Stimulation of long-term ammonium nitrogen deposition on methanogenesis by Methanocellaceae in a coastal wetland

Xiao, Leilei; Xie, Baohua; Liu, Jinchao; Zhang, Hongxia; Han, Guoqi; Wang, Oumei; Liu, Fanghua

doi:10.1016/j.scitotenv.2017.03.279

Cited by 44 publications

(26 citation statements)

References 40 publications

(37 reference statements)

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“…It should be noted that this response of N 2 O emission pattern to the frequency of N addition did not affect the CO 2 eq estimates, because N 2 O emission was low, although the warming effect of N 2 O is 298 times higher than that of CO 2 [3]. CH 4 emission is related to the methanogens and methanotrophs in the soil [4,43]. N addition can increase the availability of soil N, thereby altering the microbial processes of CH 4 production and oxidation [44,45].…”

Section: Effects Of the Quantity And Frequency Of N Addition On Greenmentioning

confidence: 84%

“…In recent years, more attention has been paid to the increasing atmospheric nitrogen (N) deposition caused by human activities [1]. N deposition has continued to increase over the past 50 years [2], influencing the structure and function of various ecosystems [3][4][5]. At present, N deposition flux in China is about 2.1 g N m −2 yr −1 , and that in Zhejiang Province is about 5 g N m −2 yr −1 [6].…”

Section: Introductionmentioning

confidence: 99%

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Effects of N Addition Frequency and Quantity on Hydrocotyle vulgaris Growth and Greenhouse Gas Emissions from Wetland Microcosms

Zhang

Gao

et al. 2019

Sustainability

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(1) Background: Increased attention has been paid to atmospheric nitrogen (N) deposition caused by human activities. N deposition quantity has seriously affected plant productivity and greenhouse gas emissions in wetlands, but the effects of N deposition frequency remain unclear. (2) Methods: We assembled microcosms, which contained vegetative individuals (ramets) of Hydrocotyle vulgaris and soil and subjected them to three frequencies (N addition 1, 2, and 14 times during the experimental period) crossed with three quantities (5, 15, and 30 g N m−2 yr−1) for 90 days. (3) Results: The quantity of N addition significantly increased the root, stem biomass, and ramets number of H. vulgaris, but decreased the spike biomass. N addition quantity significantly promoted N2O emission and inhibited CH4 emission but had no significant effect on CO2 emission. The increasing frequency of N addition significantly promoted the root-to-shoot ratio and decreased N2O emission under high N addition quantity. (4) Conclusions: In conclusion, N addition alters the reproductive strategy of H. vulgaris and enhances its invasiveness, promoting N2O emission but not the CO2 equivalent of the H. vulgaris-soil system.

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Section: Effects Of the Quantity And Frequency Of N Addition On Greenmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Effects of N Addition Frequency and Quantity on Hydrocotyle vulgaris Growth and Greenhouse Gas Emissions from Wetland Microcosms

Zhang

Gao

et al. 2019

Sustainability

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“…Seasonal variations in climate may have a considerable effect on methanogenesis in wetland ecosystems and is also an underlying driving factor affecting the structure of methanogenic communities [32,43,44]. Methanogens are sensitive to seasonal variation due to changes in oxygen availability and temperature [45], and thus the abundance and composition of methanogens is very variable in wetland ecosystems.…”

Section: Discussionmentioning

confidence: 99%

Characteristics and Metabolic Patterns of Soil Methanogenic Archaea Communities in the High Latitude Natural Wetlands of China

Zhao

Bai

et al. 2020

Preprint

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Background: Soil methanogenic microorganisms are one of the primary methane-producing microbes in wetlands. However, we still poorly understand the community characteristic and metabolic patterns of these microorganisms according to vegetation type and seasonal changes. Therefore, to better elucidate the effects of the vegetation type and seasonal factors on the methanogenic community structure and metabolic patterns, we detected the characteristics of the soil methanogenic mcrA gene from three types of natural wetlands in different seasons in the Xiaoxing'an Mountain region, China. Result: The results indicated that the distribution of Methanobacteriaceae (hydrogenotrophic methanogens) was higher in winter, while Methanosarcinaceae and Methanosaetaceae accounted for a higher proportion in summer. Hydrogenotrophic methanogenesis was the dominant trophic pattern in each wetland. The results of principal coordinate analysis and cluster analysis showed that the vegetation type considerably influenced the methanogenic community composition. The methanogenic community structure in the Betula platyphylla – Larix gmelinii wetland was relatively different from the structure of the other two wetland types. Indicator species analysis further demonstrated that the corresponding species of indicator operational taxonomic units from the Alnus sibirica wetland and the Betula ovalifolia wetland were closer. Network analysis showed that cooperative and competitive relationships exist both within and between the same or different trophic methanogens. The core methanogens with higher abundance in each wetland were conducive to adaptation to environmental disturbances. Conclusions: This information is crucial for the assessment of metabolic patterns of soil methanogenic archaea and future fluxes in the wetlands of the Xiaoxing'an Mountain region given their vulnerability.

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“…Despite this, no methanogenic activity was detected in our incubations under any of the experimental conditions tested. Archaeal members of the RC-I cluster have previously been detected in wetland and marsh ecosystems (Lin et al, 2017;Xiao et al, 2017), as well as in rice paddies. These organotrophic systems contain high amounts of endlessly decomposing NOM, which prevail under anoxic conditions due to flooding, thus creating the proper niche for microbes to perform the redox reactions involved in AOM coupled to N 2 O reduction, mediated by humic substances (Figure 5).…”

Section: Microbial Communities Potentially Involvedmentioning

confidence: 97%

Humic Substances Mediate Anaerobic Methane Oxidation Linked to Nitrous Oxide Reduction in Wetland Sediments

et al. 2020

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Humic substances are redox-active organic molecules, which play pivotal roles in several biogeochemical cycles due to their electron-transferring capacity involving multiple abiotic and microbial transformations. Based on the redox properties of humic substances, and the metabolic capabilities of microorganisms to reduce and oxidize them, we hypothesized that they could mediate the anaerobic oxidation of methane (AOM) coupled to the reduction of nitrous oxide (N 2 O) in wetland sediments. This study provides several lines of evidence indicating the coupling between AOM and the reduction of N 2 O through an extracellular electron transfer mechanism mediated by the redox active functional groups in humic substances (e.g., quinones). We found that the microbiota of a sediment collected from the Sisal wetland (Yucatán Peninsula, southeastern Mexico) was able to reduce N 2 O (4.6 ± 0.5 µmol N 2 O g sed. −1 day −1) when reduced humic substances were provided as electron donor in a close stoichiometric relationship. Furthermore, a microbial enrichment derived from the wetland sediment achieved simultaneous 13 CH 4 oxidation (1.3 ± 0.1 µmol 13 CO 2 g sed. −1 day −1) and N 2 O reduction (25.2 ± 0.5 µmol N 2 O g sed. −1 day −1), which was significantly dependent on the presence of humic substances as an extracellular electron shuttle. Taxonomic characterization based on 16S rRNA gene sequencing revealed Acinetobacter (a G-proteobacterium), the Rice Cluster I from the Methanocellaceae and an uncultured archaeon from the Methanomicrobiaceae family as the microbes potentially involved in AOM linked to N 2 O reduction mediated by humic substances. The findings reported here suggest that humic substances might play an important role to prevent the emission of greenhouse gases (CH 4 and N 2 O) from wetland sediments. Further efforts to evaluate the feasibility of this novel mechanism under the natural conditions prevailing in ecosystems must be considered in future studies.

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Stimulation of long-term ammonium nitrogen deposition on methanogenesis by Methanocellaceae in a coastal wetland

Cited by 44 publications

References 40 publications

Effects of N Addition Frequency and Quantity on Hydrocotyle vulgaris Growth and Greenhouse Gas Emissions from Wetland Microcosms

Effects of N Addition Frequency and Quantity on Hydrocotyle vulgaris Growth and Greenhouse Gas Emissions from Wetland Microcosms

Characteristics and Metabolic Patterns of Soil Methanogenic Archaea Communities in the High Latitude Natural Wetlands of China

Humic Substances Mediate Anaerobic Methane Oxidation Linked to Nitrous Oxide Reduction in Wetland Sediments

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