 publications

Filamentous Phages as Building Blocks for Bioactive Hydrogels

Filamentous Phages as Building Blocks for Bioactive Hydrogels

Bioreduction of Gold Ions under Greener Conditions by the Thiol-Modified M13 Bacteriophage and with Hydroxylamine as the Autocatalytic Reducing Agent

Thermoresponsive Chiral to Nonchiral Ordering Transformation in the Nematic Liquid-Crystal Phase of Rodlike Viruses: Turning the Survival Strategy of a Virus into Valuable Material Properties

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