Stimulation of asiaticoside accumulation in the whole plant cultures of Centella asiatica (L.) Urban by elicitors

Abstract

The effects of a number of different elicitors on asiaticoside production in whole plant cultures of Centella asiatica were studied, including yeast extract, CdCl(2), CuCl(2) and methyl jasmonate (MJ). Only MJ and yeast extract stimulated asiaticoside production--1.53 and 1.41-fold, respectively. Maximum asiaticoside production was achieved following treatment with 0.1 mM MJ (116.8 mg/l). The highest asiaticoside production (342.72 mg/l) was obtained after 36 days of elicitation in cultures treated with 0.1 mM MJ and 0.025 mg/l 1-phenyl-3-(1,2,3-thidiazol-5-yl)urea (TDZ). Interestingly, MJ not only stimulated the production of asiaticoside but also had an important role in the senescence of C. asiatica. Although asiaticoside content did not change when TDZ was added to medium containing an elicitor, TDZ did increase shoot growth of C. asiatica. We discuss the interactive roles of MJ and TDZ in secondary metabolic production and biomass in whole plants of C. asiatica.

Production of asiaticoside and madecassoside in Centella asiatica in vitro and in vivo

“…The inability to detect asiaticoside and madecassoside in callus is consistent with the observations of Kim et al (2004) who failed to detect asiaticoside in undifferentiated cells of Korean C. asiatica. Plant secondary metabolites are normally synthesised by specialised cells, often at distinct stages of plant development, and certain compounds are not synthesised if cells remain undifferentiated (Kim et al 2002).…”

Cloning and Functional Analysis of a &beta;-Amyrin Synthase Gene Associated with Oleanolic Acid Biosynthesis in <i>Gentiana straminea</i> M<small>AXIM.</small>

“…[30][31][32] However, other data conflict with these results. 33,34) The present data are supportive of the former view, since the GsAS1 transcript was up-regulated by MeJA treatment over a prolonged time period (6 h to 10 d post treatment) and reached a peak of expression at 48 h; in addition, the accumulation of oleanolic acid was at its most rapid at 48 h. These events are consistent with a scenario whereby GsAS1 transcript and the accumulation of its downstream product oleanolic acid are simultane- …”

Accumulation of madecassoside - a major component of centelloside - in centella (Centella asiatica (L.) Urban) cells elicited by salicylic acid

“…There were some reports on in vitro cultures of centella treated by methyl jasmonate (MJ) to improve the biosynthesis of triterpene saponins: for example, stimulation of asiaticoside production in plantlets (5) and hairy-root cultures (6) by MJ, effect of MJ on triterpene metabolisms of planlets (7), centelloside production in cells elicited by MJ (8), and metabolomic analysis of MJ-induced triterpenoid production in cells (9). However, there are no reports on the effects of salicylic acid (SA), a common chemical elicitor in plant (10), on the accumulation of madecassoside in centella cell cultures.…”