Stimulation of growth and mucopolysaccharide synthesis by insulin treatment of chick embryo chondrocytes in cell culture

Hajek, Anthony S.; Solursh, Michael

doi:10.1016/0016-6480(75)90154-9

Cited by 29 publications

(3 citation statements)

References 27 publications

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“…Insulin stimulates synthesis of GAGs, has little effect on collagen synthesis and inhibits cartilage differentiation, preventing attainment of chondrocyte hypertrophy (Box 26.1) 11 . Some skeletal aspects of the insulin-based disease diabetes are provided in Box 26.1.…”

Section: Synthesis Of Collagen and Cs Are Regulated Independentlymentioning

confidence: 99%

Maintaining Differentiated Chondrocytes Through Cell–Matrix Interactions

Hall

2015

Bones and Cartilage

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Section: Synthesis Of Collagen and Cs Are Regulated Independentlymentioning

confidence: 99%

Maintaining Differentiated Chondrocytes Through Cell–Matrix Interactions

Hall

2015

Bones and Cartilage

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“…The anabolic effect of insulin on connective tissue and its role as a potent growth factor for connective tissue has been described [ 11 , 12 , 13 ]. Early studies performed on whole animals, organs or tissues indicated that insulin is required for the optimal production of sulfated mucopolysaccharides and collagen, two major components of connective tissue [ 14 , 15 , 16 , 17 ]. In experiments conducted on articular cartilage explants harvested from patients undergoing knee joint replacement, Cai et al [ 11 ] reported that insulin inhibited nitric oxide production and stimulated matrix synthesis by counteracting the deleterious effect of interleukin 1 (IL-1), high levels of which are usually found in arthritic joints.…”

Section: Introductionmentioning

confidence: 99%

Knee Osteoarthritis in Type 2 Diabetes Mellitus: Does Insulin Therapy Retard Osteophyte Formation?

et al. 2015

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Objective: The aim of this study was to investigate whether or not radiographic changes observed in knee osteoarthritis (OA) in type 2 diabetes mellitus (T2DM) patients on insulin therapy differed from those not on insulin. Material and Methods: A cross-sectional study was performed in 311 subjects: 211 T2DM patients and 100 without diabetes (controls) in Mubarak Hospital, Kuwait. Patients were categorized into 3 groups: T2DM patients not on insulin (G1, n = 99), T2DM patients on insulin (G2, n = 112) and a nondiabetic control group (G3, n = 100). Plain X-ray of both knees was used to assess the changes of knee OA and graded using the Kellegren-Lawrence scale (0-4) and the Osteoarthritis Research Society International Atlas grading scale (0-3). A total of 622 knee X-rays were evaluated. SPSS version 21.0 was used for data analysis. Results: A highly significant association (p < 0.0001) was observed for joint space narrowing (JSN) as well as for osteophyte formation between the three groups. Comparing G2 and G3, a highly significant association (p < 0.0001) was retained for JSN [201 (89.7%) vs. 199 (99.5%)] and for osteophyte formation [26 (11.7%) vs. 72 (36.0%)]. Comparing G1 and G2, significantly less osteophyte formation was noted in G2 patients compared to G1 patients [26 (11.7%) vs. 39 (19.7%), p = 0.02]. Multivariate logistic regression analysis showed that the G2 group had less chance of osteophyte formation than either the G1 group or G3 control group (OR = 0.294, p = 0.008 and OR = 0.098, p < 0.001, respectively). Conclusion: Our findings show that T2DM patients with OA knees on insulin therapy have less radiographic osteophytes compared to T2DM patients not on insulin.

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“…In other respects, insulin causes a positive nitrogen balance by elevating amino acid uptake. 20,21 Insulin binds to receptors of insulin-like growth factor 1 (IGF-1) at high concentrations such as 10 µg/mL, and imitates its effect in cartilage tissue. 22 When used at dosages below 10 nmol/L, it causes an increased matrix synthesis while preventing matrix breakdown by inhibiting aggrecanase activity and harmful effects of nitric oxide and interleukin-1 (IL-1) on cartilage tissue.…”

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confidence: 99%

ADAMTS12 Depletion by Insulin in OUMS-27 Human Chondrosarcoma Cells

et al. 2015

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Objectives:In this study, we aim to investigate the association between articular damage in diabetes and a disintegrin-like and metalloproteinase with thrombospondin motifs 12 (ADAMTS12) at gene expression and protein levels. Materials and methods: OUMS-27 human chondrosarcoma cells were used to investigate how ADAMTS12 levels changed in vitro condition in presence and absence of insulin. The study included three groups of cells treated with 10 μg/mL of insulin, and a control group. Cells were incubated with insulin in medium for one day, three days, and seven days. The effects of insulin on ADAMTS12 were investigated at both gene expression and protein levels. The relationships between the variables were tested by Mann-Whitney U test. Results: ADAMTS12 expression was significantly lower in the groups treated with insulin medium for one day and seven day periods (p=0.008 and p=0.008, respectively) compared to the control group. No significant difference was detected in the expression level between the groups kept in insulin medium for three days and the control group (p=0.55). In addition, protein amounts of the groups exposed to insulin medium for one, three, and seven day periods were lower. Conclusion: Insulin reduces the amount of ADAMTS12 which causes delayed recovery of cartilage tissue in the OUMS-27 cell lines utilized in our study for their chondrocytic properties. This reduction due to insulin treatment may contribute to recovery of cartilage tissue.

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Stimulation of growth and mucopolysaccharide synthesis by insulin treatment of chick embryo chondrocytes in cell culture

Cited by 29 publications

References 27 publications

Maintaining Differentiated Chondrocytes Through Cell–Matrix Interactions

Maintaining Differentiated Chondrocytes Through Cell–Matrix Interactions

Knee Osteoarthritis in Type 2 Diabetes Mellitus: Does Insulin Therapy Retard Osteophyte Formation?

ADAMTS12 Depletion by Insulin in OUMS-27 Human Chondrosarcoma Cells

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