Stimulatory effect of low ATP pools on transport of purine nucleosides in cells of Escherichia coli.

Munch‐Petersen, Agnete; Pihl, Niels Jorgen

doi:10.1073/pnas.77.5.2519

Cited by 7 publications

(4 citation statements)

References 17 publications

(20 reference statements)

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“…The results reported have not yet presented unequivocal proof concerning the mechanism(s) of nucleoside or base transport. Some authors favor a group translocation mechanism (5,18), whereas others implicate an active transport mechanism (3,4,8,9,11). Munch-Petersen and Pihl (8) propose a proton motive force as the main energizer of nucleoside transport.…”

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confidence: 99%

“…Some authors favor a group translocation mechanism (5,18), whereas others implicate an active transport mechanism (3,4,8,9,11). Munch-Petersen and Pihl (8) propose a proton motive force as the main energizer of nucleoside transport. Burton (3) presents evidence for porter systems for adenine, hypoxanthine, and uracil dependent on a proton motive force and facilitated by intracellular metabolism of the free bases.…”

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confidence: 99%

“…Burton (3) presents evidence for porter systems for adenine, hypoxanthine, and uracil dependent on a proton motive force and facilitated by intracellular metabolism of the free bases. Most authors propose the existence of more than one transport system (4,7,8,11). The interpretation of such transport data is often complicated by several interconversions of substrates and resulting products by enzymes located in the cytoplasm, at the inner or outer surface of the plasma membrane, or in the periplasmic space (2,3,5,18).…”

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Interconversion and uptake of nucleotides, nucleosides, and purine bases by the marine bacterium MB22

Foret¹,

Ahlers²

1982

J Bacteriol

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Whole cells and isolated membranes of the marine bacterium MB22 converted nucleotides present in the external medium rapidly into nucleosides and then into bases. Nucleosides and purine bases formed were taken up by distinct transport systems. We found a high-affinity common transport system for adenine, guanine, and hypoxanthine, with a Km of 40 nM. This system was inhibited noncompetitively by purine nucleosides. In addition, two transport systems for nucleosides were present: one for guanosine with a Km of 0.8 ,uM and another one for inosine and adenosine with a Km of 1.4 ,uM. The nucleoside transport systems exhibited both mixed and noncompetitive inhibition by different nucleosides other than those translocated; purine and pyrimidine bases had no effect. The transport of nucleosides and purine bases was inhibited by dinitrophenol or azide, thus suggesting that transport is energy dependent. Inside the cell all of the substrates were converted mainly into guanosine, xanthine, and uric acid, but also anabolic products, such as nucleotides and nucleic acids, could be found. MATERIALS AND METHODS Organism. The marine bacterium MB22 was a kind gift from A. J. De Siervo, University of Maine at Orono. Some of the microbiological characteristics of MB22 were published recently (17). Chemicals. 8-14C-labeled adenine, guanine, hypoxanthine, adenosine, guanosine, AMP, and GMP were obtained from the "Commissariat a l'energie atomique" (Gif-sur-Yvette, France), and 'IC-labeled ino-471

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Interconversion and uptake of nucleotides, nucleosides, and purine bases by the marine bacterium MB22

Foret¹,

Ahlers²

1982

J Bacteriol

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“…Because the Km of the Ino phosphorylase is 0.2 mM (16), more than 20-fold higher than the Km of Ino uptake (Table 3), it is likely that the inhibition of Ino uptake by Guo resulted from the competition for a common Ino-Guo transport system. Transport systems for nucleosides have been demonstrated in E. coli (23,24). Extracellular Hyp inhibited neither Guo transport nor the Guo phosphorylase, because it did not inhibit growth on or uptake of Guo.…”

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confidence: 99%

Specificity and control of uptake of purines and other compounds in Bacillus subtilis

Beaman¹,

Hitchins²,

Ochi³

et al. 1983

J Bacteriol

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Certain nucleotides control adaptation to changing nutrition or differentiation (sporulation) resulting from a general nutritional deficiency. To maintain the adaptation or differentiation process, once it has started, it may have been important for cells to evolve several independent and metabolically controllable systems enabling the uptake and metabolism of various nucleic acid bases or nucleosides. We have analyzed the cellular reactions with these compounds by measuring both their effect on growth and their uptake in appropriately chosen auxotrophic and uptake mutants. We have found one uptake system for guanine and hypoxanthine, another one for guanosine and inosine, and three other systems for adenine, adenosine, and uracil. The uptake systems of guaninehypoxanthine and guanosine-inosine are inhibited by the stringent response to amino acid deprivation (increase of guanosine 5'-diphosphate-3'-diphosphate), but they do not depend on the concentration of GTP, which decreases during sporulation. In contrast, the uptake of Ura depends on the presence of GTP, regardless of whether a GTP decrease was produced by the stringent response or otherwise. This was the only uptake system whose decrease was always correlated with the onset of sporulation. The uptake of other compounds, e.g., a

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Cloning and Expression of Genes Encoding the nupG Nucleoside Transport System in Escherichia Coli

Munch‐Petersen¹,

Jensen²

1984

The Cell Membrane

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Stimulatory effect of low ATP pools on transport of purine nucleosides in cells of Escherichia coli.

Cited by 7 publications

References 17 publications

Interconversion and uptake of nucleotides, nucleosides, and purine bases by the marine bacterium MB22

Interconversion and uptake of nucleotides, nucleosides, and purine bases by the marine bacterium MB22

Specificity and control of uptake of purines and other compounds in Bacillus subtilis

Cloning and Expression of Genes Encoding the nupG Nucleoside Transport System in Escherichia Coli

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