Stimulation by Proopiomelanocortin-Derived Peptides of LH Release by Bullfrog Dispersed Anterior Pituitary Cells

Aida, T; Oguchi, Atsuko; Iwamuro, Shawichi; Yamamoto, Kazuki; Kikuyama, Sakaé

doi:10.2108/zsj.14.827

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…Indeed, CRH is a well‐known stimulator of the secretion of ACTH, α ‐MSH and β ‐endorphin in vivo and in vitro in mammals ( 47) and in non‐mammalian species (teleosts (39, 40, 48); amphibians ( 49, 50); birds ( 51)). Furthermore, a GH‐releasing effect of α ‐MSH, involving a direct pituitary site of action, has been reported in human and rat ( 52); in the bullfrog ( Rana catesbeiana ), ACTH (1–17), but not α ‐MSH, stimulates GH release from dispersed pituitary cells ( 53). On the basis of these data, we investigated the possible action of POMC‐derived peptides, ACTH (1–17), α ‐MSH and β ‐endorphin on GH release.…”

Section: Discussionmentioning

confidence: 99%

Evidence That Corticotropin‐Releasing Hormone Acts as a Growth Hormone‐Releasing Factor in a Primitive Teleost, the European Eel (Anguilla anguilla)

Rousseau

Belle

Marchelidon

et al. 1999

J Neuroendocrinology

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The inhibitory control of growth hormone (GH) release by somatostatin (SRIH) has been conserved throughout vertebrate evolution. In contrast, the neuropeptides involved in the stimulatory control of GH vary according to species and/or physiological situations. We investigated the direct pituitary regulation of GH release in a primitive teleost, the European eel (Anguilla anguilla L.) at the juvenile stage. Short-term serum-free primary cultures of dispersed pituitary cells were used, and GH release was measured by an homologous radioimmunoassay. Whereas growth hormone-releasing hormone (GHRH), gonadotropin-releasing hormone (GnRH), thyrotropin-releasing hormone (TRH), neuropeptide Y (NPY) and cholecystokinin (CCK) failed to induce any change in GH release, corticotropin-releasing hormone (CRH) dose-dependently stimulated GH release with a significant effect at 1 nM and a maximal effect (> or =400% of controls at 24 h) at 100 nM. In agreement with our previous studies, PACAP also stimulated GH release but its maximal effect was lower than that of CRH. Proopiomelanocortin (POMC)-peptides, corticotropin (ACTH), melanotropin (alpha-MSH), beta-endorphin) had no effect on GH release, at any dose tested (0.1-1000 nM), indicating that the stimulatory effect of CRH on GH release by somatotrophs was not mediated by CRH-induced release of POMC-peptides from corticotrophs and melanotrophs. The CRH antagonist, alpha-helical CRH(9-41), significantly inhibited the stimulatory effect of CRH on GH release, suggesting the implication of specific CRH receptors related to mammalian ones. The stimulatory effect of CRH on GH release was reduced after 24 h of incubation, indicating a desensitization. In contrast, no desensitization to the inhibitory effect of SRIH was observed. SRIH inhibited CRH action in a dose-dependent manner. The effect of SRIH was overriding, 1 nM SRIH being able to abolish the effect of 1000 nM CRH. In conclusion, in the eel, CRH stimulates GH release directly at the pituitary cell level. GH and cortisol secretions could interact in controlling several physiological functions such as metabolism and ion exchange. This study suggests that CRH may have played an important early role in vertebrates co-ordinating the activation of various endocrine axes involved in metamorphosis, osmoregulation, stress and fasting. The stimulatory role of CRH on GH release may have been partially conserved during evolution, as it is found in some human physio-pathological situations such as stress, fasting and depression.

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Section: Discussionmentioning

confidence: 99%

Evidence That Corticotropin‐Releasing Hormone Acts as a Growth Hormone‐Releasing Factor in a Primitive Teleost, the European Eel (Anguilla anguilla)

Rousseau

Belle

Marchelidon

et al. 1999

J Neuroendocrinology

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“…When necessary, hGnRH or TRH was also used in order to secure the responsiveness of pituitary cells. Incubation was continued for 24 hr, since dispersed pituitary cells of the bullfrog are known to release various pituitary hormones continuously during this period Aida et al, 1997). After incubation, the medium was collected from each well and centrifuged, and the supernatant was subjected to radioimmunoassay (RIA) for bullfrog FSH (Polzonetti-Magni et al, 1998), LH , GH (Kobayashi et al, 1991) and PRL (Yamamoto and Kikuyama, 1982).…”

Section: Cell Incubation and Hormone Assaymentioning

confidence: 99%

Effect of Activin A and Follistatin on the Release of Pituitary Hormones in the Bullfrog Rana catesbeiana

et al. 2000

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Enhancement by Proopiomelanocortin-Derived Peptides of Growth Hormone and Prolactin Secretion by Bullfrog Pituitary Cells

Aida

Yamamoto

Kikuyama

1999

General and Comparative Endocrinology

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Stimulation by Proopiomelanocortin-Derived Peptides of LH Release by Bullfrog Dispersed Anterior Pituitary Cells

Cited by 6 publications

References 18 publications

Evidence That Corticotropin‐Releasing Hormone Acts as a Growth Hormone‐Releasing Factor in a Primitive Teleost, the European Eel (Anguilla anguilla)

Evidence That Corticotropin‐Releasing Hormone Acts as a Growth Hormone‐Releasing Factor in a Primitive Teleost, the European Eel (Anguilla anguilla)

Effect of Activin A and Follistatin on the Release of Pituitary Hormones in the Bullfrog Rana catesbeiana

Enhancement by Proopiomelanocortin-Derived Peptides of Growth Hormone and Prolactin Secretion by Bullfrog Pituitary Cells

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