Stimulative effects of lead on bone resorption in organ culture

The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells

“…This extracellular concentration is physiologically relevant as such levels may be found in the blood (Goyer, 1993). Higher levels which could be reached during chronic Pb 2+ exposure or pregnancy may lead to osteosclerosis, malformation, or enhanced bone resorption in adults (Pounds and Cory-Slechta, 1993;Goyer et al, 1994;Miyahara et al, 1995), whereas children may suffer from minor growth and/or reduction of chest circumference (Dowd et al, 1994). Extracellular Pb 2+ displaces Ca 2+ from osteocalcin binding sites (Dowd et al, 1994) and interferes with the secretion of osteonectin (Sauk et al, 1992), both of which may change the bone matrix.…”

The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells

“…This extracellular concentration is physiologically relevant as such levels may be found in the blood (Goyer, 1993). Higher levels which could be reached during chronic Pb 2+ exposure or pregnancy may lead to osteosclerosis, malformation, or enhanced bone resorption in adults (Pounds and Cory-Slechta, 1993;Goyer et al, 1994;Miyahara et al, 1995), whereas children may suffer from minor growth and/or reduction of chest circumference (Dowd et al, 1994). Extracellular Pb 2+ displaces Ca 2+ from osteocalcin binding sites (Dowd et al, 1994) and interferes with the secretion of osteonectin (Sauk et al, 1992), both of which may change the bone matrix.…”

Spatial distribution of the trace elements zinc, strontium and lead in human bone tissue

“…For example, Pb has been shown to alter the Ca homeostasis and perturb the cellular metabolism or activity of osteoclasts [86] and osteoblasts [87–92]. As already stated Pb 2 + has a much higher affinity to osteocalcin than Ca 2 + [45] and as a consequence Pb 2 + influences the binding properties of osteocalcin to the bone minerals negatively [44].…”

The effect of lead on bone mineral properties from female adult C57/BL6 mice

“…Lead may affect both the osteoblast and the osteoclast. In organ culture lead stimulated radiolabeled calcium release [15] and enhanced the production of osteoclast-like multinucleated cells in bone marrow culture by a mechanism involving increases in PGE 2 , cAMP and calcium ions [42]. In addition, we have previously shown that lead increased intracellular calcium in osteoblasts [43].…”

“…Lead suppressed type II and type X collagen expression in chondrocytes and altered growth factors and second messenger signaling responses during chondrocyte maturation [13,14]. In osteoclasts lead stimulated bone resorption [15]. …”

The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells

“…This extracellular concentration is physiologically relevant as such levels may be found in the blood (Goyer, 1993). Higher levels which could be reached during chronic Pb 2+ exposure or pregnancy may lead to osteosclerosis, malformation, or enhanced bone resorption in adults (Pounds and Cory-Slechta, 1993;Goyer et al, 1994;Miyahara et al, 1995), whereas children may suffer from minor growth and/or reduction of chest circumference (Dowd et al, 1994). Extracellular Pb 2+ displaces Ca 2+ from osteocalcin binding sites (Dowd et al, 1994) and interferes with the secretion of osteonectin (Sauk et al, 1992), both of which may change the bone matrix.…”

Spatial distribution of the trace elements zinc, strontium and lead in human bone tissue

“…For example, Pb has been shown to alter the Ca homeostasis and perturb the cellular metabolism or activity of osteoclasts [86] and osteoblasts [87–92]. As already stated Pb 2 + has a much higher affinity to osteocalcin than Ca 2 + [45] and as a consequence Pb 2 + influences the binding properties of osteocalcin to the bone minerals negatively [44].…”

The effect of lead on bone mineral properties from female adult C57/BL6 mice

“…Lead may affect both the osteoblast and the osteoclast. In organ culture lead stimulated radiolabeled calcium release [15] and enhanced the production of osteoclast-like multinucleated cells in bone marrow culture by a mechanism involving increases in PGE 2 , cAMP and calcium ions [42]. In addition, we have previously shown that lead increased intracellular calcium in osteoblasts [43].…”

“…Lead suppressed type II and type X collagen expression in chondrocytes and altered growth factors and second messenger signaling responses during chondrocyte maturation [13,14]. In osteoclasts lead stimulated bone resorption [15]. …”

The effect of lead on calcium release activated calcium influx in primary cultures of human osteoblast-like cells

“…This extracellular concentration is physiologically relevant as such levels may be found in the blood (Goyer, 1993). Higher levels which could be reached during chronic Pb 2+ exposure or pregnancy may lead to osteosclerosis, malformation, or enhanced bone resorption in adults (Pounds and Cory-Slechta, 1993;Goyer et al, 1994;Miyahara et al, 1995), whereas children may suffer from minor growth and/or reduction of chest circumference (Dowd et al, 1994). Extracellular Pb 2+ displaces Ca 2+ from osteocalcin binding sites (Dowd et al, 1994) and interferes with the secretion of osteonectin (Sauk et al, 1992), both of which may change the bone matrix.…”

Spatial distribution of the trace elements zinc, strontium and lead in human bone tissue

“…For example, Pb has been shown to alter the Ca homeostasis and perturb the cellular metabolism or activity of osteoclasts [86] and osteoblasts [87–92]. As already stated Pb 2 + has a much higher affinity to osteocalcin than Ca 2 + [45] and as a consequence Pb 2 + influences the binding properties of osteocalcin to the bone minerals negatively [44].…”

The effect of lead on bone mineral properties from female adult C57/BL6 mice

“…Lead may affect both the osteoblast and the osteoclast. In organ culture lead stimulated radiolabeled calcium release [15] and enhanced the production of osteoclast-like multinucleated cells in bone marrow culture by a mechanism involving increases in PGE 2 , cAMP and calcium ions [42]. In addition, we have previously shown that lead increased intracellular calcium in osteoblasts [43].…”

“…Lead suppressed type II and type X collagen expression in chondrocytes and altered growth factors and second messenger signaling responses during chondrocyte maturation [13,14]. In osteoclasts lead stimulated bone resorption [15]. …”