Stimulation of Human Anti-Viral CD8+ Cytolytic T Lymphocytes by Dendritic Cells

Bhardwaj, Nina; Bender, Armin; González, Noemí; Bui, Long Kim; Garrett, Maria Carolina; Steinman, Ralph M.

doi:10.1007/978-1-4615-1971-3_84

Cited by 17 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PD-L2 ͉ IgM ͉ synergy ͉ cytotoxic T cells ͉ costimulation D endritic cells (DCs) are heterogenous populations of cells that play important roles in initiating immune responses by their ability to actively acquire antigen and process and present peptides in the context of MHC class I and II molecules to naïve CD8 and CD4 T cells (1)(2)(3)(4). Mature DCs (mDCs) express an array of costimulatory molecules, including B7.1, B7.2, CD40, and the recently identified B7-DC͞PD-L2.…”

Section: B7-dc Cross-linking Restores Antigen Uptake and Augments Antmentioning

confidence: 99%

RETRACTED: B7-DC cross-linking restores antigen uptake and augments antigen-presenting cell function by matured dendritic cells

Radhakrishnan

Celis

Pease³

2005

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Dendritic cells (DCs) are classified in two states: immature DCs (iDCs), which perform sentinel functions, sampling for antigen and danger signals, and mature DCs (mDCs), which exhibit enhanced antigen-presenting functions but are no longer capable of acquiring antigen. We now describe DCs with a different activation phenotype: cells having the strong antigen-presenting functions of mDCs and the antigen-acquiring functions of iDCs. We have described an antibody that binds the costimulatory molecule B7-DC and activates DCs. The resulting phenotype is distinct from iDCs or mDCs matured by using Toll-like receptor (TLR) agonists. Ability to take up antigen increases, while expression of B71/2 costimulatory and MHC molecules remains unchanged. DCs matured with TLR agonists and then superactivated through B7-DC exhibit a previously unrecognized phenotype. These DCs recover the ability to take up antigen, which is normally lost after treatment with TLR-3 and TLR-9 agonists, yet retain the high expression of costimulatory and MHC molecules and strong antigen-presenting functions of mDCs. Immunization using TLR agonists and B7-DC XAb (cross-linking antibody) together as adjuvant resulted in substantially increased cytolytic T cell responses, particularly when minimal peptide antigens were used. By stimulating DCs with two distinct activation signals, a previously unrecognized phenotype exhibiting augmented antigen-presenting functions was obtained

show abstract

Section: B7-dc Cross-linking Restores Antigen Uptake and Augments Antmentioning

confidence: 99%

RETRACTED: B7-DC cross-linking restores antigen uptake and augments antigen-presenting cell function by matured dendritic cells

Radhakrishnan

Celis

Pease³

2005

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Movement of antigens to the lymphoid organs may be critical for the induction of T-cell immunity (19,48). DC have the unique capacity to present antigens in the lymphoid organs and induce antigen-specific CTL activity by priming naïve CD4 ϩ and CD8 ϩ lymphocytes (3,7,18,24). Our challenge was to express the proteins encoded by the retrovirus vector in DC.…”

Section: Resultsmentioning

confidence: 99%

Induction of Potent Human Immunodeficiency Virus Type 1-Specific T-Cell-Restricted Immunity by Genetically Modified Dendritic Cells

Lisziewicz

Gabrilovich

Varga

et al. 2001

J Virol

View full text Add to dashboard Cite

A novel technology combining replication-and integration-defective human immunodeficiency virus type 1 (HIV-1) vectors with genetically modified dendritic cells was developed in order to induce T-cell immunity. We introduced the vector into dendritic cells as a plasmid DNA using polyethylenimine as the gene delivery system, thereby circumventing the problem of obtaining viral vector expression in the absence of integration. Genetically modified dendritic cells (GMDC) presented viral epitopes efficiently, secreted interleukin 12, and primed both CD4؉ and CD8 ؉ HIV-specific T cells capable of producing gamma interferon and exerting potent HIV-1-specific cytotoxicity in vitro. In nonhuman primates, subcutaneously injected GMDC migrated into the draining lymph node at an unprecedentedly high rate and expressed the plasmid DNA. The animals presented a vigorous HIV-specific effector cytotoxic-T-lymphocyte (CTL) response as early as 3 weeks after a single immunization, which later developed into a memory CTL response. Interestingly, antibodies did not accompany these CTL responses, indicating that GMDC can induce a pure Th1 type of immune response. Successful induction of a broad and long-lasting HIV-specific cellular immunity is expected to control virus replication in infected individuals.

show abstract

“…Therefore, early studies were focused on identifying the primary cell population involved in initiating virus-specific Tcell immunity following IAV infection. In vitro exposure of DC to IAV was shown to be sufficient to stimulate a primary virusspecific CD8 T-cell response from LN-purified T cells [73][74][75][76]. Further, IAV-infected DC were particularly potent inducers of IAV-specific immunity relative to other APC subsets and could trigger significant CD8 T-cell responses without the addition of exogenous cytokines [73,75].…”

Section: Activation Following Iav Infectionmentioning

confidence: 96%

Innate immune control and regulation of influenza virus infections

McGill

Heusel

Legge

2009

Journal of Leukocyte Biology

145

150

View full text Add to dashboard Cite

Adaptive immune responses are critical for the control and clearance of influenza A virus (IAV) infection. However, in recent years, it has become increasingly apparent that innate immune cells, including natural killer cells, alveolar macrophages (aMphi), and dendritic cells (DC) are essential following IAV infection in the direct control of viral replication or in the induction and regulation of virus-specific adaptive immune responses. This review will discuss the role of these innate immune cells following IAV infection, with a particular focus on DC and their ability to induce and regulate the adaptive IAV-specific immune response.

show abstract

Stimulation of Human Anti-Viral CD8+ Cytolytic T Lymphocytes by Dendritic Cells

Cited by 17 publications

References 34 publications

RETRACTED: B7-DC cross-linking restores antigen uptake and augments antigen-presenting cell function by matured dendritic cells

RETRACTED: B7-DC cross-linking restores antigen uptake and augments antigen-presenting cell function by matured dendritic cells

Induction of Potent Human Immunodeficiency Virus Type 1-Specific T-Cell-Restricted Immunity by Genetically Modified Dendritic Cells

Innate immune control and regulation of influenza virus infections

Contact Info

Product

Resources

About