Stimulation of Pancreatic Lipase Activity by Saponins Isolated from Medicago sativa L.

Abstract

Saponins isolated from Medicago sativa L. appeared to stimulate lipolytic activity and did not influence amylolytic or proteolytic activity of Neopancreatinum (extract of pancreatic enzymes: lipase, proteases and amylases). Saponins isolated from the aerial part of Medicago sativa L. were demonstrated to stimulate lipase activity more effectively than saponins sepa rated from the root of this plant.When saponins were treated with 0.02 m HC1 at 37 °C for 1 hour their lipase stimulation ability did not change. The saponins stimulated Neopancreatinum lipolytic activity more in tensively in the presence of sodium cholate.

Chemical and Biological Activity of Triterpene Saponins from Medicago Species

“…Results showed that they stimulated lipolytic activity and did not influence the proteolytic and amylolytic activities of Neopancreatium, a mixture of porcine pancreatic enzymes such as trypsin, chimotrypsin, lipase and amylase. An increase of the stimulatory effects of saponins was observed when sodium cholate was added to the medium [150].…”

Phytochemical and pharmacological potential ofMedicago sativa: A review

“…Saponins isolated from the aerial parts of M. sativa have been shown to stimulate lipolytic activity without influencing amylolytic or proteolytic activity of NeoPancreatinum (Sroka et al, 1997).…”

Enhanced triterpene saponin biosynthesis and root nodulation in transgenic barrel medic (Medicago truncatula Gaertn.) expressing a novel β‐amyrin synthase (AsOXA1) gene

“…Saponins may also be involved in the modulation of interactions with symbionts and pathogens (Iturbe‐Ormaetxe et al ., 2003). More interestingly, these compounds have potentially useful pharmacological activities, such as anticholesterolaemic, haemolytic, vaccine adjuvant, immunostimulatory and anticarcinogenic properties (Malinow et al ., 1977; Carroll and Kurowska, 1995; Bader et al ., 1996; Sroka et al ., 1997; Fournier et al ., 1998; Marciani et al ., 2000; Park et al ., 2001; Gaidi et al ., 2002; Singuaroli, 2002). As a result of their chemical, physical and physiological characteristics, commercial products containing plant saponins are available and are used in the pharmaceutical, cosmetic and food industries (Tanaka et al ., 1996).…”