Stimulation of multiple shoot formation on soybean stem nodes in culture

Somaclonal variation — a novel source of variability from cell cultures for plant improvement

“…This demands an efficient and sustained capacity to regenerate plants from cell cultures. Recent research has demonstrated that such a requirement is realisable in previously recalcitrant crop plants such as wheat (Shimada and Yamada 1979), rice (Oono 1978b) and important legumes (Bingham et al 1975;Beach and Smith 1979;Phillips and Collins 1979;Kao and Michayluk 1980;dos Santos et al 1980) possibly including soybean (Saka et al 1980). Somaclonal variation may find its greatest application for plant improvement in concert with selection for desirable mutations at the cellular level.…”

Plant regeneration from cotyledonary tissue of Glycine canescens, a perennial wild relative of soybean

“…In conditioned cotyledonary node segments of G. max (soybean) [5] a high cytokinin concentration stimulated shoot-bud growth but inhibited further growth of the bud. In the present study, growth proceeded almost normally in the presence of high cytokinin although in some cases apical dominance was suppressed and multiple shoots arose.…”

Differences in the Rates of Ethylene Production and Growth between the Calluses Derived from Rice (Oryza sativaL.) and Soybean (Glycine max(L.) Merr.)

“…Gaspar et al (1989) reported that IBA and BAP given at same concentration showed a synergistic effect on the production of ethylene in rose explants. Saka et al (1980) also reported that soybean callus formation, growth and regeneration were dependent on the combination of IBA and BAP. in the medium.…”