Stimulatory function of peroxiredoxin 1 in activating adaptive humoral immunity in a zebrafish model

Liu, Guang-ping; Xiang, Lei; Shao, Tong; Shao, Jian-zhong

doi:10.1016/j.dci.2018.03.004

Cited by 11 publications

(7 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the mechanism of inhibition in previous studies remains unclear and requires further investigation. Further studies found that PRDX1 had the strongest positive correlation with type 2T helper cells, consistent with previous studies 73,74 , and GPX4 had the strongest negative correlation with neutrophils, consistent with previous studies 75,76 . miRNAs play an important role in IS development 77 .…”

Section: Discussionsupporting

confidence: 91%

Analysis and identification of oxidative stress-ferroptosis related biomarkers in ischemic stroke

Zhang,

Liang,

Xiong

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Studies have shown that a series of molecular events caused by oxidative stress is associated with ferroptosis and oxidation after ischemic stroke (IS). Differential analysis was performed to identify differentially expressed mRNA (DEmRNAs) between IS and control groups. Critical module genes were identified using weighted gene co-expression network analysis (WGCNA). DEmRNAs, critical module genes, oxidative stress-related genes (ORGs), and ferroptosis-related genes (FRGs) were crossed to screen for intersection mRNAs. Candidate mRNAs were screened based on the protein–protein interaction (PPI) network and the MCODE plug-in. Biomarkers were identified based on two types of machine learning algorithms, and the intersection was obtained. Functional items and related pathways of the biomarkers were identified using gene set enrichment analysis (GSEA). Finally, single-sample GSEA (ssGSEA) and Wilcoxon tests were used to identify differential immune cells. An miRNA-mRNA-TF network was created. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the expression levels of biomarkers in the IS and control groups. There were 8287 DE mRNAs between the IS and control groups. The genes in the turquoise module were selected as critical module genes for IS. Thirty intersecting mRNAs were screened for overlaps. Seventeen candidate mRNAs were also identified. Four biomarkers (CDKN1A, GPX4, PRDX1, and PRDX6) were identified using two types of machine-learning algorithms. GSEA results indicated that the biomarkers were associated with steroid biosynthesis. Nine types of immune cells (activated B cells and neutrophils) were markedly different between the IS and control groups. We identified 3747 miRNA-mRNA-TF regulatory pairs in the miRNA-mRNA-TF regulatory network, including hsa-miR-4469-CDKN1A-BACH2 and hsa-miR-188-3p-GPX4-ATF2. CDKN1A, PRDX1, and PRDX6 were upregulated in IS samples compared with control samples. This study suggests that four biomarkers (CDKN1A, GPX4, PRDX1, and PRDX6) are significantly associated with IS. This study provides a new reference for the diagnosis and treatment of IS.

show abstract

Section: Discussionsupporting

confidence: 91%

Analysis and identification of oxidative stress-ferroptosis related biomarkers in ischemic stroke

Zhang,

Liang,

Xiong

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Prdx1 is a highly abundant cytosolic thioredoxin-dependent peroxidase and appears to be more efficient at removing H 2 O 2 and organic hydroperoxides. Prdx1 is important for vascular development in zebrafish ( Huang et al, 2017 ), and has a stimulatory role in the initiation of adaptive humoral immunity ( Liu et al, 2018 ). Different isoforms of human peroxiredoxin-5 exist which localise to the cytosol, mitochondria or peroxisomes.…”

Section: Resultsmentioning

confidence: 99%

Insights Into the Peroxisomal Protein Inventory of Zebrafish

et al. 2022

View full text Add to dashboard Cite

Peroxisomes are ubiquitous, oxidative subcellular organelles with important functions in cellular lipid metabolism and redox homeostasis. Loss of peroxisomal functions causes severe disorders with developmental and neurological abnormalities. Zebrafish are emerging as an attractive vertebrate model to study peroxisomal disorders as well as cellular lipid metabolism. Here, we combined bioinformatics analyses with molecular cell biology and reveal the first comprehensive inventory of Danio rerio peroxisomal proteins, which we systematically compared with those of human peroxisomes. Through bioinformatics analysis of all PTS1-carrying proteins, we demonstrate that D. rerio lacks two well-known mammalian peroxisomal proteins (BAAT and ZADH2/PTGR3), but possesses a putative peroxisomal malate synthase (Mlsl) and verified differences in the presence of purine degrading enzymes. Furthermore, we revealed novel candidate peroxisomal proteins in D. rerio, whose function and localisation is discussed. Our findings confirm the suitability of zebrafish as a vertebrate model for peroxisome research and open possibilities for the study of novel peroxisomal candidate proteins in zebrafish and humans.

show abstract

“…Peroxiredoxins have been identified in various organisms ranging from prokaryotes to eukaryotes, playing an important role in protecting organisms against oxidative stress [ 37 ]. In fish, reports on peroxiredoxins mainly focused on Prx1 and Prx2 [ 26 , 38 ], while information about Prx3 is limited. Herein, the Prx3 gene from grass carp was cloned, and its role in antioxidant activity and GCRV replication was analyzed.…”

Section: Discussionmentioning

confidence: 99%

Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication

Chu

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Peroxiredoxins are a family of antioxidant proteins that protect cells from oxidative damage caused by reactive oxygen species (ROS). Herein, the peroxiredoxin 3 gene from grass carp (Ctenopharyngodon idellus), named CiPrx3, was cloned and analyzed. The full-length cDNA of CiPrx3 is 1068 bp long, with a 753 bp open reading frame (ORF) that contains a thioredoxin-2 domain, two peroxiredoxin signature motifs, and two highly conserved cysteine residues. CiPrx3 was ubiquitously expressed in all the tested tissues, while its expression level was altered significantly after exposure to grass carp reovirus (GCRV) and pathogen-associated molecular patterns (PAMPs). CiPrx3 was localized in the mitochondria of transfected cells and concentrated in the nucleus after poly (I:C) treatment. Transformation of CiPrx3 into Escherichia coli enhanced host resistance to H2O2 and heavy metals. Purified recombinant CiPrx3 proteins could protect DNA against oxidative damage. Overexpression of CiPrx3 in fish cells reduced intracellular ROS, increased cell viability, and decreased cell apoptosis caused by H2O2 stimulation and GCRV infection. Further study indicated that CiPrx3 induced autophagy to inhibit GCRV replication in fish cells. Collectively, these results imply that grass carp Prx3 elevates host antioxidant activity and induces autophagy to inhibit GCRV replication.

show abstract

Stimulatory function of peroxiredoxin 1 in activating adaptive humoral immunity in a zebrafish model

Cited by 11 publications

References 46 publications

Analysis and identification of oxidative stress-ferroptosis related biomarkers in ischemic stroke

Analysis and identification of oxidative stress-ferroptosis related biomarkers in ischemic stroke

Insights Into the Peroxisomal Protein Inventory of Zebrafish

Grass Carp Prx 3 Elevates Host Antioxidant Activity and Induces Autophagy to Inhibit Grass Carp Reovirus (GCRV) Replication

Contact Info

Product

Resources

About