Stimulation of proliferation in stationary primary cultures of monkey and rabbit aortic smooth muscle cells

Effect of high and low density lipoproteins on proliferation of cultured bovine vascular endothelial cells.

“…Cuiltuires ofbovine vascuilai-enidiotheliail cells were established from adu(itlt aortic arch as already dlescril)e(l (21)(22)(23) concen-tration to which cultures were exposed. After 6 d, anI 8-an(l 10-fold increase in cell density was observed for cultures exposed to 5% plasmia or serumn, respectively (Fig. 1).…”

“…PreviO0iS studies have shown that both LDL and HDL can interact specifically with vascular endothelial cells (2-5)2,3 and others halve shown that LDL could be mitogenic for vascular smnooth muscle cells and dermal fibroblasts when added to lipoprotein-deficient serum (LPDS) (6)(7)(8) or to serum from a beta lipoproteinemic subjects (9). Likewise, in the case of cells that have a limited ability to miake cholesterol de novo or in the case of cells maintained in the presence of compounds such as compactin, which totally inhibit their ability to mnsake cholesterol, additioni of LDL to the mediumn leads to resumiiption of cell proliferation (10).…”

Structural and functional changes of rhesus serum low density lipoproteins during cycles of diet-induced hypercholesterolemia.

“…Both indicators of cellular proliferation were evaluated as previously described. 21 Due to the considerable variability in primary culture systems which are mainly caused by differences in donor animals, the results of each experiment were expressed relative to the values of the normal monkey serum control group.…”

George Lyman Duff Memorial Lecture. Atherosclerosis: a problem of the biology of arterial wall cells and their interactions with blood components.

“…LDL may cause functional endothelial injury, as evidenced by both morphologic and physiologic alterations in the endothelium 4 " 6 Furthermore, Fless et al 104 and Fischer-Dzoga et al, 105 colleagues of Wissler, have reported that a special category of LDL obtained from hyperlipemic monkeys is uniquely atherogenic.…”