Stimulation of Uridine Incorporation in Isolated Bone Cells by Parathyroid Hormone and Cyclic AMP<sup>1</sup><sup>2</sup>

Peck, William A.; Messinger, Kirk; Kimmich, George A.; Carpenter, J. G.

doi:10.1210/endo-95-1-289

Cited by 16 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years the action of cyclic AMP on RNA synthesis was demonstrated in different tissues, including adrenocortical cells (Nussdorfer 8c Mazzochi 1972), neuroblastoma cells (Prasad et al 1973), ovary (Jarlstedt et al 1973), and bone (Peck et al 1974). The present results demonstrate that cyclic GMP stimulates the incorporation of [3H] uridine into RNA, thus providing a possible site of action which could explain the effect of this nucleotide on in vivo thyroid protein biosynthesis (Pisarev et al 1971).…”

Section: Resultssupporting

confidence: 59%

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Pisarev

1977

Acta Endocrinologica

View full text Add to dashboard Cite

Thyrotrophin (TSH) regulates the biosynthesis of thyroid protein and RNA. This action is mediated by adenylate cyclase and cyclic AMP. In the present study the action of cyclic GMP and cyclic CMP was investigated in beef slices. Both cyclic AMP and cyclic GMP significantly increased the incorporation of [3H]uridine into RNA. These effects were blocked by actinomycin D, suggesting that their action is located at a preor at a transcriptional step. The PCA soluble fraction radioactivity followed in parallel with the variations observed in the RNA fraction, supporting the view that cyclic nucleotides may regulate RNA by modulating the nucleotide precursors pool. * Established investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.Abbreviations: TSH: thyrotrophin; TCA: trichloroacetic acid: PCA: perchloric acid; cyclic AMP, GMP and CMP stand for the 3'5' cyclic monophosphate compounds of adenosine, guanosine and cytidine, respectively.

show abstract

Section: Resultssupporting

confidence: 59%

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Pisarev

1977

Acta Endocrinologica

View full text Add to dashboard Cite

show abstract

“…Cells treated with bovine parathyroid extract (PTE) and DBcAMP but not SCT grew abundantly. Because of the limited supply of cells available for stud}', this effect was studied quantitatively with tritiated thymidine and tritiated uridine using PTE only [17,18]. Twenty-four wells were seeded with either pagetic cells or Vero cells (continuous line of monkey kidney fibroblasts, ATTCI.…”

Section: Hormonal Stimula Ti+mmentioning

confidence: 99%

Long-term culture of cells from bone affected by Paget’s disease

et al. 1979

View full text Add to dashboard Cite

Cells obtained from surgical bone specimens of eight patients with Paget's disease of bone were maintained in culture for up to 8 months and seven passages. The doubling time during the period of maximal cell growth ranged from 4 to 12 days. Evidence consistent with the hypothesis that many of the cells were bone cells included the following: (a) histochemical techniques demonstrated staining of some cells for alkaline phosphatase or acid phosphatase and succinic dehydrogenase; (b) parathyroid extract stimulated increased uptake of 3H-thymidine and 3H-uridine; (c) parathyroid extract suppressed and salmon calcitonin stimulated uptake of 3H-proline; and (d) crystalline calcium deposits were found within cells and extracellularly. Ultrastructural analysis revealed that three of the eight cultures contained cells whose nuclei had inclusions which were almost identical to those found in the osteoclast nuclei of all patients with Paget's disease. The maintenance of cells derived from pagetic bone in long-term culture should aid in testing the hypothesis that Paget's disease represents a slow virus infection of bone.

show abstract

“…Control studies with adenosine and 5'-AMP indicated the specificity of this effect of the cyclic nucleotide (in preparation). Peck et al (1974) have recently shown that cyclic AMP stimulated labelled uridine incorporation into RNA in isolated bone cells and increased PCA-soluble radioactivity. As discussed earlier, the effects on the PCA-soluble fraction would suggest an effect at the precursor pool level.…”

Section: Resultsmentioning

confidence: 99%

“…The latter fraction is known to contain uridine and the phosphorylated nucleotides (UMP, UDP and UTP) (Lamy et al 1971;Peck et al 1974). Therefore our findings would indicate that cyclic AMP and KI control RNA synthesis at the step of phosphorylation of nucleotides.…”

Section: Resultsmentioning

confidence: 99%

Action of Ki, Thyroxine and Cyclic Amp on [3h]uridine Incorporation Into the Rna of Thyroid Slices

Pisarev¹,

Aiello²,

Pisarev³

1976

Acta Endocrinologica

View full text Add to dashboard Cite

Potassium iodide (KI) has been shown to impair thyroid protein biosynthesis both in vivo and in vitro. The present study was performed in order to clarify its mechanism of action. Ribonucleic acid (RNA) synthesis was studied in beef thyroid slices with either [32P] or [ 3H] \ x=req-\ uridine as labelled precursors. Both KI and thyroxine (T4) at 10\m=-\5 m significantly decreased RNA labelling under our conditions. In other experiments RNA degradation was examined in pulse-labelled and actinomycin D-treated slices. KI did not modify the degradation of the [ 3H] \ x=req-\ RNA thus indicating that it interferes with the biosynthesis rather than with the degradation of RNA. Taking the perchloric acid soluble radioactivity as a rough index of the precursor pool the present results would indicate an action at this level. Both KClO4 and methylmercapto-imidazole relieved the gland from the inhibitory action of KI, supporting the view that an intracellular and

show abstract

Stimulation of Uridine Incorporation in Isolated Bone Cells by Parathyroid Hormone and Cyclic AMP¹²

Cited by 16 publications

References 0 publications

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Long-term culture of cells from bone affected by Paget’s disease

Action of Ki, Thyroxine and Cyclic Amp on [3h]uridine Incorporation Into the Rna of Thyroid Slices

Contact Info

Product

Resources

About

Stimulation of Uridine Incorporation in Isolated Bone Cells by Parathyroid Hormone and Cyclic AMP12

Cited by 16 publications

References 0 publications

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Action of Cyclic Nucleotides on Protein and Rna Synthesis in the Thyroid

Long-term culture of cells from bone affected by Paget’s disease

Action of Ki, Thyroxine and Cyclic Amp on [3h]uridine Incorporation Into the Rna of Thyroid Slices

Contact Info

Product

Resources

About

Stimulation of Uridine Incorporation in Isolated Bone Cells by Parathyroid Hormone and Cyclic AMP¹²