Stimulation of humoral antibody formation by polyanions. III. Restoration of the immune response to sheep red blood cells by polyanions in thymectomized and lethally irradiated mice protected with bone marrow cells

Diamantstein, T; Wagner, Barbara; L’age-Stehr, Johanna; Beyse, Inge; Odenwald, Maria V.; Schultz, Gisela

doi:10.1002/eji.1830010418

Cited by 40 publications

(12 citation statements)

References 7 publications

(1 reference statement)

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“…A similar conclusion was reached by investigators studying the replacement of T cell function by polyanions (7,14,15). The release of a soluble mediator into culture supernatant from activated thymus-derived (16)(17)(18) or thymus cells (19,20) was shown by its effect on other cultured cells.…”

Section: Methodssupporting

confidence: 59%

Thymus-Derived Cell (T Cell) Activation by Heterologous Antigens as a Replacement of Specific Immune T Cells in the Transfer of the Secondary Response to Sheep Erythrocytes

Mond

Takahashi

Thorbecke

1972

The Journal of Experimental Medicine

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Spleen cells from LAF1 mice hyperimmune to sheep erythrocytes (SE) lost their ability to transfer a secondary response to irradiated recipients after incubation with anti-θ and rabbit complement in vitro. Small numbers of specific immune cells even when taken 3 days after a primary SE injection reconstituted the direct and indirect plaque-forming cell responses. Larger numbers of cells sensitized to B. abortus (or keyhole limpet hemocyanin), and given together with the corresponding antigen, also partially reconstituted the ability to respond to SE. This property was mediated by θ-bearing cells and was interpreted as due to a nonspecific humoral factor liberated by specifically activated T cells and acting on B cell proliferation or maturation.

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Section: Methodssupporting

confidence: 59%

Thymus-Derived Cell (T Cell) Activation by Heterologous Antigens as a Replacement of Specific Immune T Cells in the Transfer of the Secondary Response to Sheep Erythrocytes

Mond

Takahashi

Thorbecke

1972

The Journal of Experimental Medicine

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“…Polyanionic substances possess adjuvant activities for cell-mediated and humoral immune responses to different types of antigens [7,11,19,25,33]. The mechanism behind these adjuvant effects was sug gested to be interference with phagocyte functions [10,16] which would be in favour of the persistence of the antigen [7,27,38,39], This explanation is in line with observations made in Biozzi mice, suggesting that an impaired phagocyte function was related to high immune-responsiveness and vice versa [4,37], Polyanions have also effects on the complement (C) cascade.…”

Section: Introductionmentioning

confidence: 99%

Analytic Study of the Differential Anticomplementary Effects of Dextran Sulphate and Heparin in the Assay for the Mouse Alternative Pathway

Klerx¹,

Dijk²,

Maaden³

et al. 1985

Int Arch Allergy Immunol

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In a recent paper, a linkage between immunological adjuvant activity in mice and in vitro anticomplementary (alternative pathway assay) effects was described for different polyanions. This connection was found only if mouse serum was used as a complement (C) source. In order to investigate the possible role of C in adjuvant activity, the differential effects of polyanions on mouse C were studied in detail. For this study, substances with different activity were selected, namely dextran sulphate with strong C-regulatory and immunoadjuvant activities, and heparin, which was weakly anticomplementary and devoid of adjuvant effect. In general, studies of mouse C are complicated by the inavailability of isolation procedures for the C-components involved. This difficulty was circumvented by making use of C5-deficient serum and of the haemolytic activity of mouse membrane attack complexes formed in the fluid phase. With yeast cells as alternative pathway activators it was shown that the effect of heparin on this pathway was restricted to activation of the terminal route. In contrast, dextran sulphate also caused a functional decay of a yeast-bound alternative pathway C5-convertase and interfered with the haemolytic activity of fluid-phase membrane attack complexes as well. Further studies will be needed to decide whether these specific effects of dextran sulphate are related to the immunological adjuvant activity of the substance in mice.

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“…On the other hand, humoral factors extracted from thymus [ 13-171 or from thymus cells [ 1.81 or recently found in fetal serum [19], which probably d o not carry a specific message, have b2en considered t o be involved in the T and B cell interaction. Recently, we have reported that polyanions are capable of replacing T cell function in bone marrow-protected, thymectomized and lethally irradiated mice [20]. Furthermore, we would like t o consider that perhaps an artificial effect protects such immunologically unrelated cells to concentrate and t o present an antigen for t h e B cell.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of humoral antibody formation by polyanions. V. Relationship between enhancement of sheep red blood cell uptake by the spleen and adjuvant action of dextran sulfate

1971

Self Cite

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The effect of a polyanion, dextran sulfate, on the uptake of 51Cr‐labeled sheep red blood cells (51Cr‐SRBC) by the spleen of mice was investigated. Enhancement of 51Cr‐SRBC uptake by the spleen could only be detected under the conditions which had previously been detected as requirement for the adjuvant action of dextran sulfate. Plaque‐forming cell (PFC) values assayed in the spleen of mice injected with dextran sulfate and graded amounts of SRBC were compared with PFC values of controls and PFC values calculated from effective antigen amounts, i. e. total radioactivity measured in the spleen of dextran sulfate‐treated animals. PFC values assayed were found to correlate with the PFC values calculated. The possible significance of increased antigen uptake by the spleen for the mechanism of adjuvant action of polyanions has been discussed.

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Stimulation of humoral antibody formation by polyanions. III. Restoration of the immune response to sheep red blood cells by polyanions in thymectomized and lethally irradiated mice protected with bone marrow cells

Cited by 40 publications

References 7 publications

Thymus-Derived Cell (T Cell) Activation by Heterologous Antigens as a Replacement of Specific Immune T Cells in the Transfer of the Secondary Response to Sheep Erythrocytes

Thymus-Derived Cell (T Cell) Activation by Heterologous Antigens as a Replacement of Specific Immune T Cells in the Transfer of the Secondary Response to Sheep Erythrocytes

Analytic Study of the Differential Anticomplementary Effects of Dextran Sulphate and Heparin in the Assay for the Mouse Alternative Pathway

Stimulation of humoral antibody formation by polyanions. V. Relationship between enhancement of sheep red blood cell uptake by the spleen and adjuvant action of dextran sulfate

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