Stimulation of the development of bovine embryos by insulin and insulin-like growth factor-I (IGF-I) is mediated through the IGF-I receptor

Matsui, Motozumi; Takahashi, Yoshiyuki; Hishinuma, Mitsugu; Kanagawa, Hiroshi

doi:10.1016/s0093-691x(97)00277-x

Cited by 38 publications

(25 citation statements)

References 37 publications

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“…A threshold may exist at which the level of growth hormone, IGF-I or insulin goes from being beneficial to detrimental on oocyte and embryo development. Matsui et al (1997) reported that insulin at concentrations of 0.5 -10 mg/ml had a beneficial effect on the rate of development to the morula stage on day 5 of embryo culture and that this stimulation of embryonic development was mediated through the IGF-I receptor. However, these authors did not report the frequency of development to the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Fouladi-Nashta

Campbell²

2006

Reproduction

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Follicles of 4-8 mm in diameter were dissected from ovaries and cultured in Waymouth culture medium in the presence or absence of insulin (5 mg/ml) at 39 8C in a humidified atmosphere of 45% O 2 , 5% CO 2 and 50% N 2 for 24 h. Following follicle culture, the oocytes were collected and examined for developmental potential, total protein profile and ultrastructural aspects. Oocytes aspirated directly from follicles of the same size were used as controls. Addition of insulin to the follicle culture medium significantly reduced expression of the low molecular weight insulin-like growth factor-binding proteins (IGFBPs) in the follicular fluid, and significantly reduced the cleavage rate of subsequently matured and fertilised oocytes (0.52 vs 0.61). However, there were no differences in the proportion of cleaved embryos which developed to the blastocyst stage (0.30 vs 0.28), nor embryo quality as assessed by total cell number (137 6 8.53 vs124.6 6 6.95). The total protein profiles of immature oocytes recovered after 24 h of follicle culture were compared by PAGE. There were marked differences between the two groups, unmatured oocytes recovered from the insulin-positive follicle group showed a protein pattern similar to that of matured oocytes. In addition, examination of ultrastructural features by transmission electron microscopy indicated that oocytes from follicles cultured in the presence of insulin undergo many of the cytoplasmic changes associated with oocyte maturation. In conclusion, follicle culture in the presence of insulin is beneficial for follicular survival and significantly reduces cleavage but has no detrimental effects on the development of cultured embryos. However, many of the cytoplasmic changes associated with oocyte maturation occur prior to the induction of nuclear maturation.

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Section: Discussionmentioning

confidence: 99%

Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Fouladi-Nashta

Campbell²

2006

Reproduction

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“…Ž . Matsui et al, 1995Matsui et al, , 1997 , a combination of TGFa and bFGF Larson et al, 1992a , Ž . Ž .…”

Section: Discussionmentioning

confidence: 99%

“…Keskintepe et al, 1995;Takahashi et al, 1996;Matsui et al, 1997 , protein supplemen-Ž tation has been shown to be beneficial for embryo development in vitro Takahashi and . First, 1992;Carolan et al, 1995 .…”

Section: Introductionmentioning

confidence: 99%

Effects of glucose and protein sources on bovine embryo development in vitro

Gómez

Díez

2000

Animal Reproduction Science

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Oviductal factors may be obtained by ultrafiltration of conditioned medium, added to a simple media and used in bovine embryo culture. In this study, we aimed to analyze the development of bovine embryos produced with oviductal factors compared to those cultured in the presence of BSA or serum, the effects of glucose in presence of these protein supplements, and the ability of oviductal factors to support embryo development during the entire culture period. In vitro produced bovine zygotes from slaughterhouse ovaries were cultured in modified-synthetic oviduct Ž . Ž . Ž . Ž . fluid mSOF alone or supplemented with 1 oviductal factors, 2 BSA and 3 FCS. Oviductal factors showed embryotrophic activity, although with blastocyst rates lower than those in BSA and Ž . FCS. Glucose 1.5 mM added at Day 2 of culture did not affect development in the presence of oviductal factors. The number of cells in expanded blastocysts was unaffected by the presence of glucose or any of the protein supplements used. Both BSA and FCS, respectively, improved blastocyst rates of Day 6 embryos produced with oviductal factors. The effect of oviductal factors was masked by the presence of BSA during the entire culture. FCS promoted an earlier appearance of blastocysts. It is concluded that the effect of glucose on in vitro embryo development depends upon the source of protein. Oviductal factors are not an appropriate supplement for embryos beyond Day 6 of culture in SOF, although blastocyst rates of such embryos may be increased by culturing them in the presence of FCS or BSA. q

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“…Actions of IGF1 on the preimplantation embryo indicate that both MAPK and PI3K/AKT pathways are activated (Figure 2). Culture with IGF1 increases the percent of embryos capable of developing to the blastocyst stage (28,30,(64)(65)(66)(67)(68)(69). This action of IGF1 probably involves increased proliferation because the cell number of day 6 morulae was increased by IGF1 (70).…”

Section: Insulin-like Growth Factormentioning

confidence: 99%

“…This action of IGF1 probably involves increased proliferation because the cell number of day 6 morulae was increased by IGF1 (70). Effects on development could be blocked with antibody to IGF1R (64) and an inhibitor of MAPK activation (69,70). IGF1 also blocks activation of apoptosis caused by exposure to heat shock (70,71) or the prooxidant menadione (72).…”

Section: Insulin-like Growth Factormentioning

confidence: 99%

Maternal embryokines that regulate development of the bovine preimplantation embryo

Hansen¹,

Denicol²,

Dobbs³

2014

Turk J Vet Anim Sci

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The zygote contains within its genome and epigenome the program to direct development through the embryonic, fetal, and postnatal periods. The execution of that program is dependent on the embryo's nongenetic inheritance from the oocyte and sperm (1,2) as well as on the environment in which development proceeds. Variation in the maternal environment can affect the ability of the preimplantation embryo to establish pregnancy. In cattle, the focus of this review, competence of the preimplantation embryo for growth and survival can be affected by maternal parity (3), lactational status (4), and circulating concentrations of progesterone (5). Moreover, patterns of gene expression in the endometrium are associated with survival of an embryo transferred to the uterus in the next ovulatory cycle (6,7).Development of the bovine embryo to the blastocyst in the absence of maternal signals (i.e. through in vitro production procedures) results in embryos with aberrant gene expression (8,9), lipid content (10,11), DNA methylation (12), and, as shown in the Table, reduced competence to establish pregnancy after transfer into recipients (13-17). Additionally, an increased proportion of embryos produced in vitro have developmental abnormalities that lead to increased neonatal death losses (18-20). Some of the problems with the in vitro-produced embryo could result from selection of incompetent oocytes or inadequate oocyte maturation. However, the importance of the maternal environment during development is indicated by observations that transfer of in vitro-produced embryos to the oviduct after fertilization limits some of the abnormalities associated with in vitro production (9,21).One function of the reproductive tract is to secrete bioactive molecules that regulate the embryo, oviduct, or endometrium. Genes for 115 ligands expressed in the endometrium had the corresponding receptor gene expressed by the embryo (22). Regulatory molecules produced by the oviduct and endometrium, which include hormones, growth factors, and cytokines, are referred to as embryokines when they function to regulate embryonic growth and development (23).It is likely that some of the variation in the ability of the reproductive tract to support embryonic development represents variation in secretion of embryokines. Indeed, several genes that were overexpressed in the endometrium of cows that subsequently became pregnant after embryo transfer as compared to cows that did not establish pregnancy are potential embryokines. These include NGF,

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Stimulation of the development of bovine embryos by insulin and insulin-like growth factor-I (IGF-I) is mediated through the IGF-I receptor

Cited by 38 publications

References 37 publications

Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Effects of glucose and protein sources on bovine embryo development in vitro

Maternal embryokines that regulate development of the bovine preimplantation embryo

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