Stimulation of granulocytic colony formation in agar diffusion chambers implanted in cyclophosphamide pretreated mice

Gordon, M. Y.; Blackett, N. M.

doi:10.1038/bjc.1975.133

Cited by 20 publications

(9 citation statements)

References 18 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One of the MGI-D-clones used, clone 6, can be induced for Fc rosettes by dexamethasone (14). There appear to be different cellular sites for induction by different compounds (10,11,14 (25,26) by injection of cyclophosphamide. The enhancement of the growth of cells associated with inhibition of differentiation in these mice could result from inhibition of differentiation and possibly also from changes in the levels of other stimulators or inhibitors of growth (42).…”

mentioning

confidence: 99%

In vivo induction of normal differentiation in myeloid leukemia cells

Lotem¹,

Sachs²

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

MGI+D+, MGI+D-, and MGI-D-mouse myeloid leukemic cells, which genetically differ in their competence to be inducbd to undergo normal cell differentiation in vitro by the normal macrophage-and anulocyte-inducing irotein MGI, were analyzed for their afility to undergo cell differentiation in diffusion chambers in vivo. As after induction by MGI in vitro, MGI+D+ clones were induced for Fc and C3 rosettes, lysozyme, and mature macrophages and granulocytes in normal syngeneic or allogeneic mice. MGI+D-clones were also induced in these mice for all these properties, although in vitro they were not induced by MGI for mature cells. The MGI-D-clones were induced in vivo for C3 and Fc rosettes, lysozyme, and intermediate stages but not for mature cells, whereas none of these properties were induced in these clones by MGI in vitro. Thus, certain types of myeloid leukemic cells differentiate better in vivo, possibly due to the presence of higher effective concentrations of MGI and/or other inducing factors, and MGI+D+ and MGI+D-cells can completely differentiate in vivo to mature cells. In vivo differentiation was inhibited in mice treated with cyclophosphamide. It was also inhibited in various strains of nude mice, except for one MGI+D+ clone, where it was inhibited in C57BL/6 but not in ICR nude mice. This MGI+D+ clone was also the only clone that was induced to differentiate normally in vitro by a 23,000 molecular weight form of purified MGI. The results suggest that different clones respond to different molecular forms of MGI, which may be present in different proportions in some animals, that in vivo differentiation by MGI possibly with other factors may be regulated by cells involved in the immune response, and that this differentiation can be genetically controlled. Differentiation in vivo was enhanced by injection of conditioned medium containing MGI and by inoculation of MGI-roducing cells, including normal granulocytes. This indicates that the induction of normal differentiation of myeloid leukemic cells in vivo can be enhanced by these treatments. We have developed an in vitro system to study the controls that regulate growth and differentiation of normal (1-6) and leukemic (5-11) myeloid cells. The use of this system has shown that the normal macrophage-and granulocyte-inducing protein (2-4), which we now call MGI (6, 12), can induce in vitro normal precursor cells (2-6, 13) and some types of myeloid leukemic cells in mice (7-11) and humans (5) to undergo complete differentiation to mature macrophages or granulocytes. Mouse myeloid leukemic cell clones have been established that can be grown in culture as myeloblasts to promyelocytes, have a high leukemogenic capacity, and express different genetic blocks in differentiation (8-11). Some of these clones (MGI+D+) can be induced in vitro by purified MGI (7) to undergo differentiation to mature macrophages and granulocytes. This differentiation occurs via the normal differentiation sequence of induction of C3 and Fc rosettes (9, 14), C3-and Fc-mediated immu...

show abstract

mentioning

confidence: 99%

In vivo induction of normal differentiation in myeloid leukemia cells

Lotem¹,

Sachs²

1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…The Agar diffusion chamber technique of Gordon and Blackett [14] was used. Diffusion chambers (DC) were prepared by attaching membrane filters (Sartorius, 0.45 gm pore size) to each side of lucid rings.…”

Section: Culture Systemmentioning

confidence: 99%

Granulocyte progenitors (CFU-D) in neutrophilic leukemoid reaction are hyporesponsive to macrophage-induced inhibition

Hansz¹,

Sawiński²

1988

Res. Exp. Med.

View full text Add to dashboard Cite

The responsiveness of marrow granulocyte progenitors (CFU-D) to macrophage-derived stimulatory and inhibitory factors has been studied using diffusion chamber technique in 12 patients with neutrophilic leukemoid reaction (with granulocyte count in the range between 10-40 G/l) and ten healthy subjects. CFU-D from patients with neutrophilic leukemoid reaction (NLR) revealed a normal reactivity to colony-stimulating activity, whereas they were hyporesponsive to macrophage-derived indomethacin-sensitive inhibition. This altered response was correlated both with the concentration of granulocyte progenitors in the S phase and with blood neutrophilic leukocytosis. In patients with higher granulocyte count and increased concentration of CFU-D during active DNA synthesis a more pronounced hyporesponsiveness of granulocyte progenitors to macrophage-induced inhibition has been found.

show abstract

“…Certain manipulations of the host animal cause stimulation or suppression of D C granulopoiesis through humoral mechanisms. Prior treatment of the host with irradiation (B0yum et al, 1972;Rothstein et al, 1971) or cyclophosphamide (Gordon & Blackett, 1975;Tyler et al, 1972) is predictably associated with augmentation of GMM proliferation within DC. Conversely, humoral substances from intact or disintegrated bone marrow cells (Rothstein et al, 1973), neutrophils (Bsyum et al, 1976 and neutrophilic extracts (Lovhaug & Brayum, 1977) have been convincingly demonstrated to suppress D C granulopoiesis.…”

mentioning

confidence: 99%

The Role of Colony Stimulating Activity in Modulating Murine Diffusion Chamber Granulopoiesis

1980

View full text Add to dashboard Cite

We studied the effects of high circulating Colony Stimulating Activity (CSA) levels and irradiation induced marrow hypoplasia in CF1 and C57B1/6J host mice upon granulopoiesis in intraperitoneal diffusion chamber (DC) cultures. Serial endotoxin injections resulted in marked elevation of circulating CSA for the first half of an 8 d culture period, and CSA was shown to diffuse into the chamber environment; yet this manipulation alone did not significantly accelerate DC cell growth. Pre-irradiation of the host mice produced no elevation of circulating CSA during the early phase of culture, but resulted in significant stimulation of DC granolopoiesis. Fluctuations in circulating inhibitors of in vitro granulopoiesis did not correlate well with DC cellularity. We conclude that endogenous CSA elevation does not providean effective stimulus per se for granulocyte-monocyte proliferation within DC culture and cannot be solely responsible for mediating the exuberant DC granulopoietic response seen in the pre-irradiated host.

show abstract

Stimulation of granulocytic colony formation in agar diffusion chambers implanted in cyclophosphamide pretreated mice

Cited by 20 publications

References 18 publications

In vivo induction of normal differentiation in myeloid leukemia cells

In vivo induction of normal differentiation in myeloid leukemia cells

Granulocyte progenitors (CFU-D) in neutrophilic leukemoid reaction are hyporesponsive to macrophage-induced inhibition

The Role of Colony Stimulating Activity in Modulating Murine Diffusion Chamber Granulopoiesis

Contact Info

Product

Resources

About