Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat

Abstract

Objective: The aim of this study was to evaluate the effects of vitamin E (α-tocopherol) administration on bone formation in response to expansion of the inter-premaxillary suture, in rats, histomorphometrically. Methods: Thirty 50 -60 day old Wistar rats were separated into five equal groups (one control and four experimental). All groups were subjected to inter-premaxilla expansion with 50-gram of force. Six control animals received saline solution (Group I) and three experimental groups were treated with a single dose of α-tocopherol injected into the inter-premaxillary suture after one day after appliance placement (Group II: 2 mg/kg; Group III: 10 mg/kg; and Group IV: 50 mg/kg). A further group of six animals received three injections of 10 mg/kg α-tocopherol, one each on days 3, 6, and 9 (Group V). Bone formation in the suture was evaluated by bone histomorphometry. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p ＜ 0.05 level. Results: New bone area, bone perimeter, feret's diameter and newly formed bone measurements were significantly higher in the experimental groups than the control (p ＜ 0.001). Bone architecture in α-tocopherol administrated groups was improved, and bone formation during the expansion period was stimulated significantly, in a dose-dependent manner. Conclusions: The application of α-tocopherol during the early stages to orthopedically expanded inter-premaxillary suture areas may stimulate bone formation and shorten the retention period, in rats.

Effect of vitamin E on human periodontal ligament fibroblasts

“…It is prudent to take the periodontal status in consideration and the complex response to orthodontic tooth movement which starts with the periodontal ligament fibroblasts (2). Little is known about its effect vitamin E on periodontal tissues but it started gaining some attention of orthodontists over the past few years (12,13).…”

Effect of vitamin C on bone formation in the expanded inter-premaxillary suture. Early bone changes

“…The image analysis program evaluated the histological findings objectively, demonstrating that the dimensions of the bone area correlate with newly-formed bone. Bone histomorphometry is a reliable technique frequently used in the quantitative evaluation of bone remodeling in experimental [14,16,20,24,25,26,27] in vivo and in vitro conditions [4].…”

“…It would thus be potentially beneficial to accelerate bone growth and shorten the retention period in the suture during and after expansion to prevent a relapse in the skeletal base [14,16]. Various clinical and experimental investigations have focused on the acceleration of bone formation and consolidation, thereby aiming to shorten the framing time [14,16,18,22,23,24,25].…”

“…Deswegen wäre es potenziell von Nutzen, die Knochenbildung zu beschleunigen und die Retentionsphase in der Naht während und nach der Expansion abzukürzen, um ein Rezidiv der skelettalen Basis zu vermeiden [14,16]. Verschiedene klinische und experimentelle Studien haben sich schwerpunktmäßig mit der Beschleunigung der Knochenbildung, der Konsolidierung und damit mit einer Verkürzung der Anwendungszeit der kieferorthopädischen Apparatur befasst [14,16,18,22,23,24,25].…”

Stimulation of bone formation by direct electrical current in an orthopedically expanded suture in the rat

“…6 It could be postulated that accelerated bone formation in the suture after expansion may reduce the amount of time required for retention and prevent the maxillae from relapsing. [6][7][8][9][10] Interest in methods of accelerating bone healing persists in many surgical orthopedical concepts. The promise of successful invasive and noninvasive alternatives to accelerate bone healing continues to be realized.…”