Stimulation of Erythrocyte Membrane Blebbing by Naproxen Sodium

Ilyas, Sajida; Jilani, Kashif; Sikandar, Muhammad; Siddiq, Saba; Riaz, Muhammad; Naveed, Ayesha; Bibi, Ismat; Nawaz, Haq; Irfan, Muhammad; Asghar, Asma

doi:10.1177/1559325819899259

Cited by 10 publications

(4 citation statements)

References 29 publications

(45 reference statements)

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“…observed that xenobiotics induced oxidative stress results in membrane blebbing of erythrocytes. 36 Inhibition of the membrane blebbing confirms the role of calcium in the oxidative stress induced eryptosis. Naveed et al, 37 shared the similar information that calcium channel blockage results in a decrease of the mean cell volume of erythrocytes indicating a stoppage of oxidative stress stimulated membrane blebbing.…”

Section: Discussionmentioning

confidence: 79%

Stimulation of Erythrocyte Membrane Blebbing by Bifenthrin Induced Oxidative Stress

et al. 2022

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Background Bifenthrin is an insecticide and anti-estrogenic compound primarily used to control residential pests by depolarizing sodium gated voltage channels in the nervous system. Eryptosis, the suicidal death of erythrocytes, featured by PS exposure, membrane blebbing and cell shrinkage. Anemia is an outcome of uncontrolled eryptosis. Research Design In this study, erythrocytes were treated with different concentrations (.5-1-1.5 μM) of bifenthrin over a period of 48 hours. In order to investigate the oxidative stress induced by bifenthrin, catalase, superoxide dismutase, and glutathione peroxidase activities were investigated. Results Obtained data indicated the decrease in the enzymes (superoxide dismutase, glutathione peroxidase, and catalase) activities in bifenthrin treated cells at 1 μM concentration. In addition, measurement of cell size and confirmation of the role of calcium in the stimulation of the eryptotic activity of bifenthrin were performed. A significant increase in mean cell volume was found in the presence of bifenthrin and a decrease in mean cell volume in the presence of calcium channel blocker was observed. Similarly, there was also a significant increase in the percentage of hemolysis indicating the necrotic activity of bifenthrin. Conclusions It is concluded that the indicated doses of bifenthrin triggered oxidative stress which may lead to early cell death by eryptosis and hemolysis.

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Section: Discussionmentioning

confidence: 79%

Stimulation of Erythrocyte Membrane Blebbing by Bifenthrin Induced Oxidative Stress

et al. 2022

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“…Oxidative stress is a reported mechanism of eryptosis and variation among anti-oxidative enzymes in an important indicator of oxidative stress. 23 Superoxide dismutase enzyme catalyzes the dismutation of O 2 free radicals that on accumulation may results in lowering the superoxide dismutase’s level and mitochondrial dysfunction resulting in oxidative stress. 24 Catalase is an iron-dependent enzyme that may act peroxidatively or catalytically 24 and considered as a major antioxidant enzyme.…”

Section: Discussionmentioning

confidence: 99%

Induction of Erythrocyte Shrinkage by Omeprazole

et al. 2020

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Omeprazole, a proton pump inhibitor blocks the H+/K+-ATPase channels of gastric parietal cells. It is used for the treatment of peptic ulcer. Prolonged use of omeprazole may involve in inducing anemia. The key marker of eryptosis includes membrane blebbing, cell shrinkage and phosphatidylserine (PS) exposure at the cell surface. In current study, the eryptotic, oxidative as well as hemolytic effects of therapeutical doses (0.5, 1 and 1.5 µM) of omeprazole were investigated after exposing erythrocytes for 48 hours. Investigation of eryptosis was done by cell size measurement, PS exposure determination and calcium channel inhibition. As a possible mechanism of omeprazole induced eryptosis, oxidative stress was investigated by determining the catalase, glutathione peroxidase and superoxide dismutase activities. Similarly, necrotic effect of omeprazole on erythrocytes was also evaluated through hemolysis measurement. Results of our study illustrated that 1.5 µM of omeprazole may induce significant decrease in superoxide dismutase, glutathione peroxidase and catalase activities as well as triggered the erythrocytes shrinkage, PS exposure and hemolysis. Role of calcium was also confirmed in inducing erythrocyte shrinkage. It is concluded that the exposure of erythrocytes with 1.5 µM omeprazole may enhance the rate of eryptosis and hemolysis by inducing oxidative stress.

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“…The activity of glutathione peroxidase was determined by adding 50 mM phosphate buffer (pH 5), 20 mM guaiacol, 0.1 mL enzyme extract, and 40 mM H 2 O 2 in the reaction solution according to the method used by Ilyas et al 37 Enzyme activity was measured at 470 nm after every 20Sec.…”

Section: Methodsmentioning

confidence: 99%

“…MCV was estimated by using automated hematology analyzer KX-21 Sysmex, Japan. 37,38 Confirmation of the Role of Calcium in Membrane Blebbing…”

Section: Measurement Of Cell Sizementioning

confidence: 97%

Induction of Erythrocyte Membrane Blebbing by Methotrexate-Induced Oxidative Stress

et al. 2022

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Methotrexate (MTX) is a common chemotherapeutical agent and folate antagonist with reported apoptotic activity in nucleated cells. The presented research work was planned to investigate the eryptotic effects of methotrexate after the exposure of erythrocytes to therapeutical doses (10–15 μM) of methotrexate. Eryptosis and the role of calcium in the stimulation of membrane blebbing were evaluated through the determination of mean cell volume. Oxidative stress induced by methotrexate (10–15 μM) was determined by antioxidative enzyme activities. Cytotoxic activity against human erythrocytes was examined through hemolysis assay. Exposure of erythrocytes to methotrexate results in significant reduction of superoxide dismutase, catalase, and superoxide dismutase activities at 10 and 15 μM in comparison to the untreated cells. Erythrocytes mean cell volume (MCV) was increased after 48 hours exposure of erythrocytes to methotrexate (10 μM). Significantly increased hemolysis percentage was observed at 10 μM after 48 hours incubation of erythrocytes with methotrexate. The results of the study suggested that the therapeutical doses (10–15 μM) of methotrexate may lead to increase in eryptotic and hemolytic activity of erythrocytes through free radical generation and subsequent calcium entry.

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Stimulation of Erythrocyte Membrane Blebbing by Naproxen Sodium

Cited by 10 publications

References 29 publications

Stimulation of Erythrocyte Membrane Blebbing by Bifenthrin Induced Oxidative Stress

Stimulation of Erythrocyte Membrane Blebbing by Bifenthrin Induced Oxidative Stress

Induction of Erythrocyte Shrinkage by Omeprazole

Induction of Erythrocyte Membrane Blebbing by Methotrexate-Induced Oxidative Stress

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