Stimulation of H<sup>+</sup>extrusion and plasma membrane H<sup>+</sup>-ATPase activity of barley roots by ammonium-treatment

Yamashita, Kanae; Kasai, Minobu; Ezaki, Bunichi; Shibasaka, Mineo; Yamamoto, Yoko; Matsumoto, Hideaki; Sasakawa, Hideo

doi:10.1080/00380768.1995.10419566

Cited by 22 publications

(14 citation statements)

References 20 publications

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“…). A previous study by Yamashita et al () also showed that

{NH}_{4}^{+}

nutrition stimulated PM H + ‐ATPase activity. In addition,

{NH}_{4}^{+}

nutrition also induced the PM H + ‐ATPase activity in some other plants such as maize and sorghum ( Schubert and Yan , ; Zeng et al, ).…”

Section: Discussionmentioning

confidence: 70%

“…Accordingly, it may be hypothesized that the PM H + ‐ATPase is involved in the response of plant cells to

{NH}_{4}^{+}

nutrition. Previous studies have shown that PM H + ‐ATPase activity in barley roots is up‐regulated by

{NH}_{4}^{+}

nutrition ( Yamashita et al, ). In contrast, PM H + ‐ATPase activity in roots of paddy rice that exhibits enhanced growth in the presence of

{NH}_{4}^{+}

, is not up‐regulated by

{NH}_{4}^{+}

nutrition ( Zhu et al., ), suggesting that rice is insensitive to

{NH}_{4}^{+}

nutrition.…”

Section: Introductionmentioning

confidence: 96%

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Involvement of plasma membrane H⁺‐ATPase in the ammonium‐nutrition response of barley roots

Zhang

Ding

et al. 2018

J. Plant Nutr. Soil Sci.

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Barley (Hordeum vulgare L.) is a typical ammonium ( NH4 + )‐sensitive crop species and exhibits a futile‐ NH4 + efflux from root cells. Plasma membrane (PM) H+‐ATPase is responsible for pumping H+ out of plant cells and providing the driving force for the transport of various substances. We hypothesized that PM H+‐ATPase is involved in this NH4 + efflux process of barley roots. Barley plants were cultivated in hydroponic solution with 1.0 mM NH4 + or 1.0 mM nitrate ( NO3 - ) as the sole nitrogen source. Plasma membrane vesicles were isolated from the root tips for analyses of the activity. Plasma membrane H+‐ATPase was tested by Western Blot. The transcription of PM H+‐ATPase genes was analyzed using qRT‐PCR. Proton and NH4 + efflux from barley roots was analyzed by scanning ion‐selective electrode technique. The results show that root and shoot growth was repressed by NH4 + nutrition when compared to NO3 - nutrition. Plasma membrane H+‐ATPase activity of barley roots under NH4 + nutrition was significantly higher than that under NO3 - nutrition. Methyl‐ammonium (MeA), a non‐metabolizable analog of NH4 + , had no significant effect on PM H+‐ATPase activity. The enhanced PM H+‐ATPase activity by NH4 + nutrition was consistent with a higher abundance of PM H+‐ATPase protein and higher transcription levels of six PM H+‐ATPase genes. By using the pharmacological agents vanadate and fusicoccin, a close linkage was observed between H+ efflux and NH4 + efflux from barley roots. Taken together, NH4 + nutrition stimulates PM H+‐ATPase activity, and this stimulation is possibly triggered by NH4 + assimilation in barley roots. NH4 + efflux from barley roots is associated with PM H+‐ATPase activity which could provide the driving force for NH4 + efflux. Ammonium efflux might be considered as a disposal strategy in barley roots which prevents NH4 + overloading the root cytoplasm.

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“…). A previous study by Yamashita et al () also showed that

{NH}_{4}^{+}

nutrition stimulated PM H + ‐ATPase activity. In addition,

{NH}_{4}^{+}

nutrition also induced the PM H + ‐ATPase activity in some other plants such as maize and sorghum ( Schubert and Yan , ; Zeng et al, ).…”

Section: Discussionmentioning

confidence: 70%

“…Accordingly, it may be hypothesized that the PM H + ‐ATPase is involved in the response of plant cells to

{NH}_{4}^{+}

nutrition. Previous studies have shown that PM H + ‐ATPase activity in barley roots is up‐regulated by

{NH}_{4}^{+}

nutrition ( Yamashita et al, ). In contrast, PM H + ‐ATPase activity in roots of paddy rice that exhibits enhanced growth in the presence of

{NH}_{4}^{+}

, is not up‐regulated by

{NH}_{4}^{+}

nutrition ( Zhu et al., ), suggesting that rice is insensitive to

{NH}_{4}^{+}

nutrition.…”

Section: Introductionmentioning

confidence: 96%

Involvement of plasma membrane H⁺‐ATPase in the ammonium‐nutrition response of barley roots

Zhang

Ding

et al. 2018

J. Plant Nutr. Soil Sci.

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“…The plasma membrane vesicles were prepared from barley roots and leaves as described by Yamashita et al (1995). The assay medium basically consisted of 30 mM 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES)‐bis‐tris propane (BTP) (pH 7.0), l mM ethylene glycol‐bis(β‐aminoethyl ether)‐ N , N , N ′, N ′‐tetraacetic acid (EGTA)‐BTP, 1 mM Na 2 MoO 4 , 50 mM KCl, 0.1 g L −1 Brij 58, 3 mM MgSO 4 , and 3 mM ATP‐BTP.…”

Section: Methodsmentioning

confidence: 99%

Enhancement of Germination, Seedling Growth, and Oxidative Metabolism of Barley under Simulated Acid Rain Stress by Exogenous Trehalose

Ding

Wang

2018

Crop Science

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This study investigated the effects of simulated acid rain on germination, seedling growth, and oxidative metabolism in barley (Hordeum vulgare L.). Barley seeds were separately soaked in trehalose solutions of 5, 10, and 15 mM concentration or distilled water (the control). Results showed inhibited seed germination, increased foliar damage, decreased chlorophyll content, damaged roots, and delayed seedlings growth in barley when exposed to acid rain at pH 3.0. Although the treatment with acid rain increased the activities of peroxidase, catalase, and plasma membrane H+‐ATPase, membrane permeability and malondialdehyde content still increased significantly. However, the addition of exogenous trehalose significantly alleviated the negative effect of acid rain on growth inhibition of barley and increased total leaf chlorophyll due to an increase of chlorophyll a content. Trehalose also increased catalase and peroxidase activity, which led to an increase in the antioxidant capacity and a balance in production and scavenging of free radicals. Plasma membrane H+‐ATPase activities of roots and leaves also increased with trehalose and maintained stable pH of the cytoplasm of roots and leaf cells under low pH. The 10 mM trehalose pretreatment was enough to alleviative the negative effects of acid rain in barley.

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“…-ATPase has been proved to be involved in salinity stress (Niu et al 1993(Niu et al , 1996, P deficiency (Yan et al 2002), low pH (Zhu et al 2009) and ammonium stress (Yamashita et al 1995). Therefore, it seems that PM H ?…”

Section: Al Independently Regulates Pm H ? -Atpase Activity and Oxalamentioning

confidence: 98%

Aluminum regulates oxalate secretion and plasma membrane H+-ATPase activity independently in tomato roots

Yang

Zhu

Peng

et al. 2011

Planta

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We demonstrated that aluminum (Al)-induced oxalate secretion and plasma membrane (PM) H(+)-ATPase activity in tomato (Lycopersicon esculentum 'Hezuo903') roots were poorly correlated. In addition, vanadate, an inhibitor of PM H(+)-ATPase, had no effect on Al-induced oxalate secretion, but significantly inhibited enzyme activity. An anion channel inhibitor phenylglyoxal inhibited oxalate secretion, but not PM H(+)-ATPase activity. Exposure of tomato roots to 10 μM LaCl(3) also stimulated PM H(+)-ATPase activity; however, La failed to induce oxalate secretion. Furthermore, Al-induced changes of PM H(+)-ATPase activity were not associated with oxalate secretion in two tomato cultivars differing in the ability to secrete oxalate under Al stress. These results indicate that Al independently regulates oxalate secretion and PM H(+)-ATPase activity in tomato roots. Analysis of expression levels of PM H(+)-ATPase genes by real-time reverse transcription-PCR and protein by Western blot and immunodetection revealed that the regulation of PM H(+)-ATPase in response to Al was subjected to transcriptional and posttranscriptional control. However, since neither transcriptional level of genes nor translational level of proteins directly relate to the enzyme activity, posttranslational modification of PM H(+)-ATPase under Al stress likely contributes to changes in activity of this protein.

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Stimulation of H⁺extrusion and plasma membrane H⁺-ATPase activity of barley roots by ammonium-treatment

Cited by 22 publications

References 20 publications

Involvement of plasma membrane H⁺‐ATPase in the ammonium‐nutrition response of barley roots

Involvement of plasma membrane H⁺‐ATPase in the ammonium‐nutrition response of barley roots

Enhancement of Germination, Seedling Growth, and Oxidative Metabolism of Barley under Simulated Acid Rain Stress by Exogenous Trehalose

Aluminum regulates oxalate secretion and plasma membrane H+-ATPase activity independently in tomato roots

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Stimulation of H+extrusion and plasma membrane H+-ATPase activity of barley roots by ammonium-treatment

Cited by 22 publications

References 20 publications

Involvement of plasma membrane H+‐ATPase in the ammonium‐nutrition response of barley roots

Involvement of plasma membrane H+‐ATPase in the ammonium‐nutrition response of barley roots

Enhancement of Germination, Seedling Growth, and Oxidative Metabolism of Barley under Simulated Acid Rain Stress by Exogenous Trehalose

Aluminum regulates oxalate secretion and plasma membrane H+-ATPase activity independently in tomato roots

Contact Info

Product

Resources

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Stimulation of H⁺extrusion and plasma membrane H⁺-ATPase activity of barley roots by ammonium-treatment

Involvement of plasma membrane H⁺‐ATPase in the ammonium‐nutrition response of barley roots

Involvement of plasma membrane H⁺‐ATPase in the ammonium‐nutrition response of barley roots