Stimulation with THBS4 activates pathways that regulate proliferation, migration and inflammation in primary human keratinocytes

Mäemets-Allas, Kristina; Klaas, Mariliis; Cárdenas-León, Claudia Griselda; Arak, Terje; Kankuri, Esko; Jaks, Viljar

doi:10.1016/j.bbrc.2022.12.052

Cited by 8 publications

(6 citation statements)

References 51 publications

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“…THBS4, a member of the thrombospondin family of ECM proteins, was also detected in our stromal proteome and showed higher expression in the 14-day than in the 7-day preserved stroma. THBS4 not only plays a role in tissue remodeling, inflammation, and angiogenesis but also is recognized for its involvement in promoting wound healing, stimulating keratinocyte proliferation, and enhancing the migration of primary human keratinocytes . Alternatively, thrombospondin-1 (THBS1 or TSP-1), another member of the thrombospondin family, was also detected in our stroma proteome analysis with similar expression levels during the 7- and 14-day preservation periods (Supplementary Table S2).…”

Section: Resultsmentioning

confidence: 81%

“…THBS4 not only plays a role in tissue remodeling, inflammation, and angiogenesis 29 but also is recognized for its involvement in promoting wound healing, stimulating keratinocyte proliferation, and enhancing the migration of primary human keratinocytes. 30 Alternatively, thrombospondin-1 (THBS1 or TSP-1), another member of the thrombospondin family, was also detected in our stroma proteome analysis with similar expression levels during the 7and 14-day preservation periods (Supplementary Table S2). It serves as a major activator of latent TGF-β and supports TGFβ-mediated immune responses and wound healing functions.…”

Section: Characterization Of Human Corneal Stroma Proteomementioning

confidence: 73%

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Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma

Wu,

Liu,

Wong

et al. 2023

J. Proteome Res.

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Cornea transplantation is one of the most commonly performed allotransplantations worldwide. Prolonged storage of donor corneas leads to decreased endothelial cell viability, severe stromal edema, and opacification, significantly compromising the success rate of corneal transplantation. Corneal stroma, which constitutes the majority of the cornea, plays a crucial role in maintaining its shape and transparency. In this study, we conducted proteomic analysis of corneal stroma preserved in Optisol-GS medium at 4 °C for 7 or 14 days to investigate molecular changes during storage. Among 1923 identified proteins, 1634 were quantifiable and 387 were significantly regulated with longer preservation. Compared to stroma preserved for 7 days, proteins involved in ocular surface immunomodulation were largely downregulated while proteins associated with extracellular matrix reorganization and fibrosis were upregulated in those preserved for 14 days. The increase in extracellular matrix structural proteins together with upregulation of growth factor signaling implies the occurrence of stromal fibrosis, which may compromise tissue clarity and cause vision impairments. This study is the first to provide insights into how storage duration affects corneal stroma from a proteomic perspective. Our findings may contribute to future research efforts aimed at developing long-term preservation techniques and improving the quality of preserved corneas, thus maximizing their clinical application.

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Section: Resultsmentioning

confidence: 81%

Section: Characterization Of Human Corneal Stroma Proteomementioning

confidence: 73%

Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma

Wu,

Liu,

Wong

et al. 2023

J. Proteome Res.

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“…Nectin-3 is a cell–cell adhesion molecule that plays an important role in the formation of cell–cell adhesion junctions in keratinocytes ( Tanaka et al 2003 ) and is required for cell adhesion and differentiation in hair follicles and for proper hair formation in mice ( Yoshida et al 2014 ). Thrombospondins are extracellular glycoproteins participate in cell–cell and cell–extracellular matrix interaction ( Adams and Lawler 2004 , 2011 ) and are important for skin keratinocyte development ( Adams and Lawler 2004 , 2011 ; Mäemets-Allas et al 2023 ). In particular, THBS4 orthologs are related to skin pigmentation in pearl danio ( Danio albolineatus ) and may play a general role in body color development across vertebrates ( Huang et al 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Genetic Basis and Evolution of Structural Color Polymorphism in an Australian Songbird

Sin,

Ke,

Chen

et al. 2024

Molecular Biology and Evolution

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Island organisms often evolve phenotypes divergent from their mainland counterparts, providing a useful system for studying adaptation under differential selection. In the white-winged fairywren (Malurus leucopterus), subspecies on two islands have a black nuptial plumage whereas the subspecies on the Australian mainland has a blue nuptial plumage. The black subspecies have a feather nanostructure that could in principle produce a blue structural color, suggesting a blue ancestor. An earlier study proposed independent evolution of melanism on the islands based on the history of subspecies divergence. However, the genetic basis of melanism and the origin of color differentiation in this group are still unknown. Here, we used whole-genome resequencing to investigate the genetic basis of melanism by comparing the blue and black M. leucopterus subspecies to identify highly divergent genomic regions. We identified a well-known pigmentation gene ASIP and four candidate genes that may contribute to feather nanostructure development. Contrary to the prediction of convergent evolution of island melanism, we detected signatures of a selective sweep in genomic regions containing ASIP and SCUBE2 not in the black subspecies but in the blue subspecies, which possesses many derived SNPs in these regions, suggesting that the mainland subspecies has re-evolved a blue plumage from a black ancestor. This proposed re-evolution was likely driven by a pre-existing female preference. Our findings provide new insight into the evolution of plumage coloration in island versus continental populations, and, importantly, we identify candidate genes that likely play roles in the development and evolution of feather structural coloration.

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“…Thrombospondins are extracellular glycoproteins participate in cell-cell and cell-extracellular matrix interaction 53,54 . Both THBS genes are important for skin keratinocyte development [53][54][55] . These three genes are only divergent between DHI and mainland populations but not between BI and mainland populations (Fig.…”

Section: Genetic Basis Of Plumage Color Difference In M Leucopterusmentioning

confidence: 99%

Genetic basis and evolution of structural color polymorphism in an Australian songbird

Sin,

Ke,

Chen

et al. 2023

Preprint

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Island organisms often evolve phenotypes divergent from their mainland counterparts, providing a useful system for studying adaption under differential selection. Some island birds have melanic plumage differing from the color of mainland conspecifics, a trait proposed as an insular adaptation. In the white-winged fairywren (Malurus leucopterus), subspecies on two islands have a black nuptial plumage whereas the subspecies on the Australian mainland has a blue nuptial plumage. The black subspecies have a feather nanostructure that could produce a blue structural color, suggesting a blue ancestor. An earlier study proposed independent evolution of melanism on the islands based on the history of subspecies divergence. However, the genetic basis of melanism and the origin of color differentiation in this group are still unknown. Here, we used whole-genomes to investigate the genetic basis of melanism by comparing the blue and black M. leucopterus subspecies to identify highly divergent genomic regions. We identified a well-known pigmentation gene ASIP and four candidate genes that may contribute to feather nanostructure development. We also detected signatures of a selective sweep in genomic regions containing ASIP and SCUBE2 not in the black subspecies, as predicted by earlier work, but in the blue subspecies, which possesses many derived SNPs in these regions, suggesting that the mainland subspecies has re-evolved a blue plumage from a black ancestor. This re-evolution was likely driven by a pre-existing female preference. Our findings provide new insight into the evolution of plumage coloration in island versus continental populations, and, importantly, we identify candidate genes that likely play roles in the development and evolution of feather structural coloration.

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Stimulation with THBS4 activates pathways that regulate proliferation, migration and inflammation in primary human keratinocytes

Cited by 8 publications

References 51 publications

Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma

Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma

Genetic Basis and Evolution of Structural Color Polymorphism in an Australian Songbird

Genetic basis and evolution of structural color polymorphism in an Australian songbird

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