Stimulation of intestinal Cl- transport by heat-stable enterotoxin: activation of cAMP-dependent protein kinase by cGMP

Forte, Leonard R.; Thorne, Pamela K.; Eber, Sammy L.; Krause, William J.; Freeman, Ronald H.; Francis, Sharron H.; Corbin, Jackie D.

doi:10.1152/ajpcell.1992.263.3.c607

Cited by 245 publications

(86 citation statements)

References 8 publications

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“…Most actions of cyclic GMP on vascular cells are due to the activation of its major intracellular target cyclic GMP-dependent protein kinase (Lincoln & Cornwell, 1993). Cyclic GMP may also affect vascular functions via interaction with the cyclic AMP signalling pathway, due to its ability to activate protein kinase(s) A at high concentrations which is closely related in structure and function to the cyclic GMP-dependent protein kinase (Forte et al, 1992;Cornwell et al, 1994a). Moreover cyclic GMP inhibits the activity of the vascular smooth muscle phosphodiesterase III which preferentially hydrolyzes cyclic AMP (Beavo & Reifsnyder, 1990).…”

Section: Expression Of Inos Proteinmentioning

confidence: 99%

Effect of cyclic GMP‐dependent vasodilators on the expression of inducible nitric oxide synthase in vascular smooth muscle cells: role of cyclic AMP

Boese

Busse

Mülsch

et al. 1996

British J Pharmacology

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1 In the present study we examined whether interleukin-lf (IL-Ifl) increases the activity of adenylyl cyclase in vascular smooth muscle cells and determined its role in the cytokine-induced expression of the inducible nitric oxide synthase (iNOS) and activation of nuclear transcription factor-KB (NF-KB). In addition the interaction between cyclic AMP-and cyclic GMP-elevating agonists on the IL-iIf-stimulated expression of iNOS was examined.2 Exposure of vascular smooth muscle cells to IL-,IB stimulated the formation of cyclic AMP but not of cyclic GMP. The intracellular level of cyclic AMP reached a maximum within 1 h and then gradually declined over the next 5 h. This IL-l, (60 u ml-')-stimulated formation of cyclic AMP was modest (about 3 fold at 60 u ml-' for 1 h) compared to that evoked by isoprenaline (about 9 fold at 3 x 10-6 M for 2 min). 3 The IL-iI (60 u ml1 for 24 h)-stimulated accumulation of nitrite, which was taken as an index of NO production, was concentration-dependently increased by preferential inhibitors of cyclic AMP-dependent phosphodiesterases (rolipram and trequinsin). This effect was reproduced by a specific activator of the cyclic AMP-dependent protein kinase(s) A, Sp-8-CPT-cAMPS (10-4 M) but was prevented by a specific inhibitor of cyclic AMP-dependent protein kinase(s) A, Rp-8-CPT-cAMPS (10-4 M). These compounds alone [rolipram (10-6 M), trequinsin (3 x 10-6 M) and Sp-8-CPT-cAMPS (l0-4 M)] slightly but significantly increased the release of nitric oxide while Rp-8-CPT-cAMPS elicited no such effect. 4 Inducible NOS protein was expressed in IL-/IB (30 u ml-', 24 h)-stimulated smooth muscle cells as assessed by Western blot analysis. The level of iNOS protein was markedly increased in smooth muscle cells which had been exposed to IL-,IB in combination with either rolipram (3 x 10-6 M) or Sp-8-CPTcAMPS (l0-4 M) but was reduced in those exposed to IL-,IB and Rp-8-CPT-cAMPS (l0-4 M). A weak expression of iNOS protein was found in smooth muscle cells which had been exposed to either Sp-8-CPT-cAMPS or rolipram alone for 24 h while Rp-8-CPT-cAMPS elicited no such effect. 5 Exposure of smooth muscle cells to IL-lI (30 u ml-') for 30 min increased the level of NF-KB-DNA complexes in nuclear extracts as detected by electrophoretic mobility shift assay. Similar levels of NF-KB-DNA complexes were found in cells which had been exposed to IL-,IB in combination with either Sp

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Section: Expression Of Inos Proteinmentioning

confidence: 99%

Effect of cyclic GMP‐dependent vasodilators on the expression of inducible nitric oxide synthase in vascular smooth muscle cells: role of cyclic AMP

Boese

Busse

Mülsch

et al. 1996

British J Pharmacology

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“…It leads to hypokalemia by inducing potassium secretion in the intestines. It may cause to hypochlorhydria with inhibition of gastric acid secretion (4,5). In addition to these effects, it may cause to hypercalcemia by increasing bone resorption, flushing on the face by dilating blood vessels and hyperglycemia by way of hepatic glycogenolysis (2).…”

Section: Discussionmentioning

confidence: 99%

Vasoactive intestinal peptide releasing tumor which caused to chronic watery diarrhea and hypokalemia

Kanık¹,

Baran²,

Çayan³

et al. 2014

Turk Arch Ped

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IntroductionWatery diarrhea, hypokalemia and achlorhydria syndrome which was described by Verner and Morrison (1) in 1958 for the first time (WDHA) is a rare cause of chronic secretory diarrhea arising from a vasoactive intestinal peptide (VIP) secreting tumor. In adults, the majority of WDHA syndrome arises from pancreatic islet cell adenoma and hyperplasia (2, 3). In contrast, it mostly arises from VIP secreting tumor originating from the neural crest cells in the adrenal medulla or sympathetic ganglia in children (4). In this article, a 15-month old female patient who could not be diagnosed despite investigations made in different centers because of watery diarrhea, severe malnutrition and abdominal distention which had been lasting for four months and who was later diagnosed with VIP secreting gangliobeuroblastoma localized in the right side was presented. CaseA 15-month old female baby presented to our hospital because of bulky watery diarrhea approximately 10-12 times a day which had been lasting for four months. She was investigated in two centers before because of chronic diarrhea and no response was obtained with the treatments administered. The patient who had a birth weight and height at the 50 th percentile had a weight of 7 080 g (-6,1 SD) and a height of of 64 cm (-4,9 SD) at presentation. Her vital findings were within the normal limits. She looked weak and cachectic and had abdominal distension and increased pigmentation on the skin. Other physical examination findings were found to be normal (Figure 1). Laboratory tests were as follows: hemoglobin 9.6 g/dL, WBC 16 100/mm³, platelets 611 000/mm³, serum sodium 130 mmol/L, potassium 2.3 mmol/L, Chlorine 104 mmol/L. Blood gas analysis revealed metabolic acidosis. Stool osmotic gap was found to be 32 mosm/L (secretory diarrhea) and reducing substance and fat were found to be negative in stool. Stool culture and parasite examination were found to be negative. Serum glucose, renal and hepatic function AbstractWatery diarrhea, hypokalemia and achlorhydria syndrome is a rare cause of chronic secretory diarrhea arising from a vasoactive intestinal peptide releasing tumor. In this article, a 15-month old female patient with watery diarrhea and abdominal distension which lasting four months is presented. In different centers no diagnosis could be made although investigations. The patient was diagnosed with vasoactive intestinal peptide releasing ganglioneuroblastoma localized in the right surrenal gland. (Türk Ped Arş 2014; 49: 160-2)

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“…Although there are no reports of WT1 expression in lung epithelium, both kidney epithelial and breast epithelial cells have been reported to express this factor (24). Interestingly, WT1 is regulated by protein kinase A (PKA) (24 -26), a downstream mediator of sGC (27)(28)(29). PKA-mediated phosphorylation of WT1 induces its translocation from the nucleus to the cytosol.…”

Section: Atrix Metalloproteinases (Mmp) 2 Degrade Collagensmentioning

confidence: 99%

“…Active sGC produces cGMP and the latter can mediate gene regulation through the activation of cGMP-dependent protein kinase (PKG) and cAMP-dependent protein kinase (PKA) (27)(28)(29). To test the role of PKG and PKA in the signal transduction of this pathway, we used the membrane soluble PKG-and PKA-specific inhibitors AD-2 and myrPKA, respectively.…”

Section: Pka and Sgc Are Involved In Mmp-9 Gene Regulationmentioning

confidence: 99%

The Transcription Factor Wilms Tumor 1 Regulates Matrix Metalloproteinase-9 through a Nitric Oxide-Mediated Pathway

Marcet‐Palacios

Ulanova

Duta

et al. 2007

The Journal of Immunology

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Matrix metalloproteinase-9 (MMP-9) is released by human lung epithelial cells (LEC) in conditions such as asthma and chronic obstructive pulmonary disease and expression of MMP-9 correlates with the severity of these disorders. MMP-9 production has been reported to be regulated by a NO/soluble guanylate cyclase-dependent pathway. Transcriptional regulation of this enzyme, however, is poorly understood. Using phylogenetic analysis, we observed a highly conserved sequence in the 5′ flanking region of the MMP-9 gene containing binding sites for the transcription factor Wilms tumor 1 (WT1). We confirmed the presence of WT1 in human LEC and that treatment with TNF or a mixture containing LPS, PMA, and IFN-γ resulted in translocation of WT1 from the nucleus to the cytosol. This translocation coincided with increased expression of MMP-9 and could be blocked by inhibitors of the NO/soluble guanylate cyclase pathway. WT1 knockdown using small-interfering RNA up-regulated MMP-9 expression in the presence of the NO synthase inhibitor 1400W. Using either WT1 pulldown with probes for the conserved region of the MMP-9 promoter or chromatin immunoprecipitation, we confirmed WT1 binding to the MMP-9 promoter. These findings indicate WT1 is a repressor of MMP-9, regulated by a NO-mediated pathway in human LEC. To our knowledge, this is the first report of WT1 regulating MMP-9 expression. Further study is needed to determine whether clinical conditions exhibiting tissue remodeling, such as asthma and/or chronic obstructive pulmonary disease, demonstrate reduced levels of WT1 or its repressor activity.

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Stimulation of intestinal Cl- transport by heat-stable enterotoxin: activation of cAMP-dependent protein kinase by cGMP

Cited by 245 publications

References 8 publications

Effect of cyclic GMP‐dependent vasodilators on the expression of inducible nitric oxide synthase in vascular smooth muscle cells: role of cyclic AMP

Effect of cyclic GMP‐dependent vasodilators on the expression of inducible nitric oxide synthase in vascular smooth muscle cells: role of cyclic AMP

Vasoactive intestinal peptide releasing tumor which caused to chronic watery diarrhea and hypokalemia

The Transcription Factor Wilms Tumor 1 Regulates Matrix Metalloproteinase-9 through a Nitric Oxide-Mediated Pathway

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