Stimulatory and inhibitory effects of genistein on human uterine leiomyoma cell proliferation are influenced by the concentration

Moore, Alicia B.; Castro, Lysandra; Yu, Linda Chia‐Hui; Zheng, Xiaolin; Di, Xudong; Sifre, Maria I.; Kissling, Grace E.; Newbold, Retha R.; Bortner, Carl D.; Dixon, Darlene

doi:10.1093/humrep/dem185

Cited by 53 publications

(58 citation statements)

References 48 publications

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“…The growth inhibition function of the two isoflavones on cancer cells vary with concentration, cell type and exposure time. For example, pure genistein and daidzein might exhibit biphasic effect on cancer cells, that is, the compounds showed inhibitory effect at high concentrations but displayed activating function at low concentrations [15][16][17]; or lower concentrations had more obvious apoptosis induction than higher concentrations [18]. It has also been reported that the two compounds alone inhibited certain malignant phenotype of melanoma via different mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Soy-derived Isoflavones Inhibit HeLa Cell Growth by Inducing Apoptosis

Xiao

Huang

Geng

et al. 2011

Plant Foods Hum Nutr

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Isoflavones are among the major bioactive compounds found in a wide variety of plant-derived foods, especially in soybeans and soy-based foods. In this study, the effect of a soy-derived isoflavone mixture (designated as SI-I, containing 71% daidzein, 14.3% genistein and 14.7% glycitein) on HeLa cells and its mechanism were investigated. SI-I in concentration range 5-80 μg/ml significantly reduced the survival rate of HeLa cells by MTT assay, whereas showed no side effect on that of L929 cells. After HeLa cells were exposed to 10, 20 and 40 μg/ml SI-I for 4 days, typical apoptotic morphological changes, including nuclear fragmentation, cytoplasm shrinkage and decrease of cell volume, were observed by fluorescence microscope and CLSM, respectively. FCM analysis revealed that the percentages of early apoptotic cells with lost Δψm increased by 2.27, 2.74 and 4.05 folds respectively, compared with control. The results showed that SI-I inhibited HeLa cell growth through inducing apoptosis via the mitochondrial pathway and comparisons with reported data indicated that synergistic effect existed between the isoflavone species contained in SI-I. It is proposed that natural soy-derived isoflavones are potential candidates as chemotherapeutic agents against human cervical cancer.

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Section: Introductionmentioning

confidence: 99%

Soy-derived Isoflavones Inhibit HeLa Cell Growth by Inducing Apoptosis

Xiao

Huang

Geng

et al. 2011

Plant Foods Hum Nutr

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show abstract

“…Genistein is a small-molecule EGFR tyrosine kinase (TK) inhibitor, which can inhibit TK activation and subsequently lead to blockade of EGFR signaling pathway by competing with adenosine triphosphate (ATP) binding to the TK domain of the receptor, resulting in the suppression of cell proliferation and differentiation [12][13][14][15]. For this reason, EGFR inhibitors, such as genistein, always serve as agents for treating a variety of solid tumors.…”

Section: Introductionmentioning

confidence: 99%

Effects of Epidermal Growth Factor Receptor Inhibitor Genistein on Proliferative Cholangitis in Rats

Jiang¹,

Jiang²,

Yan³

et al. 2010

Journal of Surgical Research

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Section: Introductionmentioning

confidence: 99%

“…The incidence of these tumors has been reported to be as high as 77% based on pathological specimens (3,4). Fibroids are noncancerous growths that develop in the muscular wall of the uterus, and they cause health problems such as pelvic pain, menorrhagia, and infertility (1,4). Etiology of fibroids is not well understood, although studies have shown that these tumors are hormonally dependent, similar to breast and ovarian cancers (4,5).…”

Section: Introductionmentioning

confidence: 99%

“…Fibroids are noncancerous growths that develop in the muscular wall of the uterus, and they cause health problems such as pelvic pain, menorrhagia, and infertility (1,4). Etiology of fibroids is not well understood, although studies have shown that these tumors are hormonally dependent, similar to breast and ovarian cancers (4,5). Treatment options depend on the size and location of the tumor, patient's symptoms, age, and future reproductive plans.…”

Section: Introductionmentioning

confidence: 99%

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Genistein Suppresses Spontaneous Oviduct Tumorigenesis in Quail

Şahin

Akdemir

Tuzcu

et al. 2009

Nutrition and Cancer

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Spontaneous leiomyomas of the oviduct are common tumors of the Japanese quail (Coturnix coturnix japonica) and laying hens. This makes it a good animal model for screening potential agents for testing in the prevention and treatment of human myoma uteri. Genistein has been shown to inhibit the growth of various cancer cells. We investigated the effects of genistein supplementation on the development of fibroid tumors in the oviduct, serum oxidative stress markers [malondialdehyde (MDA), 8-isoprostane, 4-hydroxyalkenal (HAE), 8-hydroxy-2' -deoxyguanosine (8-OHdG) levels], soy isoflavone levels, and tissue biomarkers [Connexin 43 (Cx43), Bcl-2, and Bax and heat shock protein 70 (Hsp70) expression] in Japanese quail. One hundred and fifty quail (12 mo old) were assigned to 3 experimental groups as 5 replicates of pens containing 10 birds in each. Birds were fed either a basal diet or the basal diet supplemented with 400 mg or 800 mg of genistein/kg of diet. The animals were sacrificed after 315 days, and the tumors were identified. Genistein supplementation significantly decreased the incidence of fibroid tumors as compared to control birds (P = 0.04). The tumors in genistein-fed birds were smaller than those found in control birds (P = 0.02). Serum MDA, 8-isoprostane, and HAE levels were lower in treatment groups than in control group (MDA: 2.01 vs. 0.82; 8-isoprostane: 135 vs. 101; HAE: 1.45 vs. 0.73; P

show abstract

Stimulatory and inhibitory effects of genistein on human uterine leiomyoma cell proliferation are influenced by the concentration

Cited by 53 publications

References 48 publications

Soy-derived Isoflavones Inhibit HeLa Cell Growth by Inducing Apoptosis

Soy-derived Isoflavones Inhibit HeLa Cell Growth by Inducing Apoptosis

Effects of Epidermal Growth Factor Receptor Inhibitor Genistein on Proliferative Cholangitis in Rats

Genistein Suppresses Spontaneous Oviduct Tumorigenesis in Quail

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