Stimulation of Acid Phosphatase Synthesis and Secretion in Ochromonas danica by Chloramphenicol Base

Aaronson, S.; Patni, N. J.

doi:10.1099/00221287-104-2-181

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…This means that the extent of protein synthesis inhibition depends on the cycloheximide concentration. Based on literature references (McMahon 1975, Aaronson & Patni 1978 we used a 10 ymol 1-' cycloheximide concentration in Expt 1. Comparison of APA development in cycloheximide treated subcultures with control subcultures that contained bacteria only (obtained by filtering an identical subculture through Whatman GF/C glass-fiber filter) indicated that a concentration of 25 pm01 1-' cycloheximide inhibits alkaline phosphatase synthesis in Phaeocystis completely but a cycloheximide concentration of 10 pm01 I-' does not.…”

Section: Methodsmentioning

confidence: 99%

Regulation of alkaline phosphatase synthesis in Phaeocystis sp.

Vanboekel

Veldhuis

1990

Mar. Ecol. Prog. Ser.

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In short-term experiments, cells of Phaeocystis sp. grown under phosphate-sufficient conditions were harvested and transferred to phosphate-depleted medium. In these cultures alkaline phosphatase activity (APA) developed after a lag-phase of 8 h; addition of cycloheximide (10 pm01 I-') partly inhibited APA development. Addition of organic phosphate (AMP, 0.5 pm01 l-l) did not have an inducing effect on alkaline phosphatase synthesis. These results indicate that in Phaeocystis sp, alkaline phosphatase synthesis is controlled by the external phosphate concentration. Synthesis is derepressed at low inorganic phosphate concentration in the medium; the threshold concentration for derepression was ca 0.5 pm01 1-' phosphate. The dark synthesis rate of alkaline phosphatase in Phaeocystis sp. was not correlated with the length of the preceding light period for both colony cells and single cells, although at least a 2 h light period was needed by colony cells to give an enhanced dark synthesis rate compared with a dark control. In a batch culture growth experiment APA developed during the exponential growth phase of Phaeocystis sp. APA development was found to be independent of the initial N/P ratio of the medium. The results imply that APA is not a good indicator of phosphorus limitation in Phaeocystis sp.

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Section: Methodsmentioning

confidence: 99%

Regulation of alkaline phosphatase synthesis in Phaeocystis sp.

Vanboekel

Veldhuis

1990

Mar. Ecol. Prog. Ser.

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Acid phosphatase synthesis inAspergillus flavus

Famurewa

Olutiola

1994

Folia Microbiol

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Proteins with phosphatase activity were produced during the growth of Aspergillus flavus in a phosphate-supplemented liquid synthetic medium. The best carbon and nitrogen sources for the synthesis of phosphatase were glucose and ammonium sulfate, respectively. The proteins were separated by molecular exclusion and ion exclusion chromatography (IEC) into three components one of which showed phosphatase activity. The molar mass of the enzyme was approximately 62 kDa. The purified enzyme exhibited an optimum activity at pH 4.0 and at 45 degrees C. The activity of the enzyme was stimulated by Ca2+ and Mg2+ but inhibited by fluoride, iodoacetic acid, ethylenediaminetetraacetic acid and 2,4-dinitrophenol, and exhibited an apparent KM of approximately 420 mumol/L.

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