Stimulation of mesothelial cell replication in vivo by a macrophage-derived factor

Mutsaers, Steve; Whitaker, Darrel; Papadimitriou, J. M.

doi:10.1016/s0954-6111(05)80223-9

Cited by 1 publication

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“…While the role of FGF2 and PDGF in adult mesothelial cell proliferation is known, their impact during development is little known 14 . To confirm each molecule’s effect on developing MPCs, we cultured MPC with FGF2 and PDGF-BB for 3 days ( Figure 2 ).…”

Section: Resultsmentioning

confidence: 99%

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FGF2 promotes the expansion of parietal mesothelial progenitor pools and inhibits BMP4-mediated smooth muscle cell differentiation

Hwang,

Shimamura,

Tanaka

et al. 2024

Preprint

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SummaryMesothelial cells, in the outermost layer of internal organs, are essential for both organ development and homeostasis. Although the parietal mesothelial cell is the primary origin of mesothelioma that may highjack developmental signaling, the signaling pathways that orchestrate developing parietal mesothelial progenitor cell (MPC) behaviors, such as MPC pool expansion, maturation, and differentiation, are poorly understood. To address it, we established a robust protocol for culturing WT1+MPCs isolated from developing pig and mouse parietal thorax. Quantitative qPCR and immunostaining analyses revealed that BMP4 facilitated MPC differentiation into smooth muscle cells (SMCs). In contrast, FGF2 significantly promoted MPC progenitor pool expansion but blocked the SMC differentiation. BMP4 and FGF2 counterbalanced these effects, but FGF2 had the dominant impact in the long-term culture. A Wnt activator, CHIR99021, was pivotal in MPC maturation to CALB2+mesothelial cells, while BMP4 or FGF2 was limited. Our results demonstrated central pathways critical for mesothelial cell behaviors.

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Section: Resultsmentioning

confidence: 99%

“…Previous in vitro studies have shown that Fibroblast growth factor 2 (FGF2) and platelet-derived growth factor (PDGF) are required for the proliferation of adult mesothelial cells 14 . Notably, high expression of FGF2 in mesothelioma correlated with poor prognosis 15 .…”

Section: Introductionmentioning

confidence: 99%