Stimulative effects of lead on bone resorption in organ culture

Miyahara, Tatsuro; Komiyama, Hiroko; Miyanishi, A.; Takata, Masasuke; Nagai, Miyuki; Kono, Hiroshi; Hayashi, Toshimitsu; Yamamoto, Masahide; Ito, Yusuke; Odake, Hiroshi; Koizumi, Fumitomo

doi:10.1016/0300-483x(94)02948-t

Cited by 17 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Pb has been shown to alter the Ca homeostasis and perturb the cellular metabolism or activity of osteoclasts [86] and osteoblasts [87–92]. As already stated Pb 2 + has a much higher affinity to osteocalcin than Ca 2 + [45] and as a consequence Pb 2 + influences the binding properties of osteocalcin to the bone minerals negatively [44].…”

Section: Discussionmentioning

confidence: 99%

Spatial distribution of the trace elements zinc, strontium and lead in human bone tissue

Pemmer

Roschger

Wastl

et al. 2013

Bone

155

111

View full text Add to dashboard Cite

Trace elements are chemical elements in minute quantities, which are known to accumulate in the bone. Cortical and trabecular bones consist of bone structural units (BSUs) such as osteons and bone packets of different mineral content and are separated by cement lines. Previous studies investigating trace elements in bone lacked resolution and therefore very little is known about the local concentration of zinc (Zn), strontium (Sr) and lead (Pb) in BSUs of human bone. We used synchrotron radiation induced micro X-ray fluorescence analysis (SR μ-XRF) in combination with quantitative backscattered electron imaging (qBEI) to determine the distribution and accumulation of Zn, Sr, and Pb in human bone tissue.Fourteen human bone samples (10 femoral necks and 4 femoral heads) from individuals with osteoporotic femoral neck fractures as well as from healthy individuals were analyzed. Fluorescence intensity maps were matched with BE images and correlated with calcium (Ca) content. We found that Zn and Pb had significantly increased levels in the cement lines of all samples compared to the surrounding mineralized bone matrix. Pb and Sr levels were found to be correlated with the degree of mineralization. Interestingly, Zn intensities had no correlation with Ca levels. We have shown for the first time that there is a differential accumulation of the trace elements Zn, Pb and Sr in BSUs of human bone indicating different mechanisms of accumulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Spatial distribution of the trace elements zinc, strontium and lead in human bone tissue

Pemmer

Roschger

Wastl

et al. 2013

Bone

155

111

View full text Add to dashboard Cite

show abstract

“…Lead may affect both the osteoblast and the osteoclast. In organ culture lead stimulated radiolabeled calcium release [15] and enhanced the production of osteoclast-like multinucleated cells in bone marrow culture by a mechanism involving increases in PGE 2 , cAMP and calcium ions [42]. In addition, we have previously shown that lead increased intracellular calcium in osteoblasts [43].…”

Section: Discussionmentioning

confidence: 99%

“…Lead suppressed type II and type X collagen expression in chondrocytes and altered growth factors and second messenger signaling responses during chondrocyte maturation [13,14]. In osteoclasts lead stimulated bone resorption [15]. …”

Section: Introductionmentioning

confidence: 99%

The effect of lead on bone mineral properties from female adult C57/BL6 mice

Monir

Gundberg

Yagerman

et al. 2010

Bone

View full text Add to dashboard Cite

Lead toxicity is a significant problem in the U.S. with elevated blood lead levels being highest among very young children and older adults > 50 years old. Bone is the major reservoir of body lead, accounting for 75% in children and 90% in adults. Very little is known about the effect of lead on bone mineral properties in adults. We investigated the effect of lead on the femora from adult, 6 month old female C57/BL6 mice who were administered lead in the drinking water (250 ppm, blood lead 33 μg/dl) for 4 months. Bone mineral properties were examined using Fourier Transform Infrared Microscopy (FTIRM), quantitative microcomputed tomography (microCT) and whole bone mechanical testing. Lead significantly decreased the bone mineral density in the cortical and proximal cancellous bone and increased the marrow area in the cortical bone with microCT. Whole bone three-point bending showed a trend of decreased maximum and failure moments in the lead treated bones compared to controls. Lead significantly decreased the mineral/matrix ratio, collagen maturity and crystallinity in the trabecular bone as measured by FTIRM. In the cortical bone lead significantly decreased collagen maturity and bone crystal size by FTIRM. In contrast to cell culture studies, lead significantly increased serum osteocalcin levels. Lead also significantly increased the bone formation and resorption markers suggesting increased bone turnover. These data show lead increases bone turnover resulting in weaker cortical bone in adult female mice and suggest that lead may exacerbate bone loss and osteoporosis in the elderly.

show abstract

“…Impairment of bone matrix is mostly caused by interference with the collagen and noncollagen protein synthesis (11,12). Lead also displaces calcium ions from synthesized osteocalcin, which in turn inhibits its binding to hydroxyapatite, disturbing bone formation (13,14). In vitro , lead stimulates the proliferation of osteoclast‐like cells from the bone marrow of rats, and also the production of prostaglandin E2 (15).…”

mentioning

confidence: 99%