Stimulation of aromatase activity in breast fibroblasts by tumor necrosis factor

Development of hormone-dependent prostate cancer models for the evaluation of inhibitors of 17β-hydroxysteroid dehydrogenase Type 3

“…In experiments to monitor hormone-dependent cell proliferation in vitro, cells are often grown in medium containing charcoal-stripped FBS as this removes any steroids usually present in FBS (Macdiarmid et al, 1994). However, as the LNCaP cells were not viable in this medium without supplementation for the duration required for this assay, but were viable without proliferation in medium containing 0.1% FBS, 0.1% FBS-containing medium was used as the basal medium for steroid stimulation assays.…”

Inhibition of IL-6+IL-6 soluble receptor-stimulated aromatase activity by the IL-6 antagonist, Sant 7, in breast tissue-derived fibroblasts

“…The remaining aqueous phase was treated with an equal volume of a solution containing charcoal (5.0%) and dextran (0.5%), centrifuged and an aliquot of the supernatant (1 ml) was taken to determine its radioactive content by liquid scintillation spectrometry. It has previously been established that aromatase activity, as measured in fibroblasts, is linear with respect to time for up to 24 h (Macdiarmid et al, 1994).…”

“…Using such fibroblasts a number of cytokines including interleukin 6 (IL-6) and tumour necrosis factor a (TNFa) were identified as important regulators of fibroblast aromatase activity (Reed et al, 1992;Macdiarmid et al, 1994). Peripheral aromatase activity is increased in elderly and obese subjects (Grodin et al, 1973;Hemsell et al, 1974) and production of IL-6 is also increased in these conditions (Wei et al, 1992;Mohamed-Ali et al, 1997).…”

Vasculitis, Vitiligo, Thyroiditis, and Altered Hormone Levels After Anti-Tumor Necrosis Factor Therapy

“…Aromatase, the enzyme that converts androgens to estrogens, is stimulated by TNF-a, and the early and late elevated levels of estradiol may reflect the level of TNF that existed in this patient prior to infliximab 5 . Inhibitors of TNF decrease the estrogen levels in joint fluids after therapy through a mechanism that involves a decrease in aromatase activity 6 . Estrogens are independent upregulators of immunoglobulin synthesis and the patient's total immunoglobulin of 2238 mg/dl during the time of high estrogen levels reflects that effect 7 .…”