Stimulation by Gangliosides of Viability of Rat Brain Neurons and of Neuronal PC12 Cell Line under Conditions of Oxidative Stress

Sokolova, T. V.; Furaev, V. V.; Victorov, Ilya V.; Andreeva, N. A.; Аврова, Н. Ф.

doi:10.1007/s10893-005-0077-4

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Gangliosides have been found to directly scavenge superoxide anions in the rat heart and to modulate signal transduction in rat brain synaptosomes. 33,37,63 Our study on human spermatozoa revealed that the exogenously added diand trisialogangliosides GD1b and GT1b could reduce the level of superoxide anions generated by phorbol,12-myristate acetate (PMA)-stimulated human spermatozoa. 17 Our recent research on ROS production in PMA-activated human leukocytes modulated by gangliosides demonstrated that exogenous GM1 and GT1b micelles could decrease the extracellular production of superoxide radicals in a Figure 2 The EPR study of DMPO-OH spin adduct formation in a Fenton-type reaction in the presence and absence of different concentrations of GM1 (&) and GT1b (%).…”

Section: Gangliosides and Spermatozoamentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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This article summarizes the available evidence on the efficacy of gangliosides to reduce the degree of reactive oxygen species (ROS)-mediated damage. The antioxidative efficacy of exogenous gangliosides in protecting different cells encouraged us to examine their ability to protect human spermatozoa. Gangliosides are sialic acid-containing glycosphingolipids with strong amphiphilic character due to the bulky headgroup made of several sugar rings with sialic acid residues and the double-tailed hydrophobic lipid moiety. The amphiphilicity of gangliosides allows them to exist as micelles in aqueous media when they are present at a concentration above their critical micellar concentration. The protective effect of ganglioside micelles on spermatozoa is believed to stem from their ability to scavenge free radicals and prevent their damaging effects. In our study, we particularly focused our attention on the protective effect of ganglioside micelles on DNA in human spermatozoa exposed to cryopreservation. The results indicate that ganglioside micelles can modulate the hydrophobic properties of the sperm membrane to increase tolerance to DNA fragmentation, thus protecting the DNA from cryopreservation-induced damage. Further actions of ganglioside micelles, which were documented by biochemical and biophysical studies, included (i) the modulation of superoxide anion generation by increasing the diffusion barrier for membrane events responsible for signal translocation to the interior of the cell; (ii) the inhibition of iron-catalysed hydroxyl radical formation due to the iron chelation potential of gangliosides; and (iii) inhibition of hydrogen peroxide diffusion across the sperm membrane.

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Section: Gangliosides and Spermatozoamentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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“…There is evidence of the ability of gangliosides at micromolar concentrations to protect neuronal cells from the effects of oxidative stress (16). Recently, we have found that ganglioside micelles act as inhibitors of reactive oxygen species (ROS) generation in a cell-free system (17).…”

Section: Introductionmentioning

confidence: 99%

Modulation of ROS production in human leukocytes by ganglioside micelles

Gavella

Kveder

Lipovac

2010

Braz J Med Biol Res

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Antioxidant properties of ganglioside micelles

Gavella

Kveder²,

Lipovac

et al. 2007

Free Radical Research

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Antioxidant activity of gangliosides GM1 and GT1b in the Fenton type of reaction was investigated by EPR spectroscopy using DMPO as a spin trap. Hydroxyl radical spin adduct signal intensity was significantly reduced in the presence of gangliosides at their micellar concentrations. Mean micellar hydrodynamic diameter was not changed, whereas significant changes in negative Zeta potential values were observed as evidenced by Zetasizer Nano ZS. This study showed that the primary mode of ganglioside action was not due to direct scavenging of OH., but rather to the inhibition of hydroxyl radical formation. This phenomenon is related to the ability of ganglioside micelles to bind oppositely charged ferrous ions, thus reducing their concentration and consequently inhibiting OH. formation.

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Stimulation by Gangliosides of Viability of Rat Brain Neurons and of Neuronal PC12 Cell Line under Conditions of Oxidative Stress

Cited by 3 publications

References 24 publications

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Modulation of ROS production in human leukocytes by ganglioside micelles

Antioxidant properties of ganglioside micelles

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