Stimulation of RNA polymerase 1 activity by 17β‐estradiol—receptor complex on chick liver nucleolar chromatin

Bieri-Bonniot, Françoise; Joss, Urs R.; Dierks-Ventling, Christa

doi:10.1016/0014-5793(77)80935-6

Cited by 9 publications

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References 17 publications

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“…Although it is not recognized as a classical target organ for the sex hormones as are, for example, the tissues of the female reproductive tract, the liver has been shown to contain proteins with certain characteristics of the oestrogen receptor (Chamness et al, 1975;Eisenfeld et al, 1976). There is as yet no compelling evidence, as there is for the avian liver (Bieri-Bonniot et al, 1977;Jost et al, 1978), for oestrogen-induced changes in transcription in the mammalian liver; nevertheless it is widely accepted that oestrogen administration to rats results in altered liver function, including increases in the concentration of certain plasma proteins, e.g. renin substrate (Menard et al, 1973;Eisenfeld et al, 1977).…”

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Nucleo-cytoplasmic relationships of oestrogen receptors in rat liver during the oestrous cycle and in response to administered natural and synthetic oestrogen

Marr¹,

White²,

Elder³

et al. 1980

Biochemical Journal

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Oestrogen receptors were measured in the cytosolic and purified nuclear fractions of rat liver. Both cytosolic and nuclear receptors bind oestrogen with high affinity (Kd = 1.47 and 2.28 nM respectively) and specificity similar to that of receptors in order oestrogen-target tissues such as the uterus. During the 4-day oestrous cycle the receptor content and distribution between cytosol and nucleus did not vary; in particular, the content of nuclear receptor did not appear to fluctuate in concert with known cyclic changes in the concentration of plasma oestrogen. Injection of 50 micrograms of oestradiol-17 beta or 10 micrograms of ethynyloestradiol resulted in a 4--6-fold increase in the nuclear receptor content, with a concomitant decrease in the unoccupied-receptor content of cytosol 1 h after injection. The nuclear receptors present after injection bind oestrogens with similar affinity (Kd = 2.78 nM) and specificity to receptors present in uninjected animals. The administration of lower doses of either oestrogen was less effective in producing increases in nuclear receptor content. Hence there is apparently substantial translocation of receptor to the nucleus in response to hyperphysiological doses of oestrogen, but not to the physiological changes in plasma oestrogen concentrations during the oestrous cycle. The response to exogenous oestrogens is discussed in relation to the clinical use of synthetic oestrogens and progestogens.

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confidence: 99%