Stimulation of the DNA-synthesizing enzymes of cultured human cells by vaccinia virus infection

The Effect of p-Fluorophenylalanine on the Replication of Rabbitpox Virus and its Nucleic Acid

“…It is known that the growth of vaccinia virus causes increased activity of at least two enzymes (thymidine kinase, DNA polymerase), which is first detectable in cells about 2 hr after infection (Green & Pina, 1962 Kit, Dubbs & Piekarski, 1963). It is therefore likely that the inhibition of DNA synthesis by FPA in our experiments was due to interference with the formation of these or similar enzymes.…”

Proflavine Inhibition of Vaccinia Virus Synthesis

“…It is significant that an eclipse period, characteristic for vaccinia virus, followed reversal of proflavine inhibition. This finding again suggests that proflavine may block an early stage in the infectious cycle, e.g., interference with the viral uncoating process or the synthesis of enzymes associated with viral DNA replication (Magee, 1962;Green and Pina, 1962).…”

“…The selective photosensitization of vaccinia early change occurring in vaccinia-infected cells virus during the early stages of virus synthesis is the stimulation of DNA-synthesizing enzymes (Fig. 4) suggested that proflavine interacted with (Green and Pina, 1962;Magee, 1962), and in this vaccinia DNA while this material existed in a regard it is significant that proflavine inhibits the naked form or in loose complex with other virus enzymatic synthesis of DNA and RNA (Hurwitz precursors. Proflavine-DNA interaction coincided et al, 1962).…”

Activity of Aspartate Transcarbamylase in Uninfected and Type 5 Adenovirus-Infected Hela Cells

“…Infection of E. coli with T-even bacteriophages is followed by the appearance of a number of new enzymes, as well as an increase in activity of a number of constitutive cell enzymes (Cohen, 1961). Infection of mammalian cells with vaccinia, herpes simplex, or Mengo virus has been shown to initiate increased activity of some enzymes concerned with nucleic acid biosynthesis: thymidine and thymidvlate kinases, DNA polymerase, RNA polymerase, and deoxyribonuclease have been measured (Hanafusa, 1961;Baltimore and Franklin, 1962;Magee, 1962;Green and Pina, 1962;McAuslan and Joklik, 1962;Kit, Dubbs, and Piekarski, 1962;Piekarski, and Dubbs, 1963;. To date, the enzymes studied have not been demonstrated to be different from those in uninfected cells, except for the evidence that herpes simplex virus infection of a mutant strain of L cells, strain LM, initiates production of thymidine kinase in cells in which the enzyme cannot be detected in the absence of virus infection .…”