Stimulation of cAMP accumulation by the cloned Xenopus melatonin receptor through Gi and Gz proteins

Yung, Lisa Y.; Tsim, Siu-Tai; Wong, Yung Hou

doi:10.1016/0014-5793(95)00963-a

Cited by 64 publications

(55 citation statements)

References 25 publications

(41 reference statements)

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“…These receptors are encoded by separate genes (Reppert et al 1994(Reppert et al , 1995(Reppert et al , 1996. Both melatonin receptors inhibit adenylyl cyclase via pertussis toxin-sensitive Gi proteins (Yung et al 1995;Barret et al 1999). The MT 1 receptor is localized in the hypothalamic SCN and hypophyseal pars tuberalis (Sugden et al 1999; revised by Dubocovich et al 2003).…”

mentioning

confidence: 99%

In vivo activation of insulin receptor tyrosine kinase by melatonin in the rat hypothalamus

Anhê

Caperuto

Pereira-da-Silva

et al. 2004

Journal of Neurochemistry

106

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Melatonin is the pineal hormone that acts via a pertussis toxinsensitive G-protein to inhibit adenylate cyclase. However, the intracellular signalling effects of melatonin are not completely understood. Melatonin receptors are mainly present in the suprachiasmatic nucleus (SCN) and pars tuberalis of both humans and rats. The SCN directly controls, amongst other mechanisms, the circadian rhythm of plasma glucose concentration. In this study, using immunoprecipitation and immunoblotting, we show that melatonin induces rapid tyrosine phosphorylation and activation of the insulin receptor b-subunit tyrosine kinase (IR) in the rat hypothalamic suprachiasmatic region. Upon IR activation, tyrosine phosphorylation of IRS-1 was detected. In addition, melatonin induced IRS-1/PI(3)-kinase and IRS-1/SHP-2 associations and downstream AKT serine phosphorylation and MAPK (mitogen-activated protein kinase) phosphorylation, respectively. These results not only indicate a new signal transduction pathway for melatonin, but also a potential cross-talk between melatonin and insulin.

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mentioning

confidence: 99%

In vivo activation of insulin receptor tyrosine kinase by melatonin in the rat hypothalamus

Anhê

Caperuto

Pereira-da-Silva

et al. 2004

Journal of Neurochemistry

106

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“…Among the other known GPCRs expressed in the SCN, the receptors for serotonin (5-HT1A) and melatonin (MT1 and MT2) are also able to couple to Gz [33][34][35][36], extending a potential contribution of Gz to the circadian clock regulatory mechanisms.…”

Section: Gpr176 Couples To Gzmentioning

confidence: 99%

G-protein-coupled receptor signaling through Gpr176, Gz, and RGS16 tunes time in the center of the circadian clock [Review]

et al. 2017

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Abstract. G-protein-coupled receptors (GPCRs) constitute an immensely important class of drug targets with diverse clinical applications. There are still more than 120 orphan GPCRs whose cognate ligands and physiological functions are not known. A set of circadian pacemaker neurons that governs daily rhythms in behavior and physiology resides in the suprachiasmatic nucleus (SCN) in the brain. Malfunction of the circadian clock has been linked to a multitude of diseases, such as sleeping disorders, obesity, diabetes, cardiovascular diseases, and cancer, which makes the clock an attractive target for drug development. Here, we review a recently identified role of Gpr176 in the SCN. Gpr176 is an SCN-enriched orphan GPCR that sets the pace of the circadian clock in the SCN. Even without known ligand, this orphan receptor has an agonist-independent basal activity to reduce cAMP signaling. A unique cAMP-repressing G-protein subclass Gz is required for the activity of Gpr176. We also provide an overview on the circadian regulation of G-protein signaling, with an emphasis on a role for the regulator of G-protein signaling 16 (RGS16). RGS16 is indispensable for the circadian regulation of cAMP in the SCN. Developing drugs that target the SCN remains an unfulfilled opportunity for the circadian pharmacology. This review argues for the potential impact of focusing on GPCRs in the SCN for the purpose of tuning the body clock.

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“…These results suggested that melatonin has enhanced the regulatory role of Ca 2+ /calmodulin pathway in sperm motility and velocity parameters including VCL and ALH in liquid storage of mithun semen. Moreover, melatonin has improved the mobility and velocity through act on the cyclic AMP (cAMP) stimulator (Yung et al, 1995) and cAMP stimulates sperm motility via its direct action on the axoneme of the tail (Lindamann, 1978) or indirectly through acting on the cell membrane as secondary messenger (Garbers and Kopf, 1980). Thus the melatonin treated sperm has higher motility and velocity parameters in the present study.…”

Section: Advances In Animal and Veterinary Sciencesmentioning

confidence: 99%

“…It has been reported that melatonin has potency twice against peroxyl radicals as that of vitamin E in action (Pieri et al, 1994) and also it is more effective in scavenging of the hydroxyl radicals than mannitol and reduced glutathione (Hardeland et al, 1993). Melatonin is associated with mitochondrial membrane potential (Acuna-Castroviejo et al, 2011), ATP production (Chen et al, 1994), interaction with calmodulin (Tash et al, 1983), stimulates cellular influx of Ca2+ into sperm cells (Delgadillo et al, 1994) and cyclic AMP (cAMP) stimulator (Yung et al, 1995), through which the melatonin stimulate the sperm to get higher motility and velocity.…”

Section: Introductionmentioning

confidence: 99%

Casa Parameters of Mithun Semen Treated with Melatonin

Perumal¹

2016

Adv. Anim. Vet. Sci.

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| A study was conducted to measure the effect of melatonin (MT) on motility and velocity parameters of mithun semen by Computer Assisted Sperm Analyser (CASA) in different seasons. Total numbers of 80 ejaculates (20 ejaculates in each season) were collected twice a week from mithun bulls and were split into five equal aliquots, diluted with the Tris Egg Yolk Citrate (TEYC) extender. Group 1: semen without additives (control), group 2 to group 6: semen was diluted with 1, 2, 3, 4 and 5 mM of melatonin, respectively. CASA parameter such as Forward Progressive Motility (FPM), Total Motility (TM), Curvilinear Velocity (VCL), Straight Line Velocity (VSL), Average Path Velocity (VAP), Linearity (LIN), Straightness (STR), Amplitude of Lateral Head displacement (ALH) and Beat / Cross Frequency (BCF) were measured after 12 hrs of incubation at 5 o C. Blood samples were collected in different seasons to estimate the melatonin. The results revealed that these parameters were varied significantly (p<0.05) among the different experimental groups and among the seasons. Further, MT at 3 mM has significant improvement than other treatment groups stored in in-vitro at different seasons. Additionally, spring season has highest value followed by winter and autumn season, whereas lower values were in summer season. Melatonin concentration was higher in winter season and lowest was in summer season. The result of the study indicated that the melatonin protects CASA parameters, varied in different seasons. It was concluded that ejaculates from spring and winter season has significantly higher CASA parameters in mithun. Keywords

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Stimulation of cAMP accumulation by the cloned Xenopus melatonin receptor through G_i and G_z proteins

Cited by 64 publications

References 25 publications

In vivo activation of insulin receptor tyrosine kinase by melatonin in the rat hypothalamus

In vivo activation of insulin receptor tyrosine kinase by melatonin in the rat hypothalamus

G-protein-coupled receptor signaling through Gpr176, Gz, and RGS16 tunes time in the center of the circadian clock [Review]

Casa Parameters of Mithun Semen Treated with Melatonin

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