Stimulation of natural enzymes for germination of mimosa weed seeds to enhanced bioethanol production

Barley straw is a lignocellulose agricultural waste material that can be utilized as a raw material for ethanol production since it is easy to find, cheap, and has the potential to produce ethanol yield. This research aims to select the optimum pretreatment conditions to increase fermentable sugar production during enzymatic hydrolysis for bioethanol production. Initially, sulfuric acid at a concentration of 0.5, 1.0, 1.5, and 2.0% (v/v) pre-treated barley straw biomass was applied by autoclaving at 121℃ for 15 lb/in2 pressure for 15 min. Furthermore, acid-treated barley straw under the alkaline condition with calcium hydroxide concentrations of 1, 2, 3, and 4% (w/v) was autoclaved at 121℃ for 15 lb/in2 pressure for 15 min. Subsequently, the pretreatment of barley straw in the acetic and alkaline processes was compared. It was found that calcium hydroxide at a concentration of 2% (w/v) gave more sugar concentrations. Finally, combined acid and alkaline pretreatment with 2% of cellulase enzymatic hydrolysis had the highest total sugar concentration of 205.43 g/L and reducing sugar of 134.42 g/L, producing the highest ethanol yield (16.17 g/L) by 24 hours of fermentation.

Section: Effects Of Ethanol Fermentation By S Cerevisiaesupporting

confidence: 91%

Pretreatment of barley straw with acid and alkaline solutions to boost the efficiency of fermentable yield enzymatic degradation in the separated fermentation technique for ethanol production

Ratchawet

Chaiworn

2022

“…The optimization also included optimizing acid concentration, time, and reaction time to obtain maximum fermentable sugars (Galindo-Leva et al, 2016). The naturally occurring yeast can ferment most of the sugars in lignocellulosic biomass, which has been used in the present study to ferment the acid hydrolysate (Ramaraj et al, 2021;Palanisamy et al, 2021b). However, S. cerevisiae is most widely used for ethanol production from hexose sugars because of its high ethanol productivity and tolerance to ethanol and inhibitors, unable to ferment the pentose sugars (xylose), which are the second most abundant sugar present in the lignocellulosic biomass.…”

Section: Resultsmentioning

confidence: 99%

Alkali pretreatment and enzymatic saccharification of blue-green alga Nostochopsis lobatus for bioethanol production

Pradechboon

Junluthin

2022

Using various Sodium hydroxide (NaOH) concentrations (0, 1, 2, and 3%), we studied the alkaline pretreatment of microalgal biomass from the blue-green alga Nostochopsis lobatus in this work. In this pretreatment stage, the entrapped polysaccharides found within the microalgae cell walls are broken down to form fermentable subunits. The selected pretreatment procedure (2% NaOH) was then followed by enzymatic saccharification for the generation of bioethanol. This pretreatment step aims to release and break down entrapped polysaccharides in the microalgae cell walls into fermentable subunits. The best result of sugars after enzyme hydrolysis was obtained in total and reducing sugars 210.22 and 98.54 g/L, respectively. In addition, Saccharomyces cerevisiae was used to ferment the hydrolysate; the highest ethanol yield was 14 g/L by 48 hours of fermentation. The alkaline pretreatment method was a promising option for pretreating microalgal biomass for bioethanol production.

“…First, agricultural wastes are composed of four components that differ in their average composition: cellulose, extractives, hemicellulose and lignin (Mejica et al, 2022). It is important to accurately characterize the beginning biomass to determine the products' yields and characteristics (Ramaraj et al, 2021). Corn is the most widely grown crop in the world (Zea mays).…”

Section: Feedstock Characteristicsmentioning

confidence: 99%

Influence of nanoparticles inclusion on the production of bioethanol from corn stalks and leaves

Saetang¹,

Tipnee²

2022

Bioethanol is a viable alternative to petroleum-derived fossil fuels. It is renewable, low-cost, and the preferred fuel for most developing countries worldwide. Although it is possible to make bioethanol from corn stalks and leaves wastes, second-generation biofuels made from agricultural waste feedstocks represent a significant step forward. In the present research, nickel oxide (NiO) nanoparticles (NPs) were used as a biocatalyst to achieve maximum ethanol output. The pretreatment of 2% NaOH with NiO NPs, 15-min autoclave condition, showed the highest total and reducing sugar yield was 162.69 g/L and 43.75 g/L. After hydrolysis, the suitable total and reducing sugar yield of 185.43 g/L and 125.42 g/L was chosen for further fermentation with the expansion of Saccharomyces cerevisiae cells. Separate hydrolysis and fermentation (SHF) using corn stalk and leaf waste was significantly assisted by incorporating a nanocatalyst; ethanol concentration was increased to 15.8 g/L at 24 hours incubation period. The study revealed critical information regarding ways NiO NPs could be employed to improve the efficiency of the ethanol production bioprocess.