Stimulatory effects of fish oil and vitamin E on ovarian function of laying hen

Abstract

In recent years, there has been an increase in the consumption of omega-6 fatty acids (FAs) over omega-3. Moreover, the antioxidant nature of oil supplemented diet in human and animal studies is not clear. In this work, the n-3 effects on hen ovarian cells, ovarian dynamics, serum FA profiles and expression level of apoptosis genes in the presence or absence of vitamin E were studied. Sixty-eight laying hens were randomly divided into four groups and the groups were fed the following diets: basal diet þ1.5% sunflower oil (control; C); basal diet þ1.5% sunflower oil þ1.1 U alpha-tocopherol/hen/day (E); basal diet þ1.5% fish oil þ1.1 U alphatocopherol/hen/day (n-3 þ E); and basal diet þ1.5% fish oil (n-3). In E, n-3 þ E and n-3, small yellow follicle numbers were higher than control (p < .05). Large yellow follicle numbers as well as total numbers of follicles of n-3 þ E and n-3 were higher (p < .05) than control and E. The rate of ovulation was significantly (p < .05) higher in n-3 (96%) compared to the control (89%). The proportion of intact primordial follicles in n-3 and n-3 þ E was significantly more than control and E (p < .01). The expression of Bak (Bcl2 antagonist/killer) was significantly (p < .05) higher in control than the other groups. There was significant reduction in expression of Cas9 in n-3 and E compared to the control and n-3 þ E (p < .05). While omega-3 and vitamin E can protect ovarian cells from apoptotic death, omega-3 source could have promoting roles on follicular development and ovulation rate, being independent of dietary vitamin E. HIGHLIGHTSDespite the perceived importance of supplementing antioxidant with fatty acids, our results indicated that alone low level of n-3 has a great influence on ovary performance. ARTICLE HISTORY

Inclusion of ovine enriched serum with vitamin E and polyunsaturated fatty acids in the freezing medium: a new strategy to improve human frozen‐thawed sperm parameters

“…To minimise the loss of membrane PUFAs during freeze–thaw procedure, dietary supplementation of PUFAs or direct addition of PUFAs enriched sources in cryopreservation medium has been reported in human and animals trials (Abavisani, Arshami, Naserian, Kandelousi, & Azizzadeh, ; Abdi‐Benemar et al, ; Esmaeili, Shahverdi, Alizadeh, Alipour, & Chehrazi, ; Habibi et al, ; Kaeoket, Sang‐urai, Thamniyom, Chanapiwat, & Techakumphu, ; Safarinejad, ; Towhidi, Zeinoaldini, Ardebili, Dadashpour Davachi, & Nasiri, ). Dietary supplementation with fish oil increased the level of the n–3 fatty acids in the serum (Nateghi, Alizadeh, Jafari Ahangari, Fathi, & Akhlaghi, ; Safarinejad, ) and sperm plasma membrane (Alizadeh, Esmaeili, Shahverdi, & Rashidi, ; Esmaeili et al, ; Safarinejad, ). Moreover, improvement in frozen‐thawed sperm quality by dietary supplementation with PUFAs has been well established in previous studies (Esmaeili et al, ; Habibi et al, ).…”

Numbers of Follicles, Pregnancy, Expression of Reactive Oxygen Species (ROS), and Ovarian Genes in Sheep Treated with Vitamin E, L-Carnitine, and Fish Oil

“…The decreased ROS levels in female follicular fluid increased the number of follicles and increased fertility (Attaran et al, 2000;Elizur et al, 2014). The presence of vitamin E along with omega 3 in the diet can play a role in boosting follicle growth (Nateghi et al, 2019). In the present study, it was shown that the use of vitamin E and L-carnitine increased the expression of TGF-β in the ovary, which led to the improved follicle function and pregnancy; this could be due to the improved inflammation in the tissue as well as the better function of the ovary.…”