Stimulation of endothelial cell migration in culture by ladsin, a laminin-5-like cell adhesion protein

Abstract

Ladsin is a laminin-like cell-adhesive scatter factor with potent cell motility-stimulating ability and was purified from serum-free conditioned medium of a malignant human gastric adenocarcinoma cell line STKM-1. To test its possible role in tumor angiogenesis, we investigated its effect on primary culture of endothelial cells (human umbilical vein endothelial cells) and endothelial cell line ECV304 in this study. Cell adhesion and motility effects of ladsin were observed in both types of endothelial cells. In cell-attachment assay, ladsin interacted with integrin alpha 3 beta 1 that was expressed on the endothelial cell surface. In Boyden chambers, ladsin stimulated both directed and random migration of ECV304 cells. Ladsin induced repair of artificial wounds generated in ECV304 cell monolayers by stimulating cell migration. Ladsin did not affect the growth rate of ECV304 cells at a low cell density but significantly increased the saturation cell density. These results suggest that ladsin may be involved in the adhesion and migration of endothelial cells under some physiological and pathological conditions.

Isolation and Characterization of Laminin-10/11 Secreted by Human Lung Carcinoma Cells

“…hesive properties. Laminin-5 has been reported to be most potent in mediating adhesion of keratinocytes (32), endothelial cells (36), and glioma cells (12) among various adhesive proteins including laminin-1, laminin-2/4, fibronectin, and vitronectin. Our results showed that laminin-10/11 has potency comparable to that of laminin-5 in mediating cell adhesion to the substratum.…”

Sole Expression of Laminin γhain in Invading Tumor Cells and Its Association with Stromal Fibrosis in Lung Adenocarcinomas

“…[7][8][9] LN-5 strongly promotes adhesion, migration, and scattering of various types of cultured cells compared with other extracellular matrix proteins. 4,10,11) The expression of LN-5 in tumor cells is stimulated by growth factors and a tumor promoter in vitro. 12,13) Therefore, LN-5 has been supposed to play some roles in tumor invasion and metastasis.…”

Cell-cell contacts in the human cell line ECV304 exhibit both endothelial and epithelial characteristics

“…It is a commonly used in vitro model system for endothelial cells. For example, ECV304 cells have been used for studies of angiogenesis (Hughes 1996), cell migration (Kikkawa et al 1996), cytokine expression (Villarete and Remick 1995), or signal transduction of vascular endothelial growth factor (Abedi and Zachary 1997). As a permanent cell line, ECV304 cells offer several advantages over primary cultured endothelial cells, including uniform batches, which allow more valid comparisons between results from different laboratories.…”