Stimulation of in Vitro Bulblet Growth in Galanthus Species With Sucrose and Activated Charcoal

Staikidou, I.; Selby, Christopher P.; Hanks, Gordon R.

doi:10.17660/actahortic.2006.725.57

Cited by 6 publications

(6 citation statements)

References 12 publications

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“…Similar to what we have observed, Staikkidou et al (2000) stated that charcoal effected the development of the plantlets by inducing the formation of large number of roots and stimulating root elongation.…”

Section: Resultssupporting

confidence: 90%

“…Under in vitro culture conditions, the growth of the bulblets of F. imperialis can be stimulated by a combination of plant growth hormones (Li et al, 2011). Suitable bulblet size for acclimatization is also achieved by transferring the plantlets to a growth medium containing no plant growth regulator (PGR) but an elevated level of carbohydrate contained with activated carbon (Staikkidou et al, 2000).…”

Section: -Benzilaminopurin Ve Aktif Karbonun Fritillaria Imperialis'in Dolaylı Organogenezi üZerindeki Etkilerimentioning

confidence: 99%

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Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis

Saeed

Cömertpay

2020

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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Fritillaria imperialis needs 2-3 years to regenerate from seeds, which makes its production quite challenging. In this study, effects of three concentrations of 6-Benzylaminopurine (BAP) (0.00, 0.10 and 0.15 mg/L) and three different amounts (0.0, 3.0 and 3.5 g/L) of activated carbon (AC) on the growth of F. imperialis plantlets in vitro were determined. The auxins were at similar concentration (0.01 mg/L of NAA and 0.01 mg/L of IAA) for all treatments. It has been found that the size and number of bulblets, as well as root and shoot numbers in regenerated plantlets were increased significantly (p<0.05) by adding 3.5 g/L of AC in culture medium supplemented with 0.10 mg/L of BAP. Moreover, addition of AC significantly reduced the length of roots in all doses studied (p<0.05). The supreme concentration of BAP (0.15 mg/L) decreased the bulblet number in all treatments, however, when supplemented with 3.5 g/L of AC, it was found to raise all the values measured (p<0.05). Overall, the best growth of the plant parts from callus were achieved with the highest concentration of AC and 1.5 mg/L of BAP.

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Section: Resultssupporting

confidence: 90%

Section: -Benzilaminopurin Ve Aktif Karbonun Fritillaria Imperialis'in Dolaylı Organogenezi üZerindeki Etkilerimentioning

confidence: 99%

Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis

Saeed

Cömertpay

2020

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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“…Aasim et al (2011) reported a low rooting frequency at 3.0% sucrose in chickpea. Those results clearly showed the influence of sucrose concentration on rooting, in agreement with Staikidou et al (2006), who also used increased sucrose concentrations for rooting in Galanthus species.…”

Section: Discussionsupporting

confidence: 89%

Multiple shoot regeneration of plumular apices of chickpea

Glinska¹,

Jankowski²,

Kubiak³

et al. 2013

Turkish Journal of Agriculture and Forestry

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“…Activated charcoal adsorbs inhibitory substances in the culture medium (Fridborg et al 1978;Weatherhead et al 1979) and thus is often used to reduce phenolic compounds in tissue culture to improve growth efficiency (Carlberg et al 1983;Teixeria et al 1994). The stimulatory role of activated charcoal for plant regeneration has been reported from various tissue culture systems, including protoplastderived callus (Guo et al 2007;Kunitake et al 1995), bulblet growth (Staikidou et al 2006), embryo maturation and conversion (Kamo et al 2004), haploid plant production (Gürel et al 2000), sporophyte regeneration (Teng 1997), rooting (Dumas and Monteuuis 1995), and shoot regeneration (Nhut et al 2001).…”

Section: Effects Of Activated Charcoal and Zeatin On Plantlet Developmentioning

confidence: 99%

High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)

Choi

et al. 2008

Plant Biotechnol Rep

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An improved protocol for high frequency plant regeneration via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield (Brasenia schreberi) was developed. Zygotic embryos formed pale-yellow globular structures and white friable callus at a frequency of 80% when cultured on halfstrength MS medium supplemented with 0.3 mg l -1 2,4-D. However, the frequency of formation of pale-yellow globular structures and white friable callus decreased slightly with increasing concentrations of 2,4-D up to 3 mg l -1 , where the frequency reached *50% of the control. Cell suspension cultures from zygotic embryoderived white friable callus were established using halfstrength MS medium supplemented with 0.3 mg l -1 2,4-D. Upon plating of cell aggregates on half-strength MS basal medium, approximately 8.3% gave rise to somatic embryos and developed into plantlets. However, the frequency of plantlet development from cell aggregates was sharply increased (by up to 55%) when activated charcoal and zeatin were applied. Regenerated plantlets were successfully transplanted to potting soil and grown to normal plants in a growth chamber. The distinctive feature of this study is the establishment of a high frequency plant regeneration system via somatic embryogenesis from zygotic embryo-derived cell suspension cultures of watershield, which has not been previously reported. The protocol for plant regeneration of watershield through somatic embryogenesis could be useful for the mass propagation and transformation of selected elite lines.

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Stimulation of in Vitro Bulblet Growth in Galanthus Species With Sucrose and Activated Charcoal

Cited by 6 publications

References 12 publications

Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis

Effects of 6-benzylaminopurine and Activated Carbon on Indirect Organogenesis of Fritillaria Imperialis

Multiple shoot regeneration of plumular apices of chickpea

High frequency plant regeneration from zygotic-embryo-derived embryogenic cell suspension cultures of watershield (Brasenia schreberi)

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