Stimulation of 125I-3-iodo-α-methyl-l-tyrosine uptake in Chinese hamster ovary (CHO-K1) cells by tyrosine esters

Shikano, Naoto; Ogura, Masato; Sagara, Jun‐ichi; Nakajima, Syuichi; Kobayashi, Masato; Baba, Takeshi; Yamaguchi, Naoto; Iwamura, Yukio; Kubota, Nobuo; Kawai, Keiichi

doi:10.1016/j.nucmedbio.2009.10.003

Cited by 7 publications

(8 citation statements)

References 24 publications

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“…Similar induction of sugar transport was reported by Miller, who observed accelerated sugar out-flow [21]. Such an acceleration of substrate transport is an obligatory exchange mechanism in amino acid transport [15,22]. Our preliminary experiments, however, did not show acceleration of the out-flow of 14 C-FDG when hexose was present outside the cell, and we hypothesized that augmentation of FDG accumulation is due to elevation in the levels of intracellular glycolytic enzymes, not acceleration in the obligatory exchange.…”

Section: Discussionsupporting

confidence: 86%

“…In this study, 14 tyrosine esters effectively enhance transfer of a radiolabeled L-tyrosine analog, which prompted us to examine effects on FDG accumulation in cells pretreated with hexoses [15]. Similar induction of sugar transport was reported by Miller, who observed accelerated sugar out-flow [21].…”

Section: Discussionsupporting

confidence: 59%

“…14 C-FDG accumulation experiments were performed according to previously reported methods, with modifications [14,15]. For timecourse experiments, AsPC-1 cells were rinsed with D-PBS and then incubated with 2 ml D-PBS containing 14 C-FDG ( 14 C-FDG D-PBS; 9.25 kBq/ dish) at 37°C for a specific period (15 to 180 min).…”

Section: Characteristics Of 14 C-fdg Transport Into Aspc-1 Cellsmentioning

confidence: 99%

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A strategy for improving FDG accumulation for early detection of metastasis from primary pancreatic cancer: Stimulation of the Warburg effect in AsPC-1 cells

Ogura

Shikano

Nakajima

et al. 2015

Nuclear Medicine and Biology

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a b s t r a c t a r t i c l e i n f oIntroduction: Early detection and/or prediction of metastasis provide more prognostic relevance than local recurrence. Direct spread into the peritoneum is frequently found in pancreatic cancer patients, but positron emission tomography (PET) with 2-deoxy-2-fluoro-D-glucose (FDG) is not useful for identifying such metastasis. We investigated a method to enhance FDG accumulation using AsPC-1 human ascites tumor cells. Methods: 14 C-FDG accumulation was assessed under the following conditions: 1) characteristics of 14 C-FDG transport were examined using phloridzin, a Na + -free buffer, and various hexoses, and 2) accumulation of 14 C-FDG was measured in cells that were pretreated with hexose for various time periods, and activity of 6-phosphofructo-1-kinase (PFK-1) was assayed.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 59%

Section: Characteristics Of 14 C-fdg Transport Into Aspc-1 Cellsmentioning

confidence: 99%

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A strategy for improving FDG accumulation for early detection of metastasis from primary pancreatic cancer: Stimulation of the Warburg effect in AsPC-1 cells

Ogura

Shikano

Nakajima

et al. 2015

Nuclear Medicine and Biology

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“…The relative contributions of amino acid transport systems A, L, and, ASC/other to the uptake of [23] and Nakajima et al [24] and the results are shown in Table 1. Although the dominant uptake mechanism for [ 3 H]MET in human carcinomas was through system L amino acid transport, system A, ASC, or other Na + -dependent system(s) also played a role.…”

Section: Uptake Of [mentioning

confidence: 99%

“…BCH was used as a system L inhibitor and MeAIB was used as a system A inhibitor. To calculate the relative contributions of amino acid transporter systems, we used the methods reported by Shikano et al [23] and Nakajima et al [24]. In brief, uptake of mRNA levels of neutral amino acid transporters were determined by conducting qRT-PCR assays.…”

Section: C]met Has Been As Widely Used In Brain Tumor Imaging As O-(2mentioning

confidence: 99%

Relationship between [ 14 C]MeAIB uptake and amino acid transporter family gene expression levels or proliferative activity in a pilot study in human carcinoma cells: Comparison with [ 3 H]methionine uptake

Kagawa

Nishii

Higashi³

et al. 2017

Nuclear Medicine and Biology

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Results and Conclusion: [ 14 C]MeAIB uptake occurred principally via a Na + -dependent A type mechanism whereas [ 3 H]MET uptake, which occurred predominantly via a Na + -independent L type mechanism although other transporters were also utilized depending on cell type. There was no correlation between [ 3 H]MET uptake and total system L amino acid transporter (LAT) expression. In contrast, [ 14 C]MeAIB uptake strongly correlated with total system A amino acid transporter (SNAT) expression and proliferative activity in this preliminary study using four human carcinoma cell lines. Carcinoma proliferative activity also correlated with total SNAT expression.

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Intracellular boron accumulation in CHO-K1 cells using amino acid transport control

Sato

Yamamoto

Shikano

et al. 2014

Applied Radiation and Isotopes

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BPA used in BNCT has a similar structure to some essential amino acids and is transported into tumor cells by amino acid transport systems. Previous study groups have tried various techniques of loading BPA to increase intracellular boron concentration. CHO-K1 cells demonstrate system L (LAT1) activity and are suitable for specifying the transport system of a neutral amino acid. In this study, we examined the intracellular accumulation of boron in CHO-K1 cells by amino acid transport control, which involves co-loading with L-type amino acid esters. Intracellular boron accumulation in CHO-K1 cells showed the greatest increased upon co-loading 1.0mM BPA, with 1.0mM l-Tyr-O-Et and incubating for 60min. This increase is caused by activation of a system L amino acid exchanger between BPA and l-Tyr. The amino acid esters are metabolized to amino acids by intracellular hydrolytic enzymes that increase the concentrations of intracellular amino acids and stimulate exchange transportation. We expect that this amino acid transport control will be useful for enhancing intracellular boron accumulation.

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Stimulation of 125I-3-iodo-α-methyl-l-tyrosine uptake in Chinese hamster ovary (CHO-K1) cells by tyrosine esters

Cited by 7 publications

References 24 publications

A strategy for improving FDG accumulation for early detection of metastasis from primary pancreatic cancer: Stimulation of the Warburg effect in AsPC-1 cells

A strategy for improving FDG accumulation for early detection of metastasis from primary pancreatic cancer: Stimulation of the Warburg effect in AsPC-1 cells

Relationship between [ 14 C]MeAIB uptake and amino acid transporter family gene expression levels or proliferative activity in a pilot study in human carcinoma cells: Comparison with [ 3 H]methionine uptake

Intracellular boron accumulation in CHO-K1 cells using amino acid transport control

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